Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)

In this study, we chemically treated apple tree chips and evaluated the colonisation of these substrates by the mycelium of Pleurotus. ostreatus. Chips were treated by a liquid surfactant Empigen OB (0.02 – 0.32 %), calcium hydroxide Ca(OH)2 (1 – 3 %) and sodium hypochlorite NaClO (0.5 – 1.5 %), and...

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Main Authors: Ivan Jablonský, Martin Koudela, David Novotný
Format: Article
Language:English
Published: Mendel University Press 2017-01-01
Series:Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
Subjects:
Online Access:https://acta.mendelu.cz/65/4/1175/
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author Ivan Jablonský
Martin Koudela
David Novotný
author_facet Ivan Jablonský
Martin Koudela
David Novotný
author_sort Ivan Jablonský
collection DOAJ
description In this study, we chemically treated apple tree chips and evaluated the colonisation of these substrates by the mycelium of Pleurotus. ostreatus. Chips were treated by a liquid surfactant Empigen OB (0.02 – 0.32 %), calcium hydroxide Ca(OH)2 (1 – 3 %) and sodium hypochlorite NaClO (0.5 – 1.5 %), and by their combinations. Substrates treated with a combination of the Empigen and NaClO reached the pH values of 6.5, with a combination of the Empigen and hydroxide, pH values did not exceed 7.9. Significantly higher gains of P. ostreatus mycelium 15 days after inoculation were found for the substrate treated with 2 % Ca(OH)2 and in the substrate treated with 0.08 % Empigen. The majority of substrates treated with the combination of NaClO and the Empigen showed significantly higher mycelium gains 15 days after inoculation compared to the control. The best results were obtained with the combination 0.08 % Empigen + 1.5 % NaClO. These results indicate positive synergic effect of the Empigen and NaClO. Collectively, treatment of the substrate based on apple tree chips by surfactant Empigen, Ca(OH)2 and NaClO (apart from the combination of the surfactant and Ca(OH)2 seem to be a suitable approach for substrate preparation.
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spelling doaj.art-66c94fd7d4544b529dbbb2d64863264f2022-12-22T00:35:31ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102017-01-016541175118110.11118/actaun201765041175Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)Ivan Jablonský0Martin Koudela1David Novotný2Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Horticulture, Kamýcká 129, 165 21 Prague, Czech RepublicCzech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Horticulture, Kamýcká 129, 165 21 Prague, Czech RepublicCrop Research Institute v.v.i, Prague, Drnovská 507/73, 161 06 Prague, Czech RepublicIn this study, we chemically treated apple tree chips and evaluated the colonisation of these substrates by the mycelium of Pleurotus. ostreatus. Chips were treated by a liquid surfactant Empigen OB (0.02 – 0.32 %), calcium hydroxide Ca(OH)2 (1 – 3 %) and sodium hypochlorite NaClO (0.5 – 1.5 %), and by their combinations. Substrates treated with a combination of the Empigen and NaClO reached the pH values of 6.5, with a combination of the Empigen and hydroxide, pH values did not exceed 7.9. Significantly higher gains of P. ostreatus mycelium 15 days after inoculation were found for the substrate treated with 2 % Ca(OH)2 and in the substrate treated with 0.08 % Empigen. The majority of substrates treated with the combination of NaClO and the Empigen showed significantly higher mycelium gains 15 days after inoculation compared to the control. The best results were obtained with the combination 0.08 % Empigen + 1.5 % NaClO. These results indicate positive synergic effect of the Empigen and NaClO. Collectively, treatment of the substrate based on apple tree chips by surfactant Empigen, Ca(OH)2 and NaClO (apart from the combination of the surfactant and Ca(OH)2 seem to be a suitable approach for substrate preparation.https://acta.mendelu.cz/65/4/1175/oyster mushroomapple tree chipsmyceliumsubstratesurfactantcalcium hydroxide
spellingShingle Ivan Jablonský
Martin Koudela
David Novotný
Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
oyster mushroom
apple tree chips
mycelium
substrate
surfactant
calcium hydroxide
title Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
title_full Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
title_fullStr Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
title_full_unstemmed Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
title_short Comparing Treatment Methods of Apple Tree Chips in Terms of Mycelia Growth of Oyster Mushroom (Pleurotus ostreatus (Jacq.) P. Kumm)
title_sort comparing treatment methods of apple tree chips in terms of mycelia growth of oyster mushroom pleurotus ostreatus jacq p kumm
topic oyster mushroom
apple tree chips
mycelium
substrate
surfactant
calcium hydroxide
url https://acta.mendelu.cz/65/4/1175/
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