Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing
IntroductionHuman papilloma virus (HPV) is the most common sexually transmitted infection worldwide. Cervicovaginal microbiota plays an important role in HPV infection and is associated with the development of squamous intraepithelial lesions (SIL). The natural history of cervical cancer involves re...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1334502/full |
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| author | Anastasiya Peremykina Valery Cheranev Andrey Krivoy Alexander O. Andreev Zhanna Repinskaia Aleksandra V. Asaturova Dmitriy Korostin Denis Rebrikov Gyuldana R. Bayramova |
| author_facet | Anastasiya Peremykina Valery Cheranev Andrey Krivoy Alexander O. Andreev Zhanna Repinskaia Aleksandra V. Asaturova Dmitriy Korostin Denis Rebrikov Gyuldana R. Bayramova |
| author_sort | Anastasiya Peremykina |
| collection | DOAJ |
| description | IntroductionHuman papilloma virus (HPV) is the most common sexually transmitted infection worldwide. Cervicovaginal microbiota plays an important role in HPV infection and is associated with the development of squamous intraepithelial lesions (SIL). The natural history of cervical cancer involves reversible changes in the cervical tissue from a normal state, in which no neoplastic changes are detected in the squamous epithelium, to varying states of cellular abnormalities that ultimately lead to cervical cancer. Low-grade SIL (LSIL), like another cytological category - atypical squamous cells of undetermined significance (ASCUS), may progress to high-grade SIL (HSIL) and invasive cervical cancer or may regress to a normal state.MethodsIn this work, we studied cervical canal microbiome in 165 HPV-positive and HPV-negative women of a reproductive age with ASCUS [HPV(+) n = 29; HPV(−) n = 11], LSIL [HPV(+) n = 32; HPV(−) n = 25], HSIL [HPV(+) n = 46], and the control group with negative for intraepithelial lesion malignancy (NILM) [HPV(−) n = 22].Results and DiscussionHPV16 is the most prevalent HPV type. We have not found any differences between diversity in studied groups, but several genus [like Prevotella (p-value = 0.026), Gardnerella (p-value = 0.003), Fannyhessea (p-value = 0.024)] more often occurred in HSIL group compared by NILM or LSIL regardless of HPV. We have found statistically significant difference in occurrence or proportion of bacterial genus in studied groups. We also identified that increasing of the ratio of Lactobacillus iners or age of patient lead to higher chance to HSIL, while increasing of the ratio of Lactobacillus crispatus lead to higher chance to LSIL. Patients with a moderate dysbiosis equally often had either of three types of vaginal microbial communities (CST, Community State Type) with the prevalence of Lactobacillus crispatus (CST I), Lactobacillus gasseri (CST II), and Lactobacillus iners (CST III); whereas severe dysbiosis is linked with CST IV involving the microorganisms genera associated with bacterial vaginosis and aerobic vaginitis: Gardnerella, Fannyhessea, Dialister, Sneathia, Anaerococcus, Megasphaera, Prevotella, Finegoldia, Peptoniphilus, Porphyromonas, Parvimonas, and Streptococcus. |
| first_indexed | 2024-04-25T00:06:56Z |
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| language | English |
| last_indexed | 2024-04-25T00:06:56Z |
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| spelling | doaj.art-66bba3b41ef1487294790383a2e156e42024-03-14T05:09:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13345021334502Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencingAnastasiya Peremykina0Valery Cheranev1Andrey Krivoy2Alexander O. Andreev3Zhanna Repinskaia4Aleksandra V. Asaturova5Dmitriy Korostin6Denis Rebrikov7Gyuldana R. Bayramova8Department of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Ministry of Health of the Russian Federation, Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, RussiaDepartment of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Ministry of Health of the Russian Federation, Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, RussiaDepartment of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Ministry of Health of the Russian Federation, Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, RussiaDepartment of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Ministry of Health of the Russian Federation, Moscow, RussiaIntroductionHuman papilloma virus (HPV) is the most common sexually transmitted infection worldwide. Cervicovaginal microbiota plays an important role in HPV infection and is associated with the development of squamous intraepithelial lesions (SIL). The natural history of cervical cancer involves reversible changes in the cervical tissue from a normal state, in which no neoplastic changes are detected in the squamous epithelium, to varying states of cellular abnormalities that ultimately lead to cervical cancer. Low-grade SIL (LSIL), like another cytological category - atypical squamous cells of undetermined significance (ASCUS), may progress to high-grade SIL (HSIL) and invasive cervical cancer or may regress to a normal state.MethodsIn this work, we studied cervical canal microbiome in 165 HPV-positive and HPV-negative women of a reproductive age with ASCUS [HPV(+) n = 29; HPV(−) n = 11], LSIL [HPV(+) n = 32; HPV(−) n = 25], HSIL [HPV(+) n = 46], and the control group with negative for intraepithelial lesion malignancy (NILM) [HPV(−) n = 22].Results and DiscussionHPV16 is the most prevalent HPV type. We have not found any differences between diversity in studied groups, but several genus [like Prevotella (p-value = 0.026), Gardnerella (p-value = 0.003), Fannyhessea (p-value = 0.024)] more often occurred in HSIL group compared by NILM or LSIL regardless of HPV. We have found statistically significant difference in occurrence or proportion of bacterial genus in studied groups. We also identified that increasing of the ratio of Lactobacillus iners or age of patient lead to higher chance to HSIL, while increasing of the ratio of Lactobacillus crispatus lead to higher chance to LSIL. Patients with a moderate dysbiosis equally often had either of three types of vaginal microbial communities (CST, Community State Type) with the prevalence of Lactobacillus crispatus (CST I), Lactobacillus gasseri (CST II), and Lactobacillus iners (CST III); whereas severe dysbiosis is linked with CST IV involving the microorganisms genera associated with bacterial vaginosis and aerobic vaginitis: Gardnerella, Fannyhessea, Dialister, Sneathia, Anaerococcus, Megasphaera, Prevotella, Finegoldia, Peptoniphilus, Porphyromonas, Parvimonas, and Streptococcus.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1334502/fullsquamous intraepithelial lesion16S rRNA gene sequencingHPVcervicovaginal microbiotaSIL prediction |
| spellingShingle | Anastasiya Peremykina Valery Cheranev Andrey Krivoy Alexander O. Andreev Zhanna Repinskaia Aleksandra V. Asaturova Dmitriy Korostin Denis Rebrikov Gyuldana R. Bayramova Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing Frontiers in Microbiology squamous intraepithelial lesion 16S rRNA gene sequencing HPV cervicovaginal microbiota SIL prediction |
| title | Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing |
| title_full | Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing |
| title_fullStr | Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing |
| title_full_unstemmed | Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing |
| title_short | Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing |
| title_sort | microbiome markers in hpv positive and hpv negative women of reproductive age with ascus and sil determined by v4 region of 16s rrna gene sequencing |
| topic | squamous intraepithelial lesion 16S rRNA gene sequencing HPV cervicovaginal microbiota SIL prediction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1334502/full |
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