Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times
Background: Tobacco is a plant that has anti-nematode and anti-inflammatory properties, due to that it contains compounds such as nicotine sulfate. It is utilized as a medicinal plant in the therapy of infectious diseases; also, it has been used to kill insects’ larvae and plant pests. Objective: Th...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2022-05-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-3309-en.html |
| _version_ | 1818002517001240576 |
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| author | Roya Latifi Abbas Azadmehr Sahar Mosalla Kiarash Saleki Reza Hajiaghaee |
| author_facet | Roya Latifi Abbas Azadmehr Sahar Mosalla Kiarash Saleki Reza Hajiaghaee |
| author_sort | Roya Latifi |
| collection | DOAJ |
| description | Background: Tobacco is a plant that has anti-nematode and anti-inflammatory properties, due to that it contains compounds such as nicotine sulfate. It is utilized as a medicinal plant in the therapy of infectious diseases; also, it has been used to kill insects’ larvae and plant pests. Objective: The goal of this work was the evaluation of tobacco extract effects on the scolices of hydatid cysts. Methods: Hydatid cysts of the infected sheep livers were gathered from the Qazvin abattoir and their protoscolices were isolated under sterile conditions. Fertility and viability of protoscolices were confirmed before the experiments. The protoscolices were exposed to the ethanolic extract of Nicotiana tabacum, at different concentrations (3, 5, 10, 25, 50, and 100 mg/ml) and various exposure times (10, 20, 30, 40, 50 and 60 min). Sodium chloride 0.9 % was selected as negative control. The viability of protoscolices was evaluated by staining via 0.1 % eosin. Results: At 100 mg/ml, the tobacco extract caused the death of 91.19 % of protoscolices after 10 minutes of contact, and other exposure times (20 to 60 minutes) resulted in the death of 100 % of protoscolices. Conclusion: This medicinal plant showed a good scolicidal effect and it may be used for improving the procedure of hydatid cyst surgery. But, further studies are required. |
| first_indexed | 2024-04-14T03:46:49Z |
| format | Article |
| id | doaj.art-66aa6250c2854366b4cb57c2dd653b32 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-04-14T03:46:49Z |
| publishDate | 2022-05-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-66aa6250c2854366b4cb57c2dd653b322022-12-22T02:14:13ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582022-05-012182111118Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure timesRoya Latifi0Abbas Azadmehr1Sahar Mosalla2Kiarash Saleki3Reza Hajiaghaee4 Parasitology Department, Qazvin University of Medical Sciences, Qazvin, Iran Immunology Department, Babol University of Medical Sciences, Babol, Iran & Immunology Department, Qazvin University of Medical Sciences, Qazvin, Iran Parasitology Department, Qazvin University of Medical Sciences, Qazvin, Iran Student Research Committee, Babol University of Medical Sciences, Babol, Iran & USERN Office, Babol University of Medical Sciences, Babol, Iran Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Background: Tobacco is a plant that has anti-nematode and anti-inflammatory properties, due to that it contains compounds such as nicotine sulfate. It is utilized as a medicinal plant in the therapy of infectious diseases; also, it has been used to kill insects’ larvae and plant pests. Objective: The goal of this work was the evaluation of tobacco extract effects on the scolices of hydatid cysts. Methods: Hydatid cysts of the infected sheep livers were gathered from the Qazvin abattoir and their protoscolices were isolated under sterile conditions. Fertility and viability of protoscolices were confirmed before the experiments. The protoscolices were exposed to the ethanolic extract of Nicotiana tabacum, at different concentrations (3, 5, 10, 25, 50, and 100 mg/ml) and various exposure times (10, 20, 30, 40, 50 and 60 min). Sodium chloride 0.9 % was selected as negative control. The viability of protoscolices was evaluated by staining via 0.1 % eosin. Results: At 100 mg/ml, the tobacco extract caused the death of 91.19 % of protoscolices after 10 minutes of contact, and other exposure times (20 to 60 minutes) resulted in the death of 100 % of protoscolices. Conclusion: This medicinal plant showed a good scolicidal effect and it may be used for improving the procedure of hydatid cyst surgery. But, further studies are required.http://jmp.ir/article-1-3309-en.htmlherbal medicinetobaccohydatic cystscoliocidal treatmentimmunotoxicity |
| spellingShingle | Roya Latifi Abbas Azadmehr Sahar Mosalla Kiarash Saleki Reza Hajiaghaee Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times Journal of Medicinal Plants herbal medicine tobacco hydatic cyst scoliocidal treatment immunotoxicity |
| title | Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times |
| title_full | Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times |
| title_fullStr | Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times |
| title_full_unstemmed | Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times |
| title_short | Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times |
| title_sort | scolicidal effects of the nicotiana tabacum l extract at various concentrations and exposure times |
| topic | herbal medicine tobacco hydatic cyst scoliocidal treatment immunotoxicity |
| url | http://jmp.ir/article-1-3309-en.html |
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