HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells
Due to a common mode of transmission through infected human blood, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection is relatively prevalent. In alignment with this, HCV co-infection is associated with an increased size of the HIV reservoir in highly active antiretroviral t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/13/2/134 |
| _version_ | 1797297269981052928 |
|---|---|
| author | Cinthya Alicia Marcela López Rosa Nicole Freiberger Franco Agustín Sviercz Jorge Quarleri María Victoria Delpino |
| author_facet | Cinthya Alicia Marcela López Rosa Nicole Freiberger Franco Agustín Sviercz Jorge Quarleri María Victoria Delpino |
| author_sort | Cinthya Alicia Marcela López |
| collection | DOAJ |
| description | Due to a common mode of transmission through infected human blood, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection is relatively prevalent. In alignment with this, HCV co-infection is associated with an increased size of the HIV reservoir in highly active antiretroviral therapy (HAART)-treated individuals. Hence, it is crucial to comprehend the physiological mechanisms governing the latency and reactivation of HIV in reservoirs. Consequently, our study delves into the interplay between HCV/HIV co-infection in liver cells and its impact on the modulation of HIV latency. We utilized the latently infected monocytic cell line (U1) and the latently infected T-cell line (J-Lat) and found that mediators produced by the infection of hepatic stellate cells and hepatocytes with HIV and HCV, respectively, were incapable of inducing latency reversal under the studied conditions. This may favor the maintenance of the HIV reservoir size among latently infected mononuclear cells in the liver. Further investigations are essential to elucidate the role of the interaction between liver cells in regulating HIV latency and/or reactivation, providing a physiologically relevant model for comprehending reservoir microenvironments in vivo. |
| first_indexed | 2024-03-07T22:18:41Z |
| format | Article |
| id | doaj.art-66bb045bbf6744a78bd23e370328895c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-07T22:18:41Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-66bb045bbf6744a78bd23e370328895c2024-02-23T15:30:18ZengMDPI AGPathogens2076-08172024-02-0113213410.3390/pathogens13020134HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear CellsCinthya Alicia Marcela López0Rosa Nicole Freiberger1Franco Agustín Sviercz2Jorge Quarleri3María Victoria Delpino4Instituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Consejo de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires 1121, ArgentinaInstituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Consejo de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires 1121, ArgentinaInstituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Consejo de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires 1121, ArgentinaInstituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Consejo de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires 1121, ArgentinaInstituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Consejo de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires 1121, ArgentinaDue to a common mode of transmission through infected human blood, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection is relatively prevalent. In alignment with this, HCV co-infection is associated with an increased size of the HIV reservoir in highly active antiretroviral therapy (HAART)-treated individuals. Hence, it is crucial to comprehend the physiological mechanisms governing the latency and reactivation of HIV in reservoirs. Consequently, our study delves into the interplay between HCV/HIV co-infection in liver cells and its impact on the modulation of HIV latency. We utilized the latently infected monocytic cell line (U1) and the latently infected T-cell line (J-Lat) and found that mediators produced by the infection of hepatic stellate cells and hepatocytes with HIV and HCV, respectively, were incapable of inducing latency reversal under the studied conditions. This may favor the maintenance of the HIV reservoir size among latently infected mononuclear cells in the liver. Further investigations are essential to elucidate the role of the interaction between liver cells in regulating HIV latency and/or reactivation, providing a physiologically relevant model for comprehending reservoir microenvironments in vivo.https://www.mdpi.com/2076-0817/13/2/134HIVHCVco-infectionJ-LatU1latency reversal |
| spellingShingle | Cinthya Alicia Marcela López Rosa Nicole Freiberger Franco Agustín Sviercz Jorge Quarleri María Victoria Delpino HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells Pathogens HIV HCV co-infection J-Lat U1 latency reversal |
| title | HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells |
| title_full | HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells |
| title_fullStr | HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells |
| title_full_unstemmed | HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells |
| title_short | HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells |
| title_sort | hiv infected hepatic stellate cells or hcv infected hepatocytes are unable to promote latency reversal among hiv infected mononuclear cells |
| topic | HIV HCV co-infection J-Lat U1 latency reversal |
| url | https://www.mdpi.com/2076-0817/13/2/134 |
| work_keys_str_mv | AT cinthyaaliciamarcelalopez hivinfectedhepaticstellatecellsorhcvinfectedhepatocytesareunabletopromotelatencyreversalamonghivinfectedmononuclearcells AT rosanicolefreiberger hivinfectedhepaticstellatecellsorhcvinfectedhepatocytesareunabletopromotelatencyreversalamonghivinfectedmononuclearcells AT francoagustinsviercz hivinfectedhepaticstellatecellsorhcvinfectedhepatocytesareunabletopromotelatencyreversalamonghivinfectedmononuclearcells AT jorgequarleri hivinfectedhepaticstellatecellsorhcvinfectedhepatocytesareunabletopromotelatencyreversalamonghivinfectedmononuclearcells AT mariavictoriadelpino hivinfectedhepaticstellatecellsorhcvinfectedhepatocytesareunabletopromotelatencyreversalamonghivinfectedmononuclearcells |