May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology
For more than a couple of decades now, “force” has been recognized as an important physical parameter that cells employ to adapt to their microenvironment. Whether it is externally applied, or internally generated, cells use force to modulate their various actions, from adhesion and migration to dif...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.898558/full |
| _version_ | 1828150026371596288 |
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| author | Farah Mustapha Farah Mustapha Farah Mustapha Kheya Sengupta Kheya Sengupta Pierre-Henri Puech Pierre-Henri Puech |
| author_facet | Farah Mustapha Farah Mustapha Farah Mustapha Kheya Sengupta Kheya Sengupta Pierre-Henri Puech Pierre-Henri Puech |
| author_sort | Farah Mustapha |
| collection | DOAJ |
| description | For more than a couple of decades now, “force” has been recognized as an important physical parameter that cells employ to adapt to their microenvironment. Whether it is externally applied, or internally generated, cells use force to modulate their various actions, from adhesion and migration to differentiation and immune function. T lymphocytes use such mechano-sensitivity to decipher signals when recognizing cognate antigens presented on the surface of antigen presenting cells (APCs), a critical process in the adaptive immune response. As such, many techniques have been developed and used to measure the forces felt/exerted by these small, solitary and extremely reactive cells to decipher their influence on diverse T cell functions, primarily activation. Here, we focus on traction force microscopy (TFM), in which a deformable substrate, coated with the appropriate molecules, acts as a force sensor on the cellular scale. This technique has recently become a center of interest for many groups in the “ImmunoBiophysics” community and, as a consequence, has been subjected to refinements for its application to immune cells. Here, we present an overview of TFM, the precautions and pitfalls, and the most recent developments in the context of T cell immunology. |
| first_indexed | 2024-04-11T21:38:34Z |
| format | Article |
| id | doaj.art-66aac8fe81f143baaf70847f33be9085 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T21:38:34Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-66aac8fe81f143baaf70847f33be90852022-12-22T04:01:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.898558898558May the force be with your (immune) cells: an introduction to traction force microscopy in ImmunologyFarah Mustapha0Farah Mustapha1Farah Mustapha2Kheya Sengupta3Kheya Sengupta4Pierre-Henri Puech5Pierre-Henri Puech6Laboratory Adhesion Inflammation (LAI), INSERM, CNRS, Aix Marseille University, Marseille, FranceCentre Interdisciplinaire de Nanoscience de Marseille (CINaM), CNRS, Aix Marseille University, Marseille, FranceTuring Center for Living Systems (CENTURI), Marseille, FranceCentre Interdisciplinaire de Nanoscience de Marseille (CINaM), CNRS, Aix Marseille University, Marseille, FranceTuring Center for Living Systems (CENTURI), Marseille, FranceLaboratory Adhesion Inflammation (LAI), INSERM, CNRS, Aix Marseille University, Marseille, FranceTuring Center for Living Systems (CENTURI), Marseille, FranceFor more than a couple of decades now, “force” has been recognized as an important physical parameter that cells employ to adapt to their microenvironment. Whether it is externally applied, or internally generated, cells use force to modulate their various actions, from adhesion and migration to differentiation and immune function. T lymphocytes use such mechano-sensitivity to decipher signals when recognizing cognate antigens presented on the surface of antigen presenting cells (APCs), a critical process in the adaptive immune response. As such, many techniques have been developed and used to measure the forces felt/exerted by these small, solitary and extremely reactive cells to decipher their influence on diverse T cell functions, primarily activation. Here, we focus on traction force microscopy (TFM), in which a deformable substrate, coated with the appropriate molecules, acts as a force sensor on the cellular scale. This technique has recently become a center of interest for many groups in the “ImmunoBiophysics” community and, as a consequence, has been subjected to refinements for its application to immune cells. Here, we present an overview of TFM, the precautions and pitfalls, and the most recent developments in the context of T cell immunology.https://www.frontiersin.org/articles/10.3389/fimmu.2022.898558/fulltraction force microscopy (TFM)immune cellmechanicsforcemechanobiology |
| spellingShingle | Farah Mustapha Farah Mustapha Farah Mustapha Kheya Sengupta Kheya Sengupta Pierre-Henri Puech Pierre-Henri Puech May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology Frontiers in Immunology traction force microscopy (TFM) immune cell mechanics force mechanobiology |
| title | May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology |
| title_full | May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology |
| title_fullStr | May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology |
| title_full_unstemmed | May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology |
| title_short | May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology |
| title_sort | may the force be with your immune cells an introduction to traction force microscopy in immunology |
| topic | traction force microscopy (TFM) immune cell mechanics force mechanobiology |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.898558/full |
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