Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering
Amniotic membrane (AM) is an effective and widely used dressing in ocular injuries to reconstruct the cornea. Due to its low mechanical strength, high biodegradation rate, and difficult handling, its usage in medical interventions remains challenging. In this study, decellularized AM was covered wit...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127523010304 |
| _version_ | 1797347815911849984 |
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| author | Zahra Esmaeili Zeinab Nokhbedehghan Sanaz Alizadeh Jila majidi Hadi Chahsetareh Seyed-Hashem Daryabari Maryam Nazm-Bojnourdi Majid Kadkhodaie Maryam Ghaffari Ali Hashemi Hatef Ghasemi Hamidabadi Ahmad Ahmadzadeh Amiri Hajar Nasiri Alireza Dolatshahi-Pirouz Mazaher Gholipourmalekabadi |
| author_facet | Zahra Esmaeili Zeinab Nokhbedehghan Sanaz Alizadeh Jila majidi Hadi Chahsetareh Seyed-Hashem Daryabari Maryam Nazm-Bojnourdi Majid Kadkhodaie Maryam Ghaffari Ali Hashemi Hatef Ghasemi Hamidabadi Ahmad Ahmadzadeh Amiri Hajar Nasiri Alireza Dolatshahi-Pirouz Mazaher Gholipourmalekabadi |
| author_sort | Zahra Esmaeili |
| collection | DOAJ |
| description | Amniotic membrane (AM) is an effective and widely used dressing in ocular injuries to reconstruct the cornea. Due to its low mechanical strength, high biodegradation rate, and difficult handling, its usage in medical interventions remains challenging. In this study, decellularized AM was covered with an ultrathin layer of Polydimethylsiloxane (PDMS) through a spinning method, which in turn resulted in an ultrathin (less than 80 µm in thickness) bilayer corneal wound dressing membrane with improved mechanical behavior and transparency. The biomechanical, biological, and antibacterial properties of the bilayer membranes were measured both in vitro and in vivo. The optimized microsized membrane was applied on a corneal defect wound created in a rabbit model to evaluate the corneal healing. The results demonstrated a significant decrease in degradation rate, improved mechanical properties, and AM/PDMS transparency compared with AM. The corneal transparency improved until 21 days post-surgery in AM/PDMS group. Histological evaluations revealed that AM/PDMS had better epithelial delaminated cell morphology. The results of the RT-PCR showed a significant increase in MMP9, a significant decrease in Col1A1, TGF-β1, TNF-α and IL-6 in both AM and AM/PDMS compared with control wounds. This study suggessts AM/PDMS membrane as an excellent corneal wound dressing. |
| first_indexed | 2024-03-08T11:54:25Z |
| format | Article |
| id | doaj.art-66dd8647280b47159332b38e8fefbd23 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-08T11:54:25Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-66dd8647280b47159332b38e8fefbd232024-01-24T05:16:46ZengElsevierMaterials & Design0264-12752024-01-01237112614Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineeringZahra Esmaeili0Zeinab Nokhbedehghan1Sanaz Alizadeh2Jila majidi3Hadi Chahsetareh4Seyed-Hashem Daryabari5Maryam Nazm-Bojnourdi6Majid Kadkhodaie7Maryam Ghaffari8Ali Hashemi9Hatef Ghasemi Hamidabadi10Ahmad Ahmadzadeh Amiri11Hajar Nasiri12Alireza Dolatshahi-Pirouz13Mazaher Gholipourmalekabadi14Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, IranVision Health Research Center, Semnan University of Medical Sciences, Semnan, IranMazandaran University of Medical Sciences, Department of Anatomical Science, Mazandaran, Sari, IranDepartment of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranBiomaterials Group, Faculty of Biomedical Engineering (Centre of Excellence), Amirkabir University of Technology, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Corresponding authors.Department of Ophthalmology, Clinical Research Development Unit of Bu-Ali Sina Hospital, Faculty of Medicine, Mazandaran University of Medical Sciences, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Health Technology, Technical University of Denmark, 2800 Kgs. Lyngby, DenmarkDepartment of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; NanoBiotechnology & Regenerative Medicine Innovation Group, Noavarn Salamat ZHINO (PHC), Tehran, Iran; Corresponding authors.Amniotic membrane (AM) is an effective and widely used dressing in ocular injuries to reconstruct the cornea. Due to its low mechanical strength, high biodegradation rate, and difficult handling, its usage in medical interventions remains challenging. In this study, decellularized AM was covered with an ultrathin layer of Polydimethylsiloxane (PDMS) through a spinning method, which in turn resulted in an ultrathin (less than 80 µm in thickness) bilayer corneal wound dressing membrane with improved mechanical behavior and transparency. The biomechanical, biological, and antibacterial properties of the bilayer membranes were measured both in vitro and in vivo. The optimized microsized membrane was applied on a corneal defect wound created in a rabbit model to evaluate the corneal healing. The results demonstrated a significant decrease in degradation rate, improved mechanical properties, and AM/PDMS transparency compared with AM. The corneal transparency improved until 21 days post-surgery in AM/PDMS group. Histological evaluations revealed that AM/PDMS had better epithelial delaminated cell morphology. The results of the RT-PCR showed a significant increase in MMP9, a significant decrease in Col1A1, TGF-β1, TNF-α and IL-6 in both AM and AM/PDMS compared with control wounds. This study suggessts AM/PDMS membrane as an excellent corneal wound dressing.http://www.sciencedirect.com/science/article/pii/S0264127523010304Polydimethylsiloxane (PDMS)Corneal tissue engineeringAmniotic membraneSilicone dressingBilayer |
| spellingShingle | Zahra Esmaeili Zeinab Nokhbedehghan Sanaz Alizadeh Jila majidi Hadi Chahsetareh Seyed-Hashem Daryabari Maryam Nazm-Bojnourdi Majid Kadkhodaie Maryam Ghaffari Ali Hashemi Hatef Ghasemi Hamidabadi Ahmad Ahmadzadeh Amiri Hajar Nasiri Alireza Dolatshahi-Pirouz Mazaher Gholipourmalekabadi Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering Materials & Design Polydimethylsiloxane (PDMS) Corneal tissue engineering Amniotic membrane Silicone dressing Bilayer |
| title | Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering |
| title_full | Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering |
| title_fullStr | Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering |
| title_full_unstemmed | Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering |
| title_short | Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering |
| title_sort | biomimetic amniotic silicone based bilayer membrane for corneal tissue engineering |
| topic | Polydimethylsiloxane (PDMS) Corneal tissue engineering Amniotic membrane Silicone dressing Bilayer |
| url | http://www.sciencedirect.com/science/article/pii/S0264127523010304 |
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