Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China
Previous research on antibiotic resistance genes and microorganisms centered on those in urban sewage treatment plants, breeding farms, hospitals and others with serious antibiotic pollution. However, at present, there are evident proofs that antibiotic resistance genes (ARGs) indeed exist in a prim...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322010508 |
| _version_ | 1811312243667632128 |
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| author | Jia Ai Yancheng Li Yang Lv Xiong Zhong Jiang Li Aijiang Yang |
| author_facet | Jia Ai Yancheng Li Yang Lv Xiong Zhong Jiang Li Aijiang Yang |
| author_sort | Jia Ai |
| collection | DOAJ |
| description | Previous research on antibiotic resistance genes and microorganisms centered on those in urban sewage treatment plants, breeding farms, hospitals and others with serious antibiotic pollution. However, at present, there are evident proofs that antibiotic resistance genes (ARGs) indeed exist in a primitive environment hardly without any human’s footprints. Accordingly, an original karst mountain swamp ecosystem in Niangniang Mountain, Guizhou, China, including herbaceous swamp, shrub swamp, sphagnum bog and forest swamp, was selected to analyze the physical and chemical parameters of sediments. Moreover, microbial compositions, functions, as well as their connections with ARGs were assayed and analyzed using metagenomic technology. The results showed that there was no significant difference in the dominant microorganisms and ARGs in the four marshes, in which the dominant bacteria phyla were Proteobacteria (37.82 %), Acidobacteriota (22.17 %) and Actinobacteriota (20.64 %); the dominant archaea Euryarchaeota. (1.00 %); and the dominant eukaryotes Ascomycota (0.07 %), with metabolism as their major functions. Based on the ARDB database, the number of ARGs annotated reached 209 including 30 subtypes, and the dominant ARGs were all Bacitracin resistance genes (bacA, 84.77 %). In terms of the diversity of microorganisms and ARGs, the herbaceous swamp ranked the top, and the shrub swamp were at the bottom. Correlation analysis between microorganisms and resistance genes showed that, apart from aac2ic, macB, smeE, tetQ, and tetL, other ARGs were positively correlated with microorganisms. Among them, baca coexisted with microorganisms. Pearson correlation analysis results showed that contrary to ARGs, microorganisms were more affected by environmental factors. |
| first_indexed | 2024-04-13T10:32:43Z |
| format | Article |
| id | doaj.art-66b6ab0415a84f67babd9d2d8366468f |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-13T10:32:43Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-66b6ab0415a84f67babd9d2d8366468f2022-12-22T02:50:08ZengElsevierEcotoxicology and Environmental Safety0147-65132022-12-01247114210Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, ChinaJia Ai0Yancheng Li1Yang Lv2Xiong Zhong3Jiang Li4Aijiang Yang5College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China; Qiannan Ecological Environment Monitoring Center, Duyun 558000, ChinaCollege of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China; Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang 550025, China; Corresponding author at: College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China.College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, ChinaCollege of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, ChinaCollege of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China; Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang 550025, ChinaCollege of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China; Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang 550025, ChinaPrevious research on antibiotic resistance genes and microorganisms centered on those in urban sewage treatment plants, breeding farms, hospitals and others with serious antibiotic pollution. However, at present, there are evident proofs that antibiotic resistance genes (ARGs) indeed exist in a primitive environment hardly without any human’s footprints. Accordingly, an original karst mountain swamp ecosystem in Niangniang Mountain, Guizhou, China, including herbaceous swamp, shrub swamp, sphagnum bog and forest swamp, was selected to analyze the physical and chemical parameters of sediments. Moreover, microbial compositions, functions, as well as their connections with ARGs were assayed and analyzed using metagenomic technology. The results showed that there was no significant difference in the dominant microorganisms and ARGs in the four marshes, in which the dominant bacteria phyla were Proteobacteria (37.82 %), Acidobacteriota (22.17 %) and Actinobacteriota (20.64 %); the dominant archaea Euryarchaeota. (1.00 %); and the dominant eukaryotes Ascomycota (0.07 %), with metabolism as their major functions. Based on the ARDB database, the number of ARGs annotated reached 209 including 30 subtypes, and the dominant ARGs were all Bacitracin resistance genes (bacA, 84.77 %). In terms of the diversity of microorganisms and ARGs, the herbaceous swamp ranked the top, and the shrub swamp were at the bottom. Correlation analysis between microorganisms and resistance genes showed that, apart from aac2ic, macB, smeE, tetQ, and tetL, other ARGs were positively correlated with microorganisms. Among them, baca coexisted with microorganisms. Pearson correlation analysis results showed that contrary to ARGs, microorganisms were more affected by environmental factors.http://www.sciencedirect.com/science/article/pii/S0147651322010508KarstPrimitive plateau swampMicroorganismAntibiotic resistance geneNiangniang Mountain |
| spellingShingle | Jia Ai Yancheng Li Yang Lv Xiong Zhong Jiang Li Aijiang Yang Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China Ecotoxicology and Environmental Safety Karst Primitive plateau swamp Microorganism Antibiotic resistance gene Niangniang Mountain |
| title | Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China |
| title_full | Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China |
| title_fullStr | Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China |
| title_full_unstemmed | Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China |
| title_short | Study on microbes and antibiotic resistance genes in karst primitive mountain marshes – A case study of Niangniang Mountain in Guizhou, China |
| title_sort | study on microbes and antibiotic resistance genes in karst primitive mountain marshes a case study of niangniang mountain in guizhou china |
| topic | Karst Primitive plateau swamp Microorganism Antibiotic resistance gene Niangniang Mountain |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322010508 |
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