Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity
Selenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), ri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2023-0036 |
| _version_ | 1797349301513355264 |
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| author | Galić Emerik Radić Kristina Golub Nikolina Mlinar Jakov Čepo Dubravka Vitali Vinković Tomislav |
| author_facet | Galić Emerik Radić Kristina Golub Nikolina Mlinar Jakov Čepo Dubravka Vitali Vinković Tomislav |
| author_sort | Galić Emerik |
| collection | DOAJ |
| description | Selenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyvinylpyrrolidone stabilized and functionalized using OPE (f PVP SeNPs) and polysorbate stabilized and functionalized using OPE (f PS SeNPs) were investigated. SeNPs showed lower toxicity on human hepatocellular carcinoma (HepG2) and human colorectal adenocarcinoma (Caco2) cells compared to selenite. Functionalization with polyphenols significantly improved their direct antiradical (f PVP SeNPs: 24.4 ± 1.84 and f PS SeNPs: 30.9 ± 2.47 mg TE/mmol Se) and reducing properties (f PVP SeNPs: 50 ± 3.16 and f PS SeNPs: 53.6 ± 3.22 mg GAE/mmol) compared to non-functionalized SeNPs. The significant impact of tested SeNPs on intracellular antioxidative mechanisms has been observed and it was dependent on both cell type and physico-chemical properties of SeNPs, indicating the complexity of involved mechanisms. |
| first_indexed | 2024-03-08T12:28:20Z |
| format | Article |
| id | doaj.art-66dea90d1fa54e9286ecb02ffa5de1e4 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-03-08T12:28:20Z |
| publishDate | 2023-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-66dea90d1fa54e9286ecb02ffa5de1e42024-01-22T07:05:24ZengSciendoActa Pharmaceutica1846-95582023-12-0173461763110.2478/acph-2023-0036Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activityGalić Emerik0Radić Kristina1Golub Nikolina2Mlinar Jakov3Čepo Dubravka Vitali4Vinković Tomislav51Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000OsijekCroatia2University of ZagrebFaculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of ZagrebFaculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of ZagrebFaculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of ZagrebFaculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia1Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000OsijekCroatiaSelenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyvinylpyrrolidone stabilized and functionalized using OPE (f PVP SeNPs) and polysorbate stabilized and functionalized using OPE (f PS SeNPs) were investigated. SeNPs showed lower toxicity on human hepatocellular carcinoma (HepG2) and human colorectal adenocarcinoma (Caco2) cells compared to selenite. Functionalization with polyphenols significantly improved their direct antiradical (f PVP SeNPs: 24.4 ± 1.84 and f PS SeNPs: 30.9 ± 2.47 mg TE/mmol Se) and reducing properties (f PVP SeNPs: 50 ± 3.16 and f PS SeNPs: 53.6 ± 3.22 mg GAE/mmol) compared to non-functionalized SeNPs. The significant impact of tested SeNPs on intracellular antioxidative mechanisms has been observed and it was dependent on both cell type and physico-chemical properties of SeNPs, indicating the complexity of involved mechanisms.https://doi.org/10.2478/acph-2023-0036selenium nanoparticlespolyphenolscytotoxicityantioxidative activityreactive oxygen speciesreduced glutathione |
| spellingShingle | Galić Emerik Radić Kristina Golub Nikolina Mlinar Jakov Čepo Dubravka Vitali Vinković Tomislav Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity Acta Pharmaceutica selenium nanoparticles polyphenols cytotoxicity antioxidative activity reactive oxygen species reduced glutathione |
| title | Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity |
| title_full | Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity |
| title_fullStr | Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity |
| title_full_unstemmed | Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity |
| title_short | Functionalization of selenium nanoparticles with olive polyphenols – impact on toxicity and antioxidative activity |
| title_sort | functionalization of selenium nanoparticles with olive polyphenols impact on toxicity and antioxidative activity |
| topic | selenium nanoparticles polyphenols cytotoxicity antioxidative activity reactive oxygen species reduced glutathione |
| url | https://doi.org/10.2478/acph-2023-0036 |
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