Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry
The disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-ci...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Metabolism Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936821000748 |
| _version_ | 1818353397229682688 |
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| author | Dolly L. Crawford Alexis R. Phillips Taylor R. Williams |
| author_facet | Dolly L. Crawford Alexis R. Phillips Taylor R. Williams |
| author_sort | Dolly L. Crawford |
| collection | DOAJ |
| description | The disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-cigarette vapor in a 1L chamber to test the hypothesis that lipid metabolism predominates in vaped mice. Metabolism was quantified via RER using a GA-200 gas analyzer (iWorx, Inc) and LabScribe v.4 (iWorx, Inc.) software. Blood glucose levels were assessed from a subset of the population using an Accu-Check glucometer (Roche Diagnostics, Inc.). Statistical analyses were conducted using R v.4.0.3. Median RER for controls was lower in females. Older females showed a reduction in RER when exposure occurred in the afternoon (p < 0.001), and in males when exposure occurred in the morning (p = 0.007). Glucose concentrations (mg/dL) were higher after e-cigarette inhalation compared with controls, but this difference was not significant (p = 0.464). The reduction in the respiratory exchange ratio supports the hypothesis that e-cigarette inhalation promotes lipid metabolism, and the magnitude of the effect is influenced by gender, age and time of day. |
| first_indexed | 2024-12-13T19:08:53Z |
| format | Article |
| id | doaj.art-66f21b6b7fd14c31adb258340c3dcd43 |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-12-13T19:08:53Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-66f21b6b7fd14c31adb258340c3dcd432022-12-21T23:34:27ZengElsevierMetabolism Open2589-93682021-12-0112100150Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetryDolly L. Crawford0Alexis R. Phillips1Taylor R. Williams2Corresponding author.; Department of Biology and Toxicology, 401 College Avenue, Ashland University, Ashland, OH, 44905, USADepartment of Biology and Toxicology, 401 College Avenue, Ashland University, Ashland, OH, 44905, USADepartment of Biology and Toxicology, 401 College Avenue, Ashland University, Ashland, OH, 44905, USAThe disruption of glucose homeostasis associated with the use of nicotine delivery systems may be due to a shift to lipid metabolism. Indirect calorimetry was used to measure the respiratory exchange ratio (RER) in female (N = 21) and male (N = 21) C57BL/6J mice exposed to room air (control) or e-cigarette vapor in a 1L chamber to test the hypothesis that lipid metabolism predominates in vaped mice. Metabolism was quantified via RER using a GA-200 gas analyzer (iWorx, Inc) and LabScribe v.4 (iWorx, Inc.) software. Blood glucose levels were assessed from a subset of the population using an Accu-Check glucometer (Roche Diagnostics, Inc.). Statistical analyses were conducted using R v.4.0.3. Median RER for controls was lower in females. Older females showed a reduction in RER when exposure occurred in the afternoon (p < 0.001), and in males when exposure occurred in the morning (p = 0.007). Glucose concentrations (mg/dL) were higher after e-cigarette inhalation compared with controls, but this difference was not significant (p = 0.464). The reduction in the respiratory exchange ratio supports the hypothesis that e-cigarette inhalation promotes lipid metabolism, and the magnitude of the effect is influenced by gender, age and time of day.http://www.sciencedirect.com/science/article/pii/S2589936821000748Electronic cigaretteMetabolismRespiratory exchange ratioIndirect calorimetryC57BL/6J miceGender |
| spellingShingle | Dolly L. Crawford Alexis R. Phillips Taylor R. Williams Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry Metabolism Open Electronic cigarette Metabolism Respiratory exchange ratio Indirect calorimetry C57BL/6J mice Gender |
| title | Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry |
| title_full | Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry |
| title_fullStr | Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry |
| title_full_unstemmed | Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry |
| title_short | Evaluation of secondary electronic cigarette inhalation on lipid metabolism in C57BL/6J mice using indirect calorimetry |
| title_sort | evaluation of secondary electronic cigarette inhalation on lipid metabolism in c57bl 6j mice using indirect calorimetry |
| topic | Electronic cigarette Metabolism Respiratory exchange ratio Indirect calorimetry C57BL/6J mice Gender |
| url | http://www.sciencedirect.com/science/article/pii/S2589936821000748 |
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