Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions
Abstract The zebrafish is widely used as a model in biological studies. In particular, the heart rate and cortisol levels of zebrafish are commonly measured to elucidate the pharmacological effects of chemical substances. Meanwhile, although ventilation is also an important physiological index refle...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22681-4 |
| _version_ | 1797985968643375104 |
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| author | Yuki Harada Zu Soh Shin Wakitani Masayuki Yoshida Toshio Tsuji |
| author_facet | Yuki Harada Zu Soh Shin Wakitani Masayuki Yoshida Toshio Tsuji |
| author_sort | Yuki Harada |
| collection | DOAJ |
| description | Abstract The zebrafish is widely used as a model in biological studies. In particular, the heart rate and cortisol levels of zebrafish are commonly measured to elucidate the pharmacological effects of chemical substances. Meanwhile, although ventilation is also an important physiological index reflecting emotion-like states, few studies have evaluated the effects of chemicals on ventilation in adult zebrafish. In this study, we assessed whether it is possible to evaluate the pharmacological effects elicited by caffeine in adult zebrafish under free-swimming conditions. We measured the ventilation in adult zebrafish exposed to multiple concentrations of caffeine under restraint and free-swimming conditions and evaluated the pharmacological effects of caffeine using linear mixed model analysis. In addition, results of electrocardiogram analysis and swimming speeds were compared with those in previous reports to ensure that an appropriate dose of caffeine was administered. Under restraint conditions, caffeine significantly decreased heart rate and increased ventilation in a concentration-dependent manner. Under free-swimming conditions, the ventilation rate significantly increased with increasing caffeine concentration. These results indicate that the pharmacological effects elicited by chemicals on ventilation can be evaluated in free-swimming zebrafish. |
| first_indexed | 2024-04-11T07:26:27Z |
| format | Article |
| id | doaj.art-66ec36fc25b14eb3b78bf8b61cabee51 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:26:27Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-66ec36fc25b14eb3b78bf8b61cabee512022-12-22T04:37:04ZengNature PortfolioScientific Reports2045-23222022-10-0112111110.1038/s41598-022-22681-4Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditionsYuki Harada0Zu Soh1Shin Wakitani2Masayuki Yoshida3Toshio Tsuji4Graduate School of Advanced Science and Engineering, Hiroshima UniversityGraduate School of Advanced Science and Engineering, Hiroshima UniversityGraduate School of Advanced Science and Engineering, Hiroshima UniversityGraduate School of Integrated Sciences for Life, Hiroshima UniversityGraduate School of Advanced Science and Engineering, Hiroshima UniversityAbstract The zebrafish is widely used as a model in biological studies. In particular, the heart rate and cortisol levels of zebrafish are commonly measured to elucidate the pharmacological effects of chemical substances. Meanwhile, although ventilation is also an important physiological index reflecting emotion-like states, few studies have evaluated the effects of chemicals on ventilation in adult zebrafish. In this study, we assessed whether it is possible to evaluate the pharmacological effects elicited by caffeine in adult zebrafish under free-swimming conditions. We measured the ventilation in adult zebrafish exposed to multiple concentrations of caffeine under restraint and free-swimming conditions and evaluated the pharmacological effects of caffeine using linear mixed model analysis. In addition, results of electrocardiogram analysis and swimming speeds were compared with those in previous reports to ensure that an appropriate dose of caffeine was administered. Under restraint conditions, caffeine significantly decreased heart rate and increased ventilation in a concentration-dependent manner. Under free-swimming conditions, the ventilation rate significantly increased with increasing caffeine concentration. These results indicate that the pharmacological effects elicited by chemicals on ventilation can be evaluated in free-swimming zebrafish.https://doi.org/10.1038/s41598-022-22681-4 |
| spellingShingle | Yuki Harada Zu Soh Shin Wakitani Masayuki Yoshida Toshio Tsuji Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions Scientific Reports |
| title | Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions |
| title_full | Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions |
| title_fullStr | Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions |
| title_full_unstemmed | Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions |
| title_short | Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions |
| title_sort | pharmacological effects of caffeine on ventilation in adult zebrafish under free swimming conditions |
| url | https://doi.org/10.1038/s41598-022-22681-4 |
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