Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs
<i>Allium cepa</i> L. is one of the most abundant vegetable crops worldwide. In addition to its versatile culinary uses, onion also exhibits quite interesting medicinal uses. Bulbs have a high content of bioactive compounds that are beneficial for human health. This study intends to deve...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/10/11/1755 |
| _version_ | 1797511430527778816 |
|---|---|
| author | Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Estrella Espada-Bellido Marta Ferreiro-González Ceferino Carrera Gerardo F. Barbero Miguel Palma |
| author_facet | Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Estrella Espada-Bellido Marta Ferreiro-González Ceferino Carrera Gerardo F. Barbero Miguel Palma |
| author_sort | Ana V. González-de-Peredo |
| collection | DOAJ |
| description | <i>Allium cepa</i> L. is one of the most abundant vegetable crops worldwide. In addition to its versatile culinary uses, onion also exhibits quite interesting medicinal uses. Bulbs have a high content of bioactive compounds that are beneficial for human health. This study intends to develop and optimize two appropriate ultrasound-assisted methods for the extraction of the phenolic compounds and anthocyanins present in red onion. A response surface methodology was employed and, specifically, a Box–Behnken design, for the optimization of the methods. The optimal conditions for the extraction of the phenolic compounds were the follows: 53% MeOH as solvent, pH 2.6, 60 °C temperature, 30.1% amplitude, 0.43 s cycle, and 0.2:11 g sample/mL solvent ratio. On the other hand, the optimal conditions for the anthocyanins were as follows: 57% MeOH as solvent, pH 2, 60 °C temperature, 90% amplitude, 0.64 s cycle, and 0.2:15 g sample/mL solvent ratio. Both methods presented high repeatability and intermediate precision, as well as short extraction times with good recovery yields. These results illustrate that the use of ultrasound-assisted extraction, when properly optimized, is suitable for the extraction and quantification of the compounds of interest to determine and improve the quality of the raw material and its subproducts for consumers. |
| first_indexed | 2024-03-10T05:45:09Z |
| format | Article |
| id | doaj.art-66a933045a32411389dc49f19e285014 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T05:45:09Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-66a933045a32411389dc49f19e2850142023-11-22T22:13:13ZengMDPI AGAntioxidants2076-39212021-11-011011175510.3390/antiox10111755Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion BulbsAna V. González-de-Peredo0Mercedes Vázquez-Espinosa1Estrella Espada-Bellido2Marta Ferreiro-González3Ceferino Carrera4Gerardo F. Barbero5Miguel Palma6Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 Cadiz, Spain<i>Allium cepa</i> L. is one of the most abundant vegetable crops worldwide. In addition to its versatile culinary uses, onion also exhibits quite interesting medicinal uses. Bulbs have a high content of bioactive compounds that are beneficial for human health. This study intends to develop and optimize two appropriate ultrasound-assisted methods for the extraction of the phenolic compounds and anthocyanins present in red onion. A response surface methodology was employed and, specifically, a Box–Behnken design, for the optimization of the methods. The optimal conditions for the extraction of the phenolic compounds were the follows: 53% MeOH as solvent, pH 2.6, 60 °C temperature, 30.1% amplitude, 0.43 s cycle, and 0.2:11 g sample/mL solvent ratio. On the other hand, the optimal conditions for the anthocyanins were as follows: 57% MeOH as solvent, pH 2, 60 °C temperature, 90% amplitude, 0.64 s cycle, and 0.2:15 g sample/mL solvent ratio. Both methods presented high repeatability and intermediate precision, as well as short extraction times with good recovery yields. These results illustrate that the use of ultrasound-assisted extraction, when properly optimized, is suitable for the extraction and quantification of the compounds of interest to determine and improve the quality of the raw material and its subproducts for consumers.https://www.mdpi.com/2076-3921/10/11/1755<i>Allium cepa</i> L.anthocyaninsBox–Behnkenonionphenolic compoundsUHPLC |
| spellingShingle | Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Estrella Espada-Bellido Marta Ferreiro-González Ceferino Carrera Gerardo F. Barbero Miguel Palma Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs Antioxidants <i>Allium cepa</i> L. anthocyanins Box–Behnken onion phenolic compounds UHPLC |
| title | Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs |
| title_full | Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs |
| title_fullStr | Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs |
| title_full_unstemmed | Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs |
| title_short | Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs |
| title_sort | development of optimized ultrasound assisted extraction methods for the recovery of total phenolic compounds and anthocyanins from onion bulbs |
| topic | <i>Allium cepa</i> L. anthocyanins Box–Behnken onion phenolic compounds UHPLC |
| url | https://www.mdpi.com/2076-3921/10/11/1755 |
| work_keys_str_mv | AT anavgonzalezdeperedo developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT mercedesvazquezespinosa developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT estrellaespadabellido developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT martaferreirogonzalez developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT ceferinocarrera developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT gerardofbarbero developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs AT miguelpalma developmentofoptimizedultrasoundassistedextractionmethodsfortherecoveryoftotalphenoliccompoundsandanthocyaninsfromonionbulbs |