Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression
Carbon monoxide (CO) has been proposed as a chemical light signal and neural system modulator via heme oxygenases -1 and -2 (HO-1 and HO-2). Many papers have proven the CO-HO circuit to be important for such physiological pathways as the molecular biological clock and the GnRH axis, but also in such...
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MDPI AG
2022-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/2/358 |
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| author | Sławomir Nowak Przemysław Gilun Katarzyna Kozioł Maria Romerowicz-Misielak Magdalena Koziorowska-Gilun Barbara Wąsowska |
| author_facet | Sławomir Nowak Przemysław Gilun Katarzyna Kozioł Maria Romerowicz-Misielak Magdalena Koziorowska-Gilun Barbara Wąsowska |
| author_sort | Sławomir Nowak |
| collection | DOAJ |
| description | Carbon monoxide (CO) has been proposed as a chemical light signal and neural system modulator via heme oxygenases -1 and -2 (HO-1 and HO-2). Many papers have proven the CO-HO circuit to be important for such physiological pathways as the molecular biological clock and the GnRH axis, but also in such pathological occurrences as ischemic injuries, or inflammation as a regenerative and neuroprotective factor. In this in vivo experiment, we used three groups of pigs: control—housed in natural conditions without any procedures; without CO—adapted and kept in constant darkness, infused with blank plasma; and with CO—adapted and kept in constant darkness infused with CO-enriched plasma. After the experiments, each animal was slaughtered and its eyes were collected for further analysis. Quantitative PCR and Western blot analysis were performed to show statistical differences in the expressions between the experimental groups. Our data revealed that exogenous CO is regulator of mRNA transcription for <i>HO-1</i> and <i>HO-2</i> and <i>PCNA</i>. Moreover, the mRNA abundance of analyzed factors in the experimental group after CO elevation revealed a restored gene-expression level similar to the control group, which we had observed in the group’s restored protein level after CO elevation. In conclusion, exogenous CO regulates <i>HO’s</i> and <i>PCNA</i> gene expression on transcriptional and translational levels in a similar way as a light cue. |
| first_indexed | 2024-03-09T22:32:51Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:32:51Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-66d7eefc700b4edb9882256b1b144ba22023-11-23T18:54:21ZengMDPI AGBiomedicines2227-90592022-02-0110235810.3390/biomedicines10020358Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) ExpressionSławomir Nowak0Przemysław Gilun1Katarzyna Kozioł2Maria Romerowicz-Misielak3Magdalena Koziorowska-Gilun4Barbara Wąsowska5Department of Biotechnology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszów, PolandDepartment of Local Physiological Regulations, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Biotechnology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszów, PolandDepartment of Biotechnology, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszów, PolandDepartment of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, PolandDepartment of Local Physiological Regulations, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10 Str., 10-748 Olsztyn, PolandCarbon monoxide (CO) has been proposed as a chemical light signal and neural system modulator via heme oxygenases -1 and -2 (HO-1 and HO-2). Many papers have proven the CO-HO circuit to be important for such physiological pathways as the molecular biological clock and the GnRH axis, but also in such pathological occurrences as ischemic injuries, or inflammation as a regenerative and neuroprotective factor. In this in vivo experiment, we used three groups of pigs: control—housed in natural conditions without any procedures; without CO—adapted and kept in constant darkness, infused with blank plasma; and with CO—adapted and kept in constant darkness infused with CO-enriched plasma. After the experiments, each animal was slaughtered and its eyes were collected for further analysis. Quantitative PCR and Western blot analysis were performed to show statistical differences in the expressions between the experimental groups. Our data revealed that exogenous CO is regulator of mRNA transcription for <i>HO-1</i> and <i>HO-2</i> and <i>PCNA</i>. Moreover, the mRNA abundance of analyzed factors in the experimental group after CO elevation revealed a restored gene-expression level similar to the control group, which we had observed in the group’s restored protein level after CO elevation. In conclusion, exogenous CO regulates <i>HO’s</i> and <i>PCNA</i> gene expression on transcriptional and translational levels in a similar way as a light cue.https://www.mdpi.com/2227-9059/10/2/358heme oxygenase -1 and -2carbon monoxideretinalight signal |
| spellingShingle | Sławomir Nowak Przemysław Gilun Katarzyna Kozioł Maria Romerowicz-Misielak Magdalena Koziorowska-Gilun Barbara Wąsowska Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression Biomedicines heme oxygenase -1 and -2 carbon monoxide retina light signal |
| title | Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression |
| title_full | Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression |
| title_fullStr | Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression |
| title_full_unstemmed | Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression |
| title_short | Carbon Monoxide (CO) as a Retinal Regulator of Heme Oxygenases -1, and -2 (HO’s) Expression |
| title_sort | carbon monoxide co as a retinal regulator of heme oxygenases 1 and 2 ho s expression |
| topic | heme oxygenase -1 and -2 carbon monoxide retina light signal |
| url | https://www.mdpi.com/2227-9059/10/2/358 |
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