Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells
DNA damage usually happens due to the several chemical materials that induce genotoxic effect in normal cells. Cinnamon essential oil (CEO), which contains cinnamaldehyde as its major compound, has been reported to possess antioxidant activity to prevent DNA damage. The aim of this study is to evalu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2017-04-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/122 |
| _version_ | 1811231729851039744 |
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| author | Layung Sekar Sih Wikanthi Nindi Wulandari Yuni Fajar Esti Nur Fitra Sari Ratna Asmah Susidarti |
| author_facet | Layung Sekar Sih Wikanthi Nindi Wulandari Yuni Fajar Esti Nur Fitra Sari Ratna Asmah Susidarti |
| author_sort | Layung Sekar Sih Wikanthi |
| collection | DOAJ |
| description | DNA damage usually happens due to the several chemical materials that induce genotoxic effect in normal cells. Cinnamon essential oil (CEO), which contains cinnamaldehyde as its major compound, has been reported to possess antioxidant activity to prevent DNA damage. The aim of this study is to evaluate the genotoxic and cytotoxic effect of CEO on doxorubicin-induced Chinese Hamster Ovary (CHO-K1) cells. The cytotoxic effect of CEO was determined by MTT assay with the parameter of IC50 while the genotoxic effect was carried out by micronucleus (MN) assay by using acridine orange fluorescent staining with the parameter of MN/1000 cells reduction number. Based on MTT assay, CEO showed cytotoxic activity with the IC50 value of 30 μg/ml and for MN assay, 3 μg/ml (1/10 IC50) of CEO decreased the percentage of micronucleus per 1000 cells up to 94,55%. Thus, the result can be summarized that CEO does not induce genotoxic and has the potency to prevent DNA damage caused by doxorubicin on CHO-K1 cells.
Keywords: genotoxic, cinnamomum essential oil (CEO), micronuclei assay, in vitro |
| first_indexed | 2024-04-12T10:49:59Z |
| format | Article |
| id | doaj.art-66bd0594b0d346a2a0ad8a40c6f5d1a8 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-12T10:49:59Z |
| publishDate | 2017-04-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-66bd0594b0d346a2a0ad8a40c6f5d1a82022-12-22T03:36:16ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892017-04-0181273110.14499/indonesianjcanchemoprev8iss1pp27-31118Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 CellsLayung Sekar Sih Wikanthi0Nindi Wulandari1Yuni Fajar Esti2Nur Fitra Sari3Ratna Asmah Susidarti4Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDNA damage usually happens due to the several chemical materials that induce genotoxic effect in normal cells. Cinnamon essential oil (CEO), which contains cinnamaldehyde as its major compound, has been reported to possess antioxidant activity to prevent DNA damage. The aim of this study is to evaluate the genotoxic and cytotoxic effect of CEO on doxorubicin-induced Chinese Hamster Ovary (CHO-K1) cells. The cytotoxic effect of CEO was determined by MTT assay with the parameter of IC50 while the genotoxic effect was carried out by micronucleus (MN) assay by using acridine orange fluorescent staining with the parameter of MN/1000 cells reduction number. Based on MTT assay, CEO showed cytotoxic activity with the IC50 value of 30 μg/ml and for MN assay, 3 μg/ml (1/10 IC50) of CEO decreased the percentage of micronucleus per 1000 cells up to 94,55%. Thus, the result can be summarized that CEO does not induce genotoxic and has the potency to prevent DNA damage caused by doxorubicin on CHO-K1 cells. Keywords: genotoxic, cinnamomum essential oil (CEO), micronuclei assay, in vitrohttps://ijcc.chemoprev.org/index.php/ijcc/article/view/122 |
| spellingShingle | Layung Sekar Sih Wikanthi Nindi Wulandari Yuni Fajar Esti Nur Fitra Sari Ratna Asmah Susidarti Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells |
| title_full | Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells |
| title_fullStr | Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells |
| title_full_unstemmed | Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells |
| title_short | Cinnamomum Essential Oil Prevents DNA Damage-Induced by Doxorubicin on CHO-K1 Cells |
| title_sort | cinnamomum essential oil prevents dna damage induced by doxorubicin on cho k1 cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/122 |
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