The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells
Mesenchymal stromal cells (MSCs) have the potential to differentiate into a variety of mature cell types and are a promising source of regenerative medicine. The success of regenerative medicine using MSCs strongly depends on their differentiation potential. In this study, we sought to identify mark...
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MDPI AG
2021-11-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/43/3/150 |
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| author | Masami Kanawa Akira Igarashi Katsumi Fujimoto Tania Saskianti Ayumu Nakashima Yukihito Higashi Hidemi Kurihara Yukio Kato Takeshi Kawamoto |
| author_facet | Masami Kanawa Akira Igarashi Katsumi Fujimoto Tania Saskianti Ayumu Nakashima Yukihito Higashi Hidemi Kurihara Yukio Kato Takeshi Kawamoto |
| author_sort | Masami Kanawa |
| collection | DOAJ |
| description | Mesenchymal stromal cells (MSCs) have the potential to differentiate into a variety of mature cell types and are a promising source of regenerative medicine. The success of regenerative medicine using MSCs strongly depends on their differentiation potential. In this study, we sought to identify marker genes for predicting the osteogenic differentiation potential by comparing ilium MSC and fibroblast samples. We measured the mRNA levels of 95 candidate genes in nine ilium MSC and four fibroblast samples before osteogenic induction, and compared them with alkaline phosphatase (ALP) activity as a marker of osteogenic differentiation after induction. We identified 17 genes whose mRNA expression levels positively correlated with ALP activity. The chondrogenic and adipogenic differentiation potentials of jaw MSCs are much lower than those of ilium MSCs, although the osteogenic differentiation potential of jaw MSCs is comparable with that of ilium MSCs. To select markers suitable for predicting the osteogenic differentiation potential, we compared the mRNA levels of the 17 genes in ilium MSCs with those in jaw MSCs. The levels of 7 out of the 17 genes were not substantially different between the jaw and ilium MSCs, while the remaining 10 genes were expressed at significantly lower levels in jaw MSCs than in ilium MSCs. The mRNA levels of the seven similarly expressed genes were also compared with those in fibroblasts, which have little or no osteogenic differentiation potential. Among the seven genes, the mRNA levels of <i>IGF1</i> and <i>SRGN</i> in all MSCs examined were higher than those in any of the fibroblasts. These results suggest that measuring the mRNA levels of <i>IGF1</i> and <i>SRGN</i> before osteogenic induction will provide useful information for selecting competent MSCs for regenerative medicine, although the effectiveness of the markers is needed to be confirmed using a large number of MSCs, which have various levels of osteogenic differentiation potential. |
| first_indexed | 2024-03-10T04:23:54Z |
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| language | English |
| last_indexed | 2024-03-10T04:23:54Z |
| publishDate | 2021-11-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-66b7883799bd40aeb929c9c1d4f7a3fc2023-11-23T07:44:22ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-11-014332157216610.3390/cimb43030150The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal CellsMasami Kanawa0Akira Igarashi1Katsumi Fujimoto2Tania Saskianti3Ayumu Nakashima4Yukihito Higashi5Hidemi Kurihara6Yukio Kato7Takeshi Kawamoto8Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima 734-8533, JapanDivision of Advanced Technology and Development, BML, Inc., Saitama 350-1101, JapanDepartment of Dental and Medical Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanDepartment of Dental and Medical Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanDepartment of Stem Cell Biology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanResearch Center for Radiation Genome Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8533, JapanDepartment of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanDepartment of Dental and Medical Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanDepartment of Dental and Medical Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8533, JapanMesenchymal stromal cells (MSCs) have the potential to differentiate into a variety of mature cell types and are a promising source of regenerative medicine. The success of regenerative medicine using MSCs strongly depends on their differentiation potential. In this study, we sought to identify marker genes for predicting the osteogenic differentiation potential by comparing ilium MSC and fibroblast samples. We measured the mRNA levels of 95 candidate genes in nine ilium MSC and four fibroblast samples before osteogenic induction, and compared them with alkaline phosphatase (ALP) activity as a marker of osteogenic differentiation after induction. We identified 17 genes whose mRNA expression levels positively correlated with ALP activity. The chondrogenic and adipogenic differentiation potentials of jaw MSCs are much lower than those of ilium MSCs, although the osteogenic differentiation potential of jaw MSCs is comparable with that of ilium MSCs. To select markers suitable for predicting the osteogenic differentiation potential, we compared the mRNA levels of the 17 genes in ilium MSCs with those in jaw MSCs. The levels of 7 out of the 17 genes were not substantially different between the jaw and ilium MSCs, while the remaining 10 genes were expressed at significantly lower levels in jaw MSCs than in ilium MSCs. The mRNA levels of the seven similarly expressed genes were also compared with those in fibroblasts, which have little or no osteogenic differentiation potential. Among the seven genes, the mRNA levels of <i>IGF1</i> and <i>SRGN</i> in all MSCs examined were higher than those in any of the fibroblasts. These results suggest that measuring the mRNA levels of <i>IGF1</i> and <i>SRGN</i> before osteogenic induction will provide useful information for selecting competent MSCs for regenerative medicine, although the effectiveness of the markers is needed to be confirmed using a large number of MSCs, which have various levels of osteogenic differentiation potential.https://www.mdpi.com/1467-3045/43/3/150mesenchymal stromal cellosteogenesisfibroblastpredictive markerALP activityregenerative medicine |
| spellingShingle | Masami Kanawa Akira Igarashi Katsumi Fujimoto Tania Saskianti Ayumu Nakashima Yukihito Higashi Hidemi Kurihara Yukio Kato Takeshi Kawamoto The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells Current Issues in Molecular Biology mesenchymal stromal cell osteogenesis fibroblast predictive marker ALP activity regenerative medicine |
| title | The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells |
| title_full | The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells |
| title_fullStr | The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells |
| title_full_unstemmed | The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells |
| title_short | The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells |
| title_sort | identification of marker genes for predicting the osteogenic differentiation potential of mesenchymal stromal cells |
| topic | mesenchymal stromal cell osteogenesis fibroblast predictive marker ALP activity regenerative medicine |
| url | https://www.mdpi.com/1467-3045/43/3/150 |
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