Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers
Summary: Germinal centers (GCs) are sites where plasma and memory B cells form to generate high-affinity, Ig class-switched antibodies. Specialized stromal cells called follicular dendritic cells (FDCs) are essential for GC formation. During systemic Salmonella Typhimurium (STm) infection GCs are ab...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223003875 |
| _version_ | 1797869689551978496 |
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| author | Edith Marcial-Juárez Marisol Pérez-Toledo Saba Nayar Elena Pipi Areej Alshayea Ruby Persaud Sian E. Jossi Rachel Lamerton Francesca Barone Ian R. Henderson Adam F. Cunningham |
| author_facet | Edith Marcial-Juárez Marisol Pérez-Toledo Saba Nayar Elena Pipi Areej Alshayea Ruby Persaud Sian E. Jossi Rachel Lamerton Francesca Barone Ian R. Henderson Adam F. Cunningham |
| author_sort | Edith Marcial-Juárez |
| collection | DOAJ |
| description | Summary: Germinal centers (GCs) are sites where plasma and memory B cells form to generate high-affinity, Ig class-switched antibodies. Specialized stromal cells called follicular dendritic cells (FDCs) are essential for GC formation. During systemic Salmonella Typhimurium (STm) infection GCs are absent, whereas extensive extrafollicular and switched antibody responses are maintained. The mechanisms that underpin the absence of GC formation are incompletely understood. Here, we demonstrate that STm induces a reversible disruption of niches within the splenic microenvironment, including the T and B cell compartments and the marginal zone. Alongside these effects after infection, mature FDC networks are strikingly absent, whereas immature FDC precursors, including marginal sinus pre-FDCs (MadCAM-1+) and perivascular pre-FDCs (PDGFRβ+) are enriched. As normal FDC networks re-establish, extensive GCs become detectable throughout the spleen. Therefore, the reorganization of FDC networks and the loss of GC responses are key, parallel features of systemic STm infections. |
| first_indexed | 2024-04-10T00:16:32Z |
| format | Article |
| id | doaj.art-66b78e952b064cdaa6f3dbdd10691c6c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T00:16:32Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-66b78e952b064cdaa6f3dbdd10691c6c2023-03-16T05:06:00ZengElsevieriScience2589-00422023-04-01264106310Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centersEdith Marcial-Juárez0Marisol Pérez-Toledo1Saba Nayar2Elena Pipi3Areej Alshayea4Ruby Persaud5Sian E. Jossi6Rachel Lamerton7Francesca Barone8Ian R. Henderson9Adam F. Cunningham10Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United Kingdom; Corresponding authorInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomRheumatology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomRheumatology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United KingdomRheumatology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, West Midlands, B15 2TT, United Kingdom; National Institute for Health Research (NIHR) Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust, UK and Sandwell and West Birmingham Trust, Birmingham, West Midlands, B15 2TH, United KingdomInstitute for Molecular Bioscience, University of Queensland, Brisbane, QLD4072, AustraliaInstitute of Immunology and Immunotherapy, University of Birmingham, Birmingham, West Midlands, B15 2TT, United Kingdom; Corresponding authorSummary: Germinal centers (GCs) are sites where plasma and memory B cells form to generate high-affinity, Ig class-switched antibodies. Specialized stromal cells called follicular dendritic cells (FDCs) are essential for GC formation. During systemic Salmonella Typhimurium (STm) infection GCs are absent, whereas extensive extrafollicular and switched antibody responses are maintained. The mechanisms that underpin the absence of GC formation are incompletely understood. Here, we demonstrate that STm induces a reversible disruption of niches within the splenic microenvironment, including the T and B cell compartments and the marginal zone. Alongside these effects after infection, mature FDC networks are strikingly absent, whereas immature FDC precursors, including marginal sinus pre-FDCs (MadCAM-1+) and perivascular pre-FDCs (PDGFRβ+) are enriched. As normal FDC networks re-establish, extensive GCs become detectable throughout the spleen. Therefore, the reorganization of FDC networks and the loss of GC responses are key, parallel features of systemic STm infections.http://www.sciencedirect.com/science/article/pii/S2589004223003875ImmunologyImmune responseComponents of the immune systemMicrobiologyCell biology |
| spellingShingle | Edith Marcial-Juárez Marisol Pérez-Toledo Saba Nayar Elena Pipi Areej Alshayea Ruby Persaud Sian E. Jossi Rachel Lamerton Francesca Barone Ian R. Henderson Adam F. Cunningham Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers iScience Immunology Immune response Components of the immune system Microbiology Cell biology |
| title | Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| title_full | Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| title_fullStr | Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| title_full_unstemmed | Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| title_short | Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| title_sort | salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers |
| topic | Immunology Immune response Components of the immune system Microbiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223003875 |
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