Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells
Adipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/10/611 |
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| author | Franziska B. Albrecht Freia F. Schmidt Ann-Cathrin Volz Petra J. Kluger |
| author_facet | Franziska B. Albrecht Freia F. Schmidt Ann-Cathrin Volz Petra J. Kluger |
| author_sort | Franziska B. Albrecht |
| collection | DOAJ |
| description | Adipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adipose tissue models. During culture, lobule exhibited a stable weight, lactate dehydrogenase, and glycerol release over 15 days. For building up in vitro adipose tissue models, we adapted the biomaterial gelatin methacryloyl (GelMA) composition and handling to homogeneously mix and bioprint human primary mature adipocytes (MA) and adipose-derived stem cells (ASCs), respectively. Accelerated cooling of the bioink turned out to be essential for the homogeneous distribution of lipid-filled MAs in the hydrogel. Last, we compared manual and bioprinted GelMA hydrogels with MA or ASCs and the explanted lobules to evaluate the impact of the printing process and rate the models concerning the physiological reference. The viability analyses demonstrated no significant difference between the groups due to additive manufacturing. The staining of intracellular lipids and perilipin A suggest that GelMA is well suited for ASCs and MA. Therefore, we successfully constructed physiological in vitro models by bioprinting MA-containing GelMA bioinks. |
| first_indexed | 2024-03-09T20:11:38Z |
| format | Article |
| id | doaj.art-66b72e4ed23a47c38e4c1173de01ebf5 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T20:11:38Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-66b72e4ed23a47c38e4c1173de01ebf52023-11-24T00:13:13ZengMDPI AGGels2310-28612022-09-0181061110.3390/gels8100611Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem CellsFranziska B. Albrecht0Freia F. Schmidt1Ann-Cathrin Volz2Petra J. Kluger3Reutlingen Research Institute, Reutlingen University, 72762 Reutlingen, GermanyReutlingen Research Institute, Reutlingen University, 72762 Reutlingen, GermanyReutlingen Research Institute, Reutlingen University, 72762 Reutlingen, GermanyFaculty of Applied Chemistry, Reutlingen University, 72762 Reutlingen, GermanyAdipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as an adipose tissue control to evaluate in vitro generated adipose tissue models. During culture, lobule exhibited a stable weight, lactate dehydrogenase, and glycerol release over 15 days. For building up in vitro adipose tissue models, we adapted the biomaterial gelatin methacryloyl (GelMA) composition and handling to homogeneously mix and bioprint human primary mature adipocytes (MA) and adipose-derived stem cells (ASCs), respectively. Accelerated cooling of the bioink turned out to be essential for the homogeneous distribution of lipid-filled MAs in the hydrogel. Last, we compared manual and bioprinted GelMA hydrogels with MA or ASCs and the explanted lobules to evaluate the impact of the printing process and rate the models concerning the physiological reference. The viability analyses demonstrated no significant difference between the groups due to additive manufacturing. The staining of intracellular lipids and perilipin A suggest that GelMA is well suited for ASCs and MA. Therefore, we successfully constructed physiological in vitro models by bioprinting MA-containing GelMA bioinks.https://www.mdpi.com/2310-2861/8/10/611adipose tissue3D bioprintinghydrogelsprimary human adipose-derived stem cellsprimary human mature adipocytesgelatin methacryloyl |
| spellingShingle | Franziska B. Albrecht Freia F. Schmidt Ann-Cathrin Volz Petra J. Kluger Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells Gels adipose tissue 3D bioprinting hydrogels primary human adipose-derived stem cells primary human mature adipocytes gelatin methacryloyl |
| title | Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells |
| title_full | Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells |
| title_fullStr | Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells |
| title_full_unstemmed | Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells |
| title_short | Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells |
| title_sort | bioprinting of 3d adipose tissue models using a gelma bioink with human mature adipocytes or human adipose derived stem cells |
| topic | adipose tissue 3D bioprinting hydrogels primary human adipose-derived stem cells primary human mature adipocytes gelatin methacryloyl |
| url | https://www.mdpi.com/2310-2861/8/10/611 |
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