Can telomere shortening be the main indicator of non-viable fetus elimination?
Abstract Background Telomeres are transcriptionally inactive genomic areas, which, if shortened, are associated with pathological processes, unsuccessful fertilization, aging, and death. Telomere dysfunction has also been linked to chromosomal rearrangements and genomic instability. The role of telo...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Molecular Cytogenetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13039-018-0361-9 |
| _version_ | 1811215918763606016 |
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| author | Nataliya Huleyuk Iryna Tkach Danuta Zastavna Miroslaw Tyrka |
| author_facet | Nataliya Huleyuk Iryna Tkach Danuta Zastavna Miroslaw Tyrka |
| author_sort | Nataliya Huleyuk |
| collection | DOAJ |
| description | Abstract Background Telomeres are transcriptionally inactive genomic areas, which, if shortened, are associated with pathological processes, unsuccessful fertilization, aging, and death. Telomere dysfunction has also been linked to chromosomal rearrangements and genomic instability. The role of telomeres in postnatal life has been extensively studied and discussed both in physiological as well as in pathological processes. However, the role of telomere length in prenatal development is still poorly understood, and mainly concerns the preimplantation stage. The aim of this study was to estimate relative telomere length in spontaneously eliminated human embryos between 5th and 12th week of gestation. Results Relative telomere length was measured from total genomic DNA using a real-time polymerase chain reaction approach. In this study, we examined relative telomere length in 80 spontaneously eliminated embryos and in 25 embryos eliminated due to induced abortions. Relative telomere length in spontaneous abortions was significantly lower (P = 0.000001) compared to the induced abortions. Spontaneous abortions with aneuploid anomalies (monosomy X, trisomy 21, trisomy 16 and triploidy) were characterized by shorter telomeres, compared to spontaneous abortions, subgroup with euploid (46,XN) karyotype. Conclusion Spontaneously lost pregnancies are characterized by shortened telomeres, especially in embryos with aneuploidies. We hypothesize that the shortening of telomeres is involved in the processes leading to spontaneous abortions. |
| first_indexed | 2024-04-12T06:31:24Z |
| format | Article |
| id | doaj.art-66ab34b171de45979bcd59ea1b60cc5f |
| institution | Directory Open Access Journal |
| issn | 1755-8166 |
| language | English |
| last_indexed | 2024-04-12T06:31:24Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cytogenetics |
| spelling | doaj.art-66ab34b171de45979bcd59ea1b60cc5f2022-12-22T03:44:00ZengBMCMolecular Cytogenetics1755-81662018-01-011111710.1186/s13039-018-0361-9Can telomere shortening be the main indicator of non-viable fetus elimination?Nataliya Huleyuk0Iryna Tkach1Danuta Zastavna2Miroslaw Tyrka3Institute of Hereditary Pathology, NAMS of UkraineInstitute of Hereditary Pathology, NAMS of UkraineInstitute of Hereditary Pathology, NAMS of UkraineDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of TechnologyAbstract Background Telomeres are transcriptionally inactive genomic areas, which, if shortened, are associated with pathological processes, unsuccessful fertilization, aging, and death. Telomere dysfunction has also been linked to chromosomal rearrangements and genomic instability. The role of telomeres in postnatal life has been extensively studied and discussed both in physiological as well as in pathological processes. However, the role of telomere length in prenatal development is still poorly understood, and mainly concerns the preimplantation stage. The aim of this study was to estimate relative telomere length in spontaneously eliminated human embryos between 5th and 12th week of gestation. Results Relative telomere length was measured from total genomic DNA using a real-time polymerase chain reaction approach. In this study, we examined relative telomere length in 80 spontaneously eliminated embryos and in 25 embryos eliminated due to induced abortions. Relative telomere length in spontaneous abortions was significantly lower (P = 0.000001) compared to the induced abortions. Spontaneous abortions with aneuploid anomalies (monosomy X, trisomy 21, trisomy 16 and triploidy) were characterized by shorter telomeres, compared to spontaneous abortions, subgroup with euploid (46,XN) karyotype. Conclusion Spontaneously lost pregnancies are characterized by shortened telomeres, especially in embryos with aneuploidies. We hypothesize that the shortening of telomeres is involved in the processes leading to spontaneous abortions.http://link.springer.com/article/10.1186/s13039-018-0361-9Relative telomere lengthSpontaneous abortionsAneuploidyEuploidy |
| spellingShingle | Nataliya Huleyuk Iryna Tkach Danuta Zastavna Miroslaw Tyrka Can telomere shortening be the main indicator of non-viable fetus elimination? Molecular Cytogenetics Relative telomere length Spontaneous abortions Aneuploidy Euploidy |
| title | Can telomere shortening be the main indicator of non-viable fetus elimination? |
| title_full | Can telomere shortening be the main indicator of non-viable fetus elimination? |
| title_fullStr | Can telomere shortening be the main indicator of non-viable fetus elimination? |
| title_full_unstemmed | Can telomere shortening be the main indicator of non-viable fetus elimination? |
| title_short | Can telomere shortening be the main indicator of non-viable fetus elimination? |
| title_sort | can telomere shortening be the main indicator of non viable fetus elimination |
| topic | Relative telomere length Spontaneous abortions Aneuploidy Euploidy |
| url | http://link.springer.com/article/10.1186/s13039-018-0361-9 |
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