Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae

Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae

Abstract A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5‐3β‐hydroxysteroid dehydrogenase gene from Digitalis...

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Bibliographic Details
Main Authors: Christoph Rieck, Daniel Geiger, Jennifer Munkert, Katrin Messerschmidt, Jan Petersen, Juliane Strasser, Nadine Meitinger, Wolfgang Kreis
Format: Article
Language:English
Published: Wiley 2019-12-01
Series:MicrobiologyOpen
Subjects:
baker's yeast
biosynthesis
cardenolides
pathway engineering
steroids
Online Access:https://doi.org/10.1002/mbo3.925

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