Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria
Identification and manipulation of cellular energy regulation mechanisms may be a strategy to increase productivity in photosynthetic organisms. This work tests the hypothesis that polyphosphate synthesis and degradation play a role in energy management by storing or dissipating energy in the form o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1342496/full |
| _version_ | 1797322425080217600 |
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| author | Jacob Sebesta Michael Cantrell Eric Schaedig Harvey J. M. Hou Harvey J. M. Hou Colleen Pastore Katherine J. Chou Wei Xiong Michael T. Guarnieri Jianping Yu |
| author_facet | Jacob Sebesta Michael Cantrell Eric Schaedig Harvey J. M. Hou Harvey J. M. Hou Colleen Pastore Katherine J. Chou Wei Xiong Michael T. Guarnieri Jianping Yu |
| author_sort | Jacob Sebesta |
| collection | DOAJ |
| description | Identification and manipulation of cellular energy regulation mechanisms may be a strategy to increase productivity in photosynthetic organisms. This work tests the hypothesis that polyphosphate synthesis and degradation play a role in energy management by storing or dissipating energy in the form of ATP. A polyphosphate kinase (ppk) knock-out strain unable to synthesize polyphosphate was generated in the cyanobacterium Synechocystis sp. PCC 6803. This mutant strain demonstrated higher ATP levels and faster growth than the wildtype strain in high-carbon conditions and had a growth defect under multiple stress conditions. In a strain that combined ppk deletion with heterologous expression of ethylene-forming enzyme, higher ethylene productivity was observed than in the wildtype background. These results support the role of polyphosphate synthesis and degradation as an energy regulation mechanism and suggest that such mechanisms may be effective targets in biocontainment design. |
| first_indexed | 2024-03-08T05:13:10Z |
| format | Article |
| id | doaj.art-66b902e2ca114f89ab1f97dbad3dd1f8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T05:13:10Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-66b902e2ca114f89ab1f97dbad3dd1f82024-02-07T05:24:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13424961342496Polyphosphate kinase deletion increases laboratory productivity in cyanobacteriaJacob Sebesta0Michael Cantrell1Eric Schaedig2Harvey J. M. Hou3Harvey J. M. Hou4Colleen Pastore5Katherine J. Chou6Wei Xiong7Michael T. Guarnieri8Jianping Yu9Biosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesLaboratory of Forensic Analysis and Photosynthesis, Department of Physical Sciences, Alabama State University, Montgomery, AL, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesIdentification and manipulation of cellular energy regulation mechanisms may be a strategy to increase productivity in photosynthetic organisms. This work tests the hypothesis that polyphosphate synthesis and degradation play a role in energy management by storing or dissipating energy in the form of ATP. A polyphosphate kinase (ppk) knock-out strain unable to synthesize polyphosphate was generated in the cyanobacterium Synechocystis sp. PCC 6803. This mutant strain demonstrated higher ATP levels and faster growth than the wildtype strain in high-carbon conditions and had a growth defect under multiple stress conditions. In a strain that combined ppk deletion with heterologous expression of ethylene-forming enzyme, higher ethylene productivity was observed than in the wildtype background. These results support the role of polyphosphate synthesis and degradation as an energy regulation mechanism and suggest that such mechanisms may be effective targets in biocontainment design.https://www.frontiersin.org/articles/10.3389/fpls.2024.1342496/fullcyanobacteriaenergy regulationpolyphosphatebiocontainmentethylene |
| spellingShingle | Jacob Sebesta Michael Cantrell Eric Schaedig Harvey J. M. Hou Harvey J. M. Hou Colleen Pastore Katherine J. Chou Wei Xiong Michael T. Guarnieri Jianping Yu Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria Frontiers in Plant Science cyanobacteria energy regulation polyphosphate biocontainment ethylene |
| title | Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| title_full | Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| title_fullStr | Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| title_full_unstemmed | Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| title_short | Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| title_sort | polyphosphate kinase deletion increases laboratory productivity in cyanobacteria |
| topic | cyanobacteria energy regulation polyphosphate biocontainment ethylene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1342496/full |
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