Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study
This study investigates the effects of zoledronic acid (ZA) and compressive force on osteoblast functions, to elucidate the pathogenesis of medication-related osteonecrosis of the jaw (MRONJ). MC3T3-E1 cells were exposed to ZA (1, 10 and 100 µM) to evaluate the effects of ZA on cell proliferation. F...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/23/11273 |
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| author | Kazuyuki Yusa Shigeo Ishikawa Tomoharu Hemmi Hiroshi Takano Masayuki Fukuda Mitsuyoshi Iino |
| author_facet | Kazuyuki Yusa Shigeo Ishikawa Tomoharu Hemmi Hiroshi Takano Masayuki Fukuda Mitsuyoshi Iino |
| author_sort | Kazuyuki Yusa |
| collection | DOAJ |
| description | This study investigates the effects of zoledronic acid (ZA) and compressive force on osteoblast functions, to elucidate the pathogenesis of medication-related osteonecrosis of the jaw (MRONJ). MC3T3-E1 cells were exposed to ZA (1, 10 and 100 µM) to evaluate the effects of ZA on cell proliferation. Furthermore, to investigate the influence of ZA with or without compressive force on osteoblast differentiation, real-time polymerase chain reaction and Alizarin Red S staining were performed. ZA concentrations > 10 μM were highly cytotoxic to MC3T3-E1 cells. Combining 1-μM ZA with compressive force influenced expression levels of osteoblast-related genes and matrix mineralization. The inhibitory effects of ZA on cell proliferation and the combination of ZA and compressive force on osteoblast differentiation may contribute to the pathogenesis of MRONJ. |
| first_indexed | 2024-03-10T04:57:13Z |
| format | Article |
| id | doaj.art-66ec352e5dca47b1971b21f90fd0e78a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:57:13Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-66ec352e5dca47b1971b21f90fd0e78a2023-11-23T02:05:27ZengMDPI AGApplied Sciences2076-34172021-11-0111231127310.3390/app112311273Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro StudyKazuyuki Yusa0Shigeo Ishikawa1Tomoharu Hemmi2Hiroshi Takano3Masayuki Fukuda4Mitsuyoshi Iino5Department of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Yamagata University, Yamagata 990-9585, JapanDivision of Dentistry and Oral Surgery, Akita University School of Medicine, Akita 010-8543, JapanDivision of Dentistry and Oral Surgery, Akita University School of Medicine, Akita 010-8543, JapanDepartment of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Yamagata University, Yamagata 990-9585, JapanThis study investigates the effects of zoledronic acid (ZA) and compressive force on osteoblast functions, to elucidate the pathogenesis of medication-related osteonecrosis of the jaw (MRONJ). MC3T3-E1 cells were exposed to ZA (1, 10 and 100 µM) to evaluate the effects of ZA on cell proliferation. Furthermore, to investigate the influence of ZA with or without compressive force on osteoblast differentiation, real-time polymerase chain reaction and Alizarin Red S staining were performed. ZA concentrations > 10 μM were highly cytotoxic to MC3T3-E1 cells. Combining 1-μM ZA with compressive force influenced expression levels of osteoblast-related genes and matrix mineralization. The inhibitory effects of ZA on cell proliferation and the combination of ZA and compressive force on osteoblast differentiation may contribute to the pathogenesis of MRONJ.https://www.mdpi.com/2076-3417/11/23/11273zoledronic acidmedication-related osteonecrosis of the jaw (MRONJ)compressive force |
| spellingShingle | Kazuyuki Yusa Shigeo Ishikawa Tomoharu Hemmi Hiroshi Takano Masayuki Fukuda Mitsuyoshi Iino Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study Applied Sciences zoledronic acid medication-related osteonecrosis of the jaw (MRONJ) compressive force |
| title | Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study |
| title_full | Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study |
| title_fullStr | Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study |
| title_full_unstemmed | Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study |
| title_short | Influences of Compressive Force and Zoledronic Acid on Osteoblast Proliferation and Differentiation: An In Vitro Study |
| title_sort | influences of compressive force and zoledronic acid on osteoblast proliferation and differentiation an in vitro study |
| topic | zoledronic acid medication-related osteonecrosis of the jaw (MRONJ) compressive force |
| url | https://www.mdpi.com/2076-3417/11/23/11273 |
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