Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing
Adipose browning has demonstrated therapeutic potentials in several diseases. Here, by conducting transcriptomic profiling at the single-cell and single-nucleus resolution, we reconstituted the cellular atlas in mouse inguinal subcutaneous white adipose tissue (iWAT) at thermoneutrality or chronic c...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2023-01-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0182 |
| _version_ | 1797225419466866688 |
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| author | Qing Liu Qiaoyun Long Jiayu Zhao Wenjie Wu Zexin Lin Wei Sun Ping Gu Tuo Deng Kerry Martin Loomes Donghai Wu Alice P. S. Kong Jingying Zhou Alfred S. Cheng Hannah Xiaoyan Hui |
| author_facet | Qing Liu Qiaoyun Long Jiayu Zhao Wenjie Wu Zexin Lin Wei Sun Ping Gu Tuo Deng Kerry Martin Loomes Donghai Wu Alice P. S. Kong Jingying Zhou Alfred S. Cheng Hannah Xiaoyan Hui |
| author_sort | Qing Liu |
| collection | DOAJ |
| description | Adipose browning has demonstrated therapeutic potentials in several diseases. Here, by conducting transcriptomic profiling at the single-cell and single-nucleus resolution, we reconstituted the cellular atlas in mouse inguinal subcutaneous white adipose tissue (iWAT) at thermoneutrality or chronic cold condition. All major nonimmune cells within the iWAT, including adipose stem and progenitor cells (ASPCs), mature adipocytes, endothelial cells, Schwann cells, and smooth muscle cells, were recovered, allowing us to uncover an overall and detailed blueprint for transcriptomes and intercellular cross-talks and the dynamics during white adipose tissue brown remodeling. Our findings also unravel the existence of subpopulations in mature adipocytes, ASPCs, and endothelial cells, as well as new insights on their interconversion and reprogramming in response to cold. The adipocyte subpopulation competent of major histocompatibility complex class II (MHCII) antigen presentation is potentiated. Furthermore, a subcluster of ASPC with CD74 expression was identified as the precursor of this MHCII+ adipocyte. Beige adipocytes are transdifferented from preexisting lipid generating adipocytes, which exhibit developmental trajectory from de novo differentiation of amphiregulin cells (Aregs). Two distinct immune-like endothelial subpopulations are present in iWAT and are responsive to cold. Our data reveal fundamental changes during cold-evoked adipose browning. |
| first_indexed | 2024-03-13T02:32:11Z |
| format | Article |
| id | doaj.art-66ed6c58dd9e4227b2b8c05b539e6a79 |
| institution | Directory Open Access Journal |
| issn | 2639-5274 |
| language | English |
| last_indexed | 2024-04-24T14:08:43Z |
| publishDate | 2023-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj.art-66ed6c58dd9e4227b2b8c05b539e6a792024-04-03T09:37:15ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742023-01-01610.34133/research.0182Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA SequencingQing Liu0Qiaoyun Long1Jiayu Zhao2Wenjie Wu3Zexin Lin4Wei Sun5Ping Gu6Tuo Deng7Kerry Martin Loomes8Donghai Wu9Alice P. S. Kong10Jingying Zhou11Alfred S. Cheng12Hannah Xiaoyan Hui13School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.Department of Pharmacology and Pharmacy, The University of Hong Kong, Hong Kong, China.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.Department of Endocrinology, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, China.National Clinical Research Center for Metabolic Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.School of Biological Sciences and Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand.Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, China.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.Adipose browning has demonstrated therapeutic potentials in several diseases. Here, by conducting transcriptomic profiling at the single-cell and single-nucleus resolution, we reconstituted the cellular atlas in mouse inguinal subcutaneous white adipose tissue (iWAT) at thermoneutrality or chronic cold condition. All major nonimmune cells within the iWAT, including adipose stem and progenitor cells (ASPCs), mature adipocytes, endothelial cells, Schwann cells, and smooth muscle cells, were recovered, allowing us to uncover an overall and detailed blueprint for transcriptomes and intercellular cross-talks and the dynamics during white adipose tissue brown remodeling. Our findings also unravel the existence of subpopulations in mature adipocytes, ASPCs, and endothelial cells, as well as new insights on their interconversion and reprogramming in response to cold. The adipocyte subpopulation competent of major histocompatibility complex class II (MHCII) antigen presentation is potentiated. Furthermore, a subcluster of ASPC with CD74 expression was identified as the precursor of this MHCII+ adipocyte. Beige adipocytes are transdifferented from preexisting lipid generating adipocytes, which exhibit developmental trajectory from de novo differentiation of amphiregulin cells (Aregs). Two distinct immune-like endothelial subpopulations are present in iWAT and are responsive to cold. Our data reveal fundamental changes during cold-evoked adipose browning.https://spj.science.org/doi/10.34133/research.0182 |
| spellingShingle | Qing Liu Qiaoyun Long Jiayu Zhao Wenjie Wu Zexin Lin Wei Sun Ping Gu Tuo Deng Kerry Martin Loomes Donghai Wu Alice P. S. Kong Jingying Zhou Alfred S. Cheng Hannah Xiaoyan Hui Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing Research |
| title | Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing |
| title_full | Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing |
| title_fullStr | Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing |
| title_full_unstemmed | Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing |
| title_short | Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing |
| title_sort | cold induced reprogramming of subcutaneous white adipose tissue assessed by single cell and single nucleus rna sequencing |
| url | https://spj.science.org/doi/10.34133/research.0182 |
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