Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran
Background & Objective: CD44 is a cellular protein that has been extensively studied for carcinogenicity over the past decade, so the aim of this study is to investigate the expression of CD44 gene in people with lung cancer. Materials & Methods: 30 tissue samples were taken from the lung tu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Fasa University of Medical Sciences
2020-11-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-2352-en.pdf |
| _version_ | 1797679559259193344 |
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| author | Zahra Ahmadzadeh Chaleshtari noosha Zia-jahromi |
| author_facet | Zahra Ahmadzadeh Chaleshtari noosha Zia-jahromi |
| author_sort | Zahra Ahmadzadeh Chaleshtari |
| collection | DOAJ |
| description | Background & Objective: CD44 is a cellular protein that has been extensively studied for carcinogenicity over the past decade, so the aim of this study is to investigate the expression of CD44 gene in people with lung cancer.
Materials & Methods: 30 tissue samples were taken from the lung tumor and 30 samples were taken from healthy lung tissues. RNA samples were extracted and used for cDNA synthesis. The Real Time RT-PCR test with the GAPDH internal control gene was used to assess CD44 gene expression.
Results: CD44 gene expression in tumor tissues, men, age group over 60 years, smokers and adenoma tumor, respectively 1.2, 1.4, 1.1, 1.9 and 1.1 times higher than healthy samples, women, age group Under 60 years of age, non-smokers had SCC tumors.
Conclusion: Inflammation caused by lung cancer increased the production of CD44 inflammatory factor and thus increased its expression. Evaluating the expression of CD44 gene can be used as a marker to predict lung cancer. |
| first_indexed | 2024-03-11T23:16:33Z |
| format | Article |
| id | doaj.art-66ddb7f4aa8045de9f9052574e48df96 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:16:33Z |
| publishDate | 2020-11-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-66ddb7f4aa8045de9f9052574e48df962023-09-20T21:28:07ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232020-11-0110430743084Investigation of CD44 Gene Expression in People with Lung Cancer in TehranZahra Ahmadzadeh Chaleshtari0noosha Zia-jahromi1 Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Background & Objective: CD44 is a cellular protein that has been extensively studied for carcinogenicity over the past decade, so the aim of this study is to investigate the expression of CD44 gene in people with lung cancer. Materials & Methods: 30 tissue samples were taken from the lung tumor and 30 samples were taken from healthy lung tissues. RNA samples were extracted and used for cDNA synthesis. The Real Time RT-PCR test with the GAPDH internal control gene was used to assess CD44 gene expression. Results: CD44 gene expression in tumor tissues, men, age group over 60 years, smokers and adenoma tumor, respectively 1.2, 1.4, 1.1, 1.9 and 1.1 times higher than healthy samples, women, age group Under 60 years of age, non-smokers had SCC tumors. Conclusion: Inflammation caused by lung cancer increased the production of CD44 inflammatory factor and thus increased its expression. Evaluating the expression of CD44 gene can be used as a marker to predict lung cancer.http://jabs.fums.ac.ir/article-1-2352-en.pdflung cancercd44gene expressionreal time rt-pcr |
| spellingShingle | Zahra Ahmadzadeh Chaleshtari noosha Zia-jahromi Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran Journal of Advanced Biomedical Sciences lung cancer cd44 gene expression real time rt-pcr |
| title | Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran |
| title_full | Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran |
| title_fullStr | Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran |
| title_full_unstemmed | Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran |
| title_short | Investigation of CD44 Gene Expression in People with Lung Cancer in Tehran |
| title_sort | investigation of cd44 gene expression in people with lung cancer in tehran |
| topic | lung cancer cd44 gene expression real time rt-pcr |
| url | http://jabs.fums.ac.ir/article-1-2352-en.pdf |
| work_keys_str_mv | AT zahraahmadzadehchaleshtari investigationofcd44geneexpressioninpeoplewithlungcancerintehran AT nooshaziajahromi investigationofcd44geneexpressioninpeoplewithlungcancerintehran |