Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells
Objective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into contro...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-12-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020120173 |
| _version_ | 1811179848820850688 |
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| author | Yuemei DU Yunfan LIU Fei QIN Liping GAO |
| author_facet | Yuemei DU Yunfan LIU Fei QIN Liping GAO |
| author_sort | Yuemei DU |
| collection | DOAJ |
| description | Objective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into control group, CDDP group, fish oil group, fish oil+CDDP group; the cells were pretreated with different concentrations of fish oil, and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time. Cell viability was detected by CCK-8 method, and the kits were used to measure malondialdehyde (MDA) content, direduced glutathione (GSH) content, and superoxide dismutase (SOD) activity in HEK293 cells. Results: Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP (P<0.05). Fish oil at a concentration of 4 mg/L had the best protective effect, and fish oil at this concentration could significantly inhibit CDDP (20 mg/L) decreased GSH content and increased MDA content (P<0.05). When the fish oil concentration was ≥32 mg/L, the inhibitory effect of CDDP on A549 cells was significantly enhanced (P<0.05). Conclusion: Within a certain concentration range, fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells. Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells, and high-concentration fish oil enhances the anti-cancer effect of CDDP. |
| first_indexed | 2024-04-11T06:40:26Z |
| format | Article |
| id | doaj.art-66df24b006a849cbb04049a9204b3357 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:40:26Z |
| publishDate | 2022-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-66df24b006a849cbb04049a9204b33572022-12-22T04:39:33ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-12-01432334735210.13386/j.issn1002-0306.20201201732020120173-23Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 CellsYuemei DU0Yunfan LIU1Fei QIN2Liping GAO3College of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100023, ChinaObjective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into control group, CDDP group, fish oil group, fish oil+CDDP group; the cells were pretreated with different concentrations of fish oil, and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time. Cell viability was detected by CCK-8 method, and the kits were used to measure malondialdehyde (MDA) content, direduced glutathione (GSH) content, and superoxide dismutase (SOD) activity in HEK293 cells. Results: Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP (P<0.05). Fish oil at a concentration of 4 mg/L had the best protective effect, and fish oil at this concentration could significantly inhibit CDDP (20 mg/L) decreased GSH content and increased MDA content (P<0.05). When the fish oil concentration was ≥32 mg/L, the inhibitory effect of CDDP on A549 cells was significantly enhanced (P<0.05). Conclusion: Within a certain concentration range, fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells. Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells, and high-concentration fish oil enhances the anti-cancer effect of CDDP.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020120173fish oilcisplatinnephrotoxicityanti-oxidantantitumor |
| spellingShingle | Yuemei DU Yunfan LIU Fei QIN Liping GAO Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells Shipin gongye ke-ji fish oil cisplatin nephrotoxicity anti-oxidant antitumor |
| title | Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells |
| title_full | Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells |
| title_fullStr | Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells |
| title_full_unstemmed | Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells |
| title_short | Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells |
| title_sort | effects of fish oil combined with cisplatin on hek293 cells and a549 cells |
| topic | fish oil cisplatin nephrotoxicity anti-oxidant antitumor |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020120173 |
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