Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE
Objective To evaluate the efficacy of combined rapid on-site evaluation of cytology (ROSE), ultrathin bronchoscopy, virtual bronchoscopic navigation, radial endobronchial ultrasound (EBUS), and metagenomic next-generation sequencing (mNGS) for diagnosis of peripheral pulmonary infectious lesions. Me...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2019-10-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060519866953 |
| _version_ | 1819143229954588672 |
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| author | Nana Liu Jianying Kan Wenbin Cao Jie Cao Erlie Jiang Yang Zhou Mingfeng Zhao Jing Feng |
| author_facet | Nana Liu Jianying Kan Wenbin Cao Jie Cao Erlie Jiang Yang Zhou Mingfeng Zhao Jing Feng |
| author_sort | Nana Liu |
| collection | DOAJ |
| description | Objective To evaluate the efficacy of combined rapid on-site evaluation of cytology (ROSE), ultrathin bronchoscopy, virtual bronchoscopic navigation, radial endobronchial ultrasound (EBUS), and metagenomic next-generation sequencing (mNGS) for diagnosis of peripheral pulmonary infectious lesions. Methods Specimens from patients with peripheral lung infection were obtained by transbronchial lung biopsy (TBLB) and bronchoalveolar lavage (BAL), and mNGS was used to detect pathogenic microorganisms. The sensitivity and specificity of mNGS were compared between TBLB tissue and BAL fluid. Results The most common pathogens of pulmonary infectious lesions in this study were Pseudomonas aeruginosa , Klebsiella pneumoniae , and Acinetobacter baumannii . The specificity of mNGS was higher in TBLB tissue than in BAL fluid, but mNGS of BAL fluid had higher sensitivity. Conclusions The combination of ROSE, ultrathin bronchoscopy, virtual bronchoscopic navigation, radial EBUS, and mNGS technology yielded high efficacy for the diagnosis of peripheral pulmonary infectious lesions. TBLB and BAL specimens have respective advantages in specificity and sensitivity for mNGS analysis. |
| first_indexed | 2024-12-22T12:22:56Z |
| format | Article |
| id | doaj.art-66aa4f9b006d4a7d8e7e86813f5f6bca |
| institution | Directory Open Access Journal |
| issn | 0300-0605 1473-2300 |
| language | English |
| last_indexed | 2024-12-22T12:22:56Z |
| publishDate | 2019-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-66aa4f9b006d4a7d8e7e86813f5f6bca2022-12-21T18:25:55ZengSAGE PublishingJournal of International Medical Research0300-06051473-23002019-10-014710.1177/0300060519866953Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSENana LiuJianying KanWenbin CaoJie CaoErlie JiangYang ZhouMingfeng ZhaoJing FengObjective To evaluate the efficacy of combined rapid on-site evaluation of cytology (ROSE), ultrathin bronchoscopy, virtual bronchoscopic navigation, radial endobronchial ultrasound (EBUS), and metagenomic next-generation sequencing (mNGS) for diagnosis of peripheral pulmonary infectious lesions. Methods Specimens from patients with peripheral lung infection were obtained by transbronchial lung biopsy (TBLB) and bronchoalveolar lavage (BAL), and mNGS was used to detect pathogenic microorganisms. The sensitivity and specificity of mNGS were compared between TBLB tissue and BAL fluid. Results The most common pathogens of pulmonary infectious lesions in this study were Pseudomonas aeruginosa , Klebsiella pneumoniae , and Acinetobacter baumannii . The specificity of mNGS was higher in TBLB tissue than in BAL fluid, but mNGS of BAL fluid had higher sensitivity. Conclusions The combination of ROSE, ultrathin bronchoscopy, virtual bronchoscopic navigation, radial EBUS, and mNGS technology yielded high efficacy for the diagnosis of peripheral pulmonary infectious lesions. TBLB and BAL specimens have respective advantages in specificity and sensitivity for mNGS analysis.https://doi.org/10.1177/0300060519866953 |
| spellingShingle | Nana Liu Jianying Kan Wenbin Cao Jie Cao Erlie Jiang Yang Zhou Mingfeng Zhao Jing Feng Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE Journal of International Medical Research |
| title | Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE |
| title_full | Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE |
| title_fullStr | Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE |
| title_full_unstemmed | Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE |
| title_short | Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE |
| title_sort | metagenomic next generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation radial ebus ultrathin bronchoscopy and rose |
| url | https://doi.org/10.1177/0300060519866953 |
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