AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator

In this communication, we present the prototype of a new simulated in situ lab/on-deck incubator, the light spectrum replicator (LSR), and a method for simulating the measured in situ HOCR light spectrum curves in incubation chambers. We developed this system using AI and genetic algorithms in an it...

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Main Authors: Staša Puškarić, Mateo Sokač, Živana Ninčević, Heliodor Prelesnik, Knut Yngve Børsheim
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Journal of Marine Science and Engineering
Subjects:
Online Access:https://www.mdpi.com/2077-1312/12/2/339
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author Staša Puškarić
Mateo Sokač
Živana Ninčević
Heliodor Prelesnik
Knut Yngve Børsheim
author_facet Staša Puškarić
Mateo Sokač
Živana Ninčević
Heliodor Prelesnik
Knut Yngve Børsheim
author_sort Staša Puškarić
collection DOAJ
description In this communication, we present the prototype of a new simulated in situ lab/on-deck incubator, the light spectrum replicator (LSR), and a method for simulating the measured in situ HOCR light spectrum curves in incubation chambers. We developed this system using AI and genetic algorithms in an iterative fashion to find the best-fitting light spectrum in situ irradiance at different depths. The HOCR light spectrum measured at the depth and time of sampling was processed immediately, so the incubator is in a stable and ready condition by the time the samples inoculated with <sup>14</sup>C were placed in sample holders (10 min after sampling). This incubator is intended to provide a reliable, fast, and easy-to-use tool for studying primary production based on the evaluation of the photosynthetic uptake of <sup>14</sup>C. This system enables short incubation periods for small samples: we tested incubations of 5 mL samples during 15 min incubation periods. Our initial measurements taken using the prototype revealed a sufficiently good correlation between the on-deck measurements and in situ incubations. This prototype can be improved, as discussed in this text.
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spelling doaj.art-66b9db438e5345308087bd67133805272024-02-23T15:23:20ZengMDPI AGJournal of Marine Science and Engineering2077-13122024-02-0112233910.3390/jmse12020339AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck IncubatorStaša Puškarić0Mateo Sokač1Živana Ninčević2Heliodor Prelesnik3Knut Yngve Børsheim4MARINIX Ocean Tech AS, 4617 Kristiansand, NorwayMARINIX Ocean Tech AS, 4617 Kristiansand, NorwayInstitute of Oceanography and Fisheries (IZOR), 21000 Split, CroatiaInstitute of Oceanography and Fisheries (IZOR), 21000 Split, CroatiaInstitute of Marine Research, 5005 Bergen, NorwayIn this communication, we present the prototype of a new simulated in situ lab/on-deck incubator, the light spectrum replicator (LSR), and a method for simulating the measured in situ HOCR light spectrum curves in incubation chambers. We developed this system using AI and genetic algorithms in an iterative fashion to find the best-fitting light spectrum in situ irradiance at different depths. The HOCR light spectrum measured at the depth and time of sampling was processed immediately, so the incubator is in a stable and ready condition by the time the samples inoculated with <sup>14</sup>C were placed in sample holders (10 min after sampling). This incubator is intended to provide a reliable, fast, and easy-to-use tool for studying primary production based on the evaluation of the photosynthetic uptake of <sup>14</sup>C. This system enables short incubation periods for small samples: we tested incubations of 5 mL samples during 15 min incubation periods. Our initial measurements taken using the prototype revealed a sufficiently good correlation between the on-deck measurements and in situ incubations. This prototype can be improved, as discussed in this text.https://www.mdpi.com/2077-1312/12/2/339primary productivityoceansimulated in situ incubatormethodirradiance
spellingShingle Staša Puškarić
Mateo Sokač
Živana Ninčević
Heliodor Prelesnik
Knut Yngve Børsheim
AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
Journal of Marine Science and Engineering
primary productivity
ocean
simulated in situ incubator
method
irradiance
title AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
title_full AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
title_fullStr AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
title_full_unstemmed AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
title_short AI-Light Spectrum Replicator (LSR): A Novel Simulated In Situ Lab/On-Deck Incubator
title_sort ai light spectrum replicator lsr a novel simulated in situ lab on deck incubator
topic primary productivity
ocean
simulated in situ incubator
method
irradiance
url https://www.mdpi.com/2077-1312/12/2/339
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