Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>)
The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Applied Microbiology |
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| Online Access: | https://www.mdpi.com/2673-8007/3/4/77 |
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| author | Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal |
| author_facet | Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal |
| author_sort | Jennifer McCabe |
| collection | DOAJ |
| description | The discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel <i>Paenibacillus</i> sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf (<i>Canis lupus</i>). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of <i>Staphylococcus aureus, Escherichia coli</i> and <i>Micrococcus luteus</i>. The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related <i>Paenibacillus</i> spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated <i>Paenibacillus</i> sp. ClWae2A, could be a potential candidate probiotic for domestic dogs. |
| first_indexed | 2024-03-08T18:34:45Z |
| format | Article |
| id | doaj.art-66c9cb5c14a544e7ad51c59cbc79778b |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-03-08T18:34:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-66c9cb5c14a544e7ad51c59cbc79778b2023-12-29T15:46:04ZengMDPI AGApplied Microbiology2673-80072023-09-01341120112910.3390/applmicrobiol3040077Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>)Jennifer McCabe0Jessika L. Bryant1C. Cristoph Klews2MiCayla Johnson3Ariel N. Atchley4Thomas W. Cousins5Analiska Dominguez6Marie Gabriel7Katie Middleton8Natasha A. Bowles9Heather M. Broughton10Kristina M. Smith11Mark R. Ackermann12Robert Bildfell13Patrick N. Ball14Evan S. Forsythe15Bruce S. Seal16Biology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USAOregon Veterinary Diagnostic Laboratory, OSU Carlson College of Veterinary Medicine, 134 Magruder Hall, 700 SW 30th, Corvallis, OR 97331, USAOregon Veterinary Diagnostic Laboratory, OSU Carlson College of Veterinary Medicine, 134 Magruder Hall, 700 SW 30th, Corvallis, OR 97331, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USAThe discovery of novel probiotic bacteria from free-ranging animals for the treatment of inflammatory bowel disease in domestic pets is a unique approach. The chloroform extraction of gastrointestinal (GI) tract material was used to inactivate vegetative cells and select for spore-forming bacteria. A bacterium identified as a novel <i>Paenibacillus</i> sp. strain via small ribosomal RNA (16S) gene sequencing was isolated from the GI tract of a gray wolf (<i>Canis lupus</i>). The bacterium was typed as Gram-variable, both catalase/oxidase-positive and positive via starch hydrolysis and lipase assays. The bacterium inhibited the growth of <i>Staphylococcus aureus, Escherichia coli</i> and <i>Micrococcus luteus</i>. The draft whole genome sequence (WGS) assembly was 7,034,206 bp in length, encoding 6543 genes, and is similar in size and coding capacity to other closely related <i>Paenibacillus</i> spp. The isolate’s genome encodes several germination and sporulation gene products along with antimicrobials such as a bacteriocin system and chitinase. Enzyme genes such as alpha amylase, cellulase, lipases and pectin lyase are also present in the genome. An incomplete lysogenic bacteriophage genome was also present in the isolate’s genome. Phenotypic characteristics combined with a WGS genotype analysis indicate that this bacterium, designated <i>Paenibacillus</i> sp. ClWae2A, could be a potential candidate probiotic for domestic dogs.https://www.mdpi.com/2673-8007/3/4/77probioticcanine inflammatory bowel diseaseantimicrobialgenomics<i>Paenibacillaceae</i> |
| spellingShingle | Jennifer McCabe Jessika L. Bryant C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Analiska Dominguez Marie Gabriel Katie Middleton Natasha A. Bowles Heather M. Broughton Kristina M. Smith Mark R. Ackermann Robert Bildfell Patrick N. Ball Evan S. Forsythe Bruce S. Seal Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) Applied Microbiology probiotic canine inflammatory bowel disease antimicrobial genomics <i>Paenibacillaceae</i> |
| title | Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) |
| title_full | Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) |
| title_fullStr | Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) |
| title_full_unstemmed | Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) |
| title_short | Phenotypic and Draft Genome Sequence Analyses of a <i>Paenibacillus</i> sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (<i>Canis lupus</i>) |
| title_sort | phenotypic and draft genome sequence analyses of a i paenibacillus i sp isolated from the gastrointestinal tract of a north american gray wolf i canis lupus i |
| topic | probiotic canine inflammatory bowel disease antimicrobial genomics <i>Paenibacillaceae</i> |
| url | https://www.mdpi.com/2673-8007/3/4/77 |
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