Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption
Indospicine, a hepatotoxic arginine analog, occurs in leguminous plants of the Indigofera genus and accumulates in the tissues of grazing animals that consume these plants. Furthermore, indospicine has caused toxicity in dogs following consumption of indospicine-contaminated meat; however, the poten...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/9/356 |
| _version_ | 1811187388337094656 |
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| author | Saira Sultan Cindy Giles Gabriele Netzel Simone A. Osborne Michael E. Netzel Mary T. Fletcher |
| author_facet | Saira Sultan Cindy Giles Gabriele Netzel Simone A. Osborne Michael E. Netzel Mary T. Fletcher |
| author_sort | Saira Sultan |
| collection | DOAJ |
| description | Indospicine, a hepatotoxic arginine analog, occurs in leguminous plants of the Indigofera genus and accumulates in the tissues of grazing animals that consume these plants. Furthermore, indospicine has caused toxicity in dogs following consumption of indospicine-contaminated meat; however, the potential impact on human health is unknown. The present study was designed to determine the effect of simulated human gastrointestinal digestion on the release and degradation of indospicine from contaminated camel meat following microwave cooking. Results showed no significant (p > 0.05) indospicine degradation during cooking or in vitro digestion. However, approximately 70% indospicine was released from the meat matrix into the liquid digesta during the gastric phase (in the presence of pepsin) and increased to >90% in the intestinal phase (with pancreatic enzymes). Following human consumption of contaminated meat, this soluble and more bioaccessible fraction of intact indospicine could be readily available for absorption by the small intestine, potentially circulating indospicine throughout the human body to tissues where it could accumulate and cause detrimental toxic effects. |
| first_indexed | 2024-04-11T14:02:36Z |
| format | Article |
| id | doaj.art-66c692f9b30c4ce2ba7e8dcdc0c75357 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T14:02:36Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-66c692f9b30c4ce2ba7e8dcdc0c753572022-12-22T04:20:04ZengMDPI AGToxins2072-66512018-09-0110935610.3390/toxins10090356toxins10090356Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human ConsumptionSaira Sultan0Cindy Giles1Gabriele Netzel2Simone A. Osborne3Michael E. Netzel4Mary T. Fletcher5Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, AustraliaDepartment of Agriculture and Fisheries, Queensland Government, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, AustraliaCommonwealth Scientific and Industrial Research Organisation, Agriculture and Food, St. Lucia, QLD 4067, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, AustraliaIndospicine, a hepatotoxic arginine analog, occurs in leguminous plants of the Indigofera genus and accumulates in the tissues of grazing animals that consume these plants. Furthermore, indospicine has caused toxicity in dogs following consumption of indospicine-contaminated meat; however, the potential impact on human health is unknown. The present study was designed to determine the effect of simulated human gastrointestinal digestion on the release and degradation of indospicine from contaminated camel meat following microwave cooking. Results showed no significant (p > 0.05) indospicine degradation during cooking or in vitro digestion. However, approximately 70% indospicine was released from the meat matrix into the liquid digesta during the gastric phase (in the presence of pepsin) and increased to >90% in the intestinal phase (with pancreatic enzymes). Following human consumption of contaminated meat, this soluble and more bioaccessible fraction of intact indospicine could be readily available for absorption by the small intestine, potentially circulating indospicine throughout the human body to tissues where it could accumulate and cause detrimental toxic effects.http://www.mdpi.com/2072-6651/10/9/356indospicinehepatotoxicitymeatin vitro digestionhuman |
| spellingShingle | Saira Sultan Cindy Giles Gabriele Netzel Simone A. Osborne Michael E. Netzel Mary T. Fletcher Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption Toxins indospicine hepatotoxicity meat in vitro digestion human |
| title | Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption |
| title_full | Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption |
| title_fullStr | Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption |
| title_full_unstemmed | Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption |
| title_short | Release of Indospicine from Contaminated Camel Meat following Cooking and Simulated Gastrointestinal Digestion: Implications for Human Consumption |
| title_sort | release of indospicine from contaminated camel meat following cooking and simulated gastrointestinal digestion implications for human consumption |
| topic | indospicine hepatotoxicity meat in vitro digestion human |
| url | http://www.mdpi.com/2072-6651/10/9/356 |
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