In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal in Bronchial Epithelial Cells
Background and objective China’s Xuan Wei County in Yunnan Province have the world’s highest incidence of lung cancer in nonsmoking women-20 times higher than the rest of China. Previous studies showed, this high lung cancer incidence may be associated with the silica particles embedded in the produ...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2012-10-01
|
| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2012.10.01 |
| _version_ | 1811192271621586944 |
|---|---|
| author | Guangjian LI Yunchao HUANG Yongjun LIU Lv GUO Yongchun ZHOU Kun YANG Ying CHEN Guangqiang ZHAO Yujie LEI |
| author_facet | Guangjian LI Yunchao HUANG Yongjun LIU Lv GUO Yongchun ZHOU Kun YANG Ying CHEN Guangqiang ZHAO Yujie LEI |
| author_sort | Guangjian LI |
| collection | DOAJ |
| description | Background and objective China’s Xuan Wei County in Yunnan Province have the world’s highest incidence of lung cancer in nonsmoking women-20 times higher than the rest of China. Previous studies showed, this high lung cancer incidence may be associated with the silica particles embedded in the production combustion from the C1 coal. The aim of this study is to separate the silica particles from production combustion from the C1 bituminous coal in Xuan Wei County of Yunnan Province, and study in vitro toxicity of naturally occurring silica particles on BEAS-2B. Methods ①Separating the silica particles from combustion products of C1 bituminous coal by physical method, observing the morphology by Scanning Electron Microscope, analysis elements by SEM-EDX, observed the single particle morphology by Transmission Electron Microscope, analyed its particle size distribution by Laser particle size analyzer, the surface area of silica particles were determined by BET nitrogen adsorption analysis; ②Cell viability of the experimental group (silica; naturally occurring), control group (silica; industrial produced and crystalline silica) was detected by assay used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the reactive oxygen species (ROS), lactate dehydrogenase (LDH) were determined after 24 h-72 h exposed to these particles. Results ①The physical method can separate silica particles from production combustion from the C1 bituminous coal, which have different size, and from 30 nm to 120 nm particles accounted for 86.8%, different morphology, irregular surface area and containing trace of aluminum, calcium and iron and other elements; ②Under the same concentration, the experiment group have higher toxicity on BEAS-2B than control groups. Conclusion ①Physical method can separate silica particles from production combustion from the C1 bituminous coal and not change the original morphology and containing trace; ②Naturally occurring silica nanoparticles have irregular morphology, surface area, and containing complex trace elements may has greater toxicity than the silica nanoparticle of industrial produced and crystalline silica. |
| first_indexed | 2024-04-11T23:49:43Z |
| format | Article |
| id | doaj.art-66cd08b61e6c4f3baf5b13eed6f56fa4 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-11T23:49:43Z |
| publishDate | 2012-10-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-66cd08b61e6c4f3baf5b13eed6f56fa42022-12-22T03:56:31ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872012-10-01151056156810.3779/j.issn.1009-3419.2012.10.01In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial CellsGuangjian LIYunchao HUANGYongjun LIULv GUOYongchun ZHOUKun YANGYing CHENGuangqiang ZHAOYujie LEIBackground and objective China’s Xuan Wei County in Yunnan Province have the world’s highest incidence of lung cancer in nonsmoking women-20 times higher than the rest of China. Previous studies showed, this high lung cancer incidence may be associated with the silica particles embedded in the production combustion from the C1 coal. The aim of this study is to separate the silica particles from production combustion from the C1 bituminous coal in Xuan Wei County of Yunnan Province, and study in vitro toxicity of naturally occurring silica particles on BEAS-2B. Methods ①Separating the silica particles from combustion products of C1 bituminous coal by physical method, observing the morphology by Scanning Electron Microscope, analysis elements by SEM-EDX, observed the single particle morphology by Transmission Electron Microscope, analyed its particle size distribution by Laser particle size analyzer, the surface area of silica particles were determined by BET nitrogen adsorption analysis; ②Cell viability of the experimental group (silica; naturally occurring), control group (silica; industrial produced and crystalline silica) was detected by assay used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the reactive oxygen species (ROS), lactate dehydrogenase (LDH) were determined after 24 h-72 h exposed to these particles. Results ①The physical method can separate silica particles from production combustion from the C1 bituminous coal, which have different size, and from 30 nm to 120 nm particles accounted for 86.8%, different morphology, irregular surface area and containing trace of aluminum, calcium and iron and other elements; ②Under the same concentration, the experiment group have higher toxicity on BEAS-2B than control groups. Conclusion ①Physical method can separate silica particles from production combustion from the C1 bituminous coal and not change the original morphology and containing trace; ②Naturally occurring silica nanoparticles have irregular morphology, surface area, and containing complex trace elements may has greater toxicity than the silica nanoparticle of industrial produced and crystalline silica.http://dx.doi.org/10.3779/j.issn.1009-3419.2012.10.01C1 bituminous coalSilica nanoparticleSeparationMorphology characteristicsVitro toxicity |
| spellingShingle | Guangjian LI Yunchao HUANG Yongjun LIU Lv GUO Yongchun ZHOU Kun YANG Ying CHEN Guangqiang ZHAO Yujie LEI In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal in Bronchial Epithelial Cells Chinese Journal of Lung Cancer C1 bituminous coal Silica nanoparticle Separation Morphology characteristics Vitro toxicity |
| title | In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial Cells |
| title_full | In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial Cells |
| title_fullStr | In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial Cells |
| title_full_unstemmed | In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial Cells |
| title_short | In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal
in Bronchial Epithelial Cells |
| title_sort | in vitro toxicity of naturally occurring silica nanoparticles in c1 coal
in bronchial epithelial cells |
| topic | C1 bituminous coal Silica nanoparticle Separation Morphology characteristics Vitro toxicity |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2012.10.01 |
| work_keys_str_mv | AT guangjianli invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT yunchaohuang invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT yongjunliu invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT lvguo invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT yongchunzhou invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT kunyang invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT yingchen invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT guangqiangzhao invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells AT yujielei invitrotoxicityofnaturallyoccurringsilicananoparticlesinc1coalinbronchialepithelialcells |