Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments. Here, we review our research primarily applying...

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Bibliographic Details
Main Authors: Mohamed A. Sobhy, Muhammad Tehseen, Masateru Takahashi, Amer Bralić, Alfredo De Biasio, Samir M. Hamdan
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
DNA Replication
Flap endonuclease
DNA Polymerase δ
Single-molecule fluorescence
FRET
PIFE
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037021003196

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