CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways.
Inflammatory response and articular destruction are common symptoms of osteoarthritis. Cysteine-rich 61 (CCN1 or Cyr61), a secreted protein from the CCN family, is associated with the extracellular matrix involved in many cellular activities like growth and differentiation. Yet the mechanism of CCN1...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4154729?pdf=render |
| _version_ | 1818177728095977472 |
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| author | Cheng-Yu Chen Chen-Ming Su Yuan-Li Huang Chun-Hao Tsai Lih-Jyh Fuh Chih-Hsin Tang |
| author_facet | Cheng-Yu Chen Chen-Ming Su Yuan-Li Huang Chun-Hao Tsai Lih-Jyh Fuh Chih-Hsin Tang |
| author_sort | Cheng-Yu Chen |
| collection | DOAJ |
| description | Inflammatory response and articular destruction are common symptoms of osteoarthritis. Cysteine-rich 61 (CCN1 or Cyr61), a secreted protein from the CCN family, is associated with the extracellular matrix involved in many cellular activities like growth and differentiation. Yet the mechanism of CCN1 interacting with arthritic inflammatory response is unclear. This study finds CCN1 increasing expression of oncostatin m (OSM) in human osteoblastic cells. Pretreatment of αvβ3 monoclonal antibody and inhibitors of focal adhesion kinase (FAK), c-Src, phosphatidylinositol 3-kinase (PI3K), and NF-κB inhibited CCN1-induced OSM expression in osteoblastic cells. Stimulation of cells with CCN1 increased phosphorylation of FAK, c-Src, PI3K, and NF-κB via αvβ3 receptor; CCN1 treatment of osteoblasts increased NF-κB-luciferase activity and p65 binding to NF-κB element on OSM promoter. Results indicate CCN1 heightening OSM expression via αvβ3 receptor, FAK, c-Src, PI3K, and NF-κB signal pathway in osteoblastic cells, suggesting CCN1 as a novel target in arthritis treatment. |
| first_indexed | 2024-12-11T20:36:41Z |
| format | Article |
| id | doaj.art-66f4f11e815748bba2026e508d325230 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T20:36:41Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-66f4f11e815748bba2026e508d3252302022-12-22T00:51:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10663210.1371/journal.pone.0106632CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways.Cheng-Yu ChenChen-Ming SuYuan-Li HuangChun-Hao TsaiLih-Jyh FuhChih-Hsin TangInflammatory response and articular destruction are common symptoms of osteoarthritis. Cysteine-rich 61 (CCN1 or Cyr61), a secreted protein from the CCN family, is associated with the extracellular matrix involved in many cellular activities like growth and differentiation. Yet the mechanism of CCN1 interacting with arthritic inflammatory response is unclear. This study finds CCN1 increasing expression of oncostatin m (OSM) in human osteoblastic cells. Pretreatment of αvβ3 monoclonal antibody and inhibitors of focal adhesion kinase (FAK), c-Src, phosphatidylinositol 3-kinase (PI3K), and NF-κB inhibited CCN1-induced OSM expression in osteoblastic cells. Stimulation of cells with CCN1 increased phosphorylation of FAK, c-Src, PI3K, and NF-κB via αvβ3 receptor; CCN1 treatment of osteoblasts increased NF-κB-luciferase activity and p65 binding to NF-κB element on OSM promoter. Results indicate CCN1 heightening OSM expression via αvβ3 receptor, FAK, c-Src, PI3K, and NF-κB signal pathway in osteoblastic cells, suggesting CCN1 as a novel target in arthritis treatment.http://europepmc.org/articles/PMC4154729?pdf=render |
| spellingShingle | Cheng-Yu Chen Chen-Ming Su Yuan-Li Huang Chun-Hao Tsai Lih-Jyh Fuh Chih-Hsin Tang CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. PLoS ONE |
| title | CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. |
| title_full | CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. |
| title_fullStr | CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. |
| title_full_unstemmed | CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. |
| title_short | CCN1 induces oncostatin M production in osteoblasts via integrin-dependent signal pathways. |
| title_sort | ccn1 induces oncostatin m production in osteoblasts via integrin dependent signal pathways |
| url | http://europepmc.org/articles/PMC4154729?pdf=render |
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