Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm
This study aimed to determine the bactericidal efficacy of sequential use of NaOCl pH 12 followed by acidified NaOCl pH 6.5, and compare it to that of either of these NaOCl solutions alone. <i>E. faecalis</i> biofilm was grown on standardized dentine specimens for four weeks. The specime...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2076-3417/10/17/6096 |
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| author | Ronald Wigler Shlomo Matalon Tomer Goldberger Anat Or Lerner Anda Kfir |
| author_facet | Ronald Wigler Shlomo Matalon Tomer Goldberger Anat Or Lerner Anda Kfir |
| author_sort | Ronald Wigler |
| collection | DOAJ |
| description | This study aimed to determine the bactericidal efficacy of sequential use of NaOCl pH 12 followed by acidified NaOCl pH 6.5, and compare it to that of either of these NaOCl solutions alone. <i>E. faecalis</i> biofilm was grown on standardized dentine specimens for four weeks. The specimens were randomly divided into four groups: (A) 4 min exposure to 0.9% saline solution (control); (B) 4 min exposure to 4% NaOCl pH 12; (C) 4 min exposure to 4% NaOCl pH 6.5; and (D) 2 min exposure to 4% NaOCl pH 12 followed by 2 min exposure to 4% NaOCl pH 6.5. The bactericidal activity was evaluated after the 4 min of contact time using confocal laser scanning microscopy. The volume ratio of red fluorescence to green and red fluorescence indicated the proportion of dead cells in the biofilm. The percent of dead cells in the saline solution group was significantly lower than those in the other groups. There was no significant difference between NaOCl pH 12 compared to NaOCl pH 6.5. The sequential use of NaOCl pH 12 followed by pH 6.5 significantly increased the percent of dead cells compared to both the samples exposed to either NaOCl pH 12 or pH 6.5. These results show that sequential irrigation protocol had a stronger bactericidal effect than the commonly used NaOCl pH 12. |
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| language | English |
| last_indexed | 2024-03-10T16:37:50Z |
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| spelling | doaj.art-66ada593e81944a49cc3a456c9ee62e72023-11-20T12:19:25ZengMDPI AGApplied Sciences2076-34172020-09-011017609610.3390/app10176096Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> BiofilmRonald Wigler0Shlomo Matalon1Tomer Goldberger2Anat Or Lerner3Anda Kfir4Department of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6934228, IsraelDepartment of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6934228, IsraelDepartment of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6934228, IsraelDepartment of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6934228, IsraelDepartment of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6934228, IsraelThis study aimed to determine the bactericidal efficacy of sequential use of NaOCl pH 12 followed by acidified NaOCl pH 6.5, and compare it to that of either of these NaOCl solutions alone. <i>E. faecalis</i> biofilm was grown on standardized dentine specimens for four weeks. The specimens were randomly divided into four groups: (A) 4 min exposure to 0.9% saline solution (control); (B) 4 min exposure to 4% NaOCl pH 12; (C) 4 min exposure to 4% NaOCl pH 6.5; and (D) 2 min exposure to 4% NaOCl pH 12 followed by 2 min exposure to 4% NaOCl pH 6.5. The bactericidal activity was evaluated after the 4 min of contact time using confocal laser scanning microscopy. The volume ratio of red fluorescence to green and red fluorescence indicated the proportion of dead cells in the biofilm. The percent of dead cells in the saline solution group was significantly lower than those in the other groups. There was no significant difference between NaOCl pH 12 compared to NaOCl pH 6.5. The sequential use of NaOCl pH 12 followed by pH 6.5 significantly increased the percent of dead cells compared to both the samples exposed to either NaOCl pH 12 or pH 6.5. These results show that sequential irrigation protocol had a stronger bactericidal effect than the commonly used NaOCl pH 12.https://www.mdpi.com/2076-3417/10/17/6096bactericidal efficacybiofilmconfocal laser scanning microscopy<i>Enterococcus faecalis</i>NaOClpH |
| spellingShingle | Ronald Wigler Shlomo Matalon Tomer Goldberger Anat Or Lerner Anda Kfir Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm Applied Sciences bactericidal efficacy biofilm confocal laser scanning microscopy <i>Enterococcus faecalis</i> NaOCl pH |
| title | Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm |
| title_full | Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm |
| title_fullStr | Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm |
| title_full_unstemmed | Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm |
| title_short | Enhanced Bactericidal Efficacy of NaOCl at pH 12 Followed by Acidified NaOCl at pH 6.5 on <i>Enterococcus faecalis</i> Biofilm |
| title_sort | enhanced bactericidal efficacy of naocl at ph 12 followed by acidified naocl at ph 6 5 on i enterococcus faecalis i biofilm |
| topic | bactericidal efficacy biofilm confocal laser scanning microscopy <i>Enterococcus faecalis</i> NaOCl pH |
| url | https://www.mdpi.com/2076-3417/10/17/6096 |
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