Rich production media as a platform for CHO cell line development
Abstract Recent cell culture media for mammalian cells can be abundantly formulated with nutrients supporting production, but such media can be limited to use in host cell culture, transfection, cell cloning, and cell growth under the low cell density conditions. In many cases, appropriate platform...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-05-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01025-3 |
| _version_ | 1819148822392078336 |
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| author | Yong Jae Kim Sang Kyul Han Seongtae Yoon Chan Wha Kim |
| author_facet | Yong Jae Kim Sang Kyul Han Seongtae Yoon Chan Wha Kim |
| author_sort | Yong Jae Kim |
| collection | DOAJ |
| description | Abstract Recent cell culture media for mammalian cells can be abundantly formulated with nutrients supporting production, but such media can be limited to use in host cell culture, transfection, cell cloning, and cell growth under the low cell density conditions. In many cases, appropriate platform media are used for cell line development, and then replaced with rich media for production. In this study, we demonstrate rich chemically defined media for Chinese hamster ovary (CHO) cells that are suitable as basal media both for cell line development and for final production of culture process. Set up for transfection, semi-solid media optimization, mini-pool screening, and single cell cloning media development were performed, and final clones were obtained with higher productivity in fed-batch culture mode using rich formulated media comparing with lean formulated media. Developed methods may remove the requirements for cell adaptation to production media after cell line development, and relieve the clonality issues associated with changing the culture media. Furthermore, established methods have advantages over traditional approaches, including saving resources and decreasing the time and the effort required to optimize the production process. |
| first_indexed | 2024-12-22T13:51:49Z |
| format | Article |
| id | doaj.art-66edf1d2bce849558a22f0e890041eef |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-22T13:51:49Z |
| publishDate | 2020-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-66edf1d2bce849558a22f0e890041eef2022-12-21T18:23:38ZengSpringerOpenAMB Express2191-08552020-05-0110111310.1186/s13568-020-01025-3Rich production media as a platform for CHO cell line developmentYong Jae Kim0Sang Kyul Han1Seongtae Yoon2Chan Wha Kim3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea UniversityGC Pharma R &D Center, GC PharmaGC Pharma R &D Center, GC PharmaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea UniversityAbstract Recent cell culture media for mammalian cells can be abundantly formulated with nutrients supporting production, but such media can be limited to use in host cell culture, transfection, cell cloning, and cell growth under the low cell density conditions. In many cases, appropriate platform media are used for cell line development, and then replaced with rich media for production. In this study, we demonstrate rich chemically defined media for Chinese hamster ovary (CHO) cells that are suitable as basal media both for cell line development and for final production of culture process. Set up for transfection, semi-solid media optimization, mini-pool screening, and single cell cloning media development were performed, and final clones were obtained with higher productivity in fed-batch culture mode using rich formulated media comparing with lean formulated media. Developed methods may remove the requirements for cell adaptation to production media after cell line development, and relieve the clonality issues associated with changing the culture media. Furthermore, established methods have advantages over traditional approaches, including saving resources and decreasing the time and the effort required to optimize the production process.http://link.springer.com/article/10.1186/s13568-020-01025-3Cell line developmentChemically defined mediaSingle cell isolationSemi-solid mediaChinese hamster ovary cell |
| spellingShingle | Yong Jae Kim Sang Kyul Han Seongtae Yoon Chan Wha Kim Rich production media as a platform for CHO cell line development AMB Express Cell line development Chemically defined media Single cell isolation Semi-solid media Chinese hamster ovary cell |
| title | Rich production media as a platform for CHO cell line development |
| title_full | Rich production media as a platform for CHO cell line development |
| title_fullStr | Rich production media as a platform for CHO cell line development |
| title_full_unstemmed | Rich production media as a platform for CHO cell line development |
| title_short | Rich production media as a platform for CHO cell line development |
| title_sort | rich production media as a platform for cho cell line development |
| topic | Cell line development Chemically defined media Single cell isolation Semi-solid media Chinese hamster ovary cell |
| url | http://link.springer.com/article/10.1186/s13568-020-01025-3 |
| work_keys_str_mv | AT yongjaekim richproductionmediaasaplatformforchocelllinedevelopment AT sangkyulhan richproductionmediaasaplatformforchocelllinedevelopment AT seongtaeyoon richproductionmediaasaplatformforchocelllinedevelopment AT chanwhakim richproductionmediaasaplatformforchocelllinedevelopment |