Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay
A competitive colloidal gold lateral flow strip was developed, evaluated, and used as a quick, sensitive, low-cost and convenient tool to identify the authenticity of goat milk on the market. By mixing cow milk with goat milk and testing, the detection range and sensitivity of the strip method were...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-04-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070084 |
| _version_ | 1797983844503126016 |
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| author | Changrui XING Chongjing LIU Zhikang KONG Xiaoxu ZHAO Junqin QIAO Jian YUAN Hongzhen LIAN |
| author_facet | Changrui XING Chongjing LIU Zhikang KONG Xiaoxu ZHAO Junqin QIAO Jian YUAN Hongzhen LIAN |
| author_sort | Changrui XING |
| collection | DOAJ |
| description | A competitive colloidal gold lateral flow strip was developed, evaluated, and used as a quick, sensitive, low-cost and convenient tool to identify the authenticity of goat milk on the market. By mixing cow milk with goat milk and testing, the detection range and sensitivity of the strip method were obtained. The effect of boiling and hydrolysis to strip were evaluated. The detection of α-casein and β-casein from cow and their hydrolysate by strip were also finished. Based on UPLC-Q-Exactive Orbitrap MS and the casein antibody affinity column, the purified peptides was identified by Peaks software. The identified peptides were artificially synthesized and tested by strips. The results showed that the limit of detection for qualitative judgment of goat milk adulteration was, by the naked eye, 1.2 mg/100 g and the cut-off value was 6 mg/100 g. The boiling and hydrolysis process had no effect to detection. At the same time, α-casein and β-casein could be detected, indicating this method could not distinguish and test the enzymatic hydrolysis α-casein and β-casein. Most of peptides obtained by multi-enzymes digestion could not bind to the casein antibody and the strip results indicating the casein antibody could not interact with artificially synthesized peptides too. In total, this strip could determine the intact structure of bovine casein in goat milk matrix, and could be used as an effective tool for authenticity identification. |
| first_indexed | 2024-04-11T06:52:26Z |
| format | Article |
| id | doaj.art-66f5b7c06706428d9f108d2fabd54681 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:52:26Z |
| publishDate | 2022-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-66f5b7c06706428d9f108d2fabd546812022-12-22T04:39:08ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-04-0143828128710.13386/j.issn1002-0306.20210700842021070084-8Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid ImmunoassayChangrui XING0Chongjing LIU1Zhikang KONG2Xiaoxu ZHAO3Junqin QIAO4Jian YUAN5Hongzhen LIAN6College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaBeijing Meizheng Biotechnology Co., Ltd., Beijing 102100, ChinaState Key Laboratory of Analytical Chemistry for Life Science, College of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaState Key Laboratory of Analytical Chemistry for Life Science, College of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210023, ChinaA competitive colloidal gold lateral flow strip was developed, evaluated, and used as a quick, sensitive, low-cost and convenient tool to identify the authenticity of goat milk on the market. By mixing cow milk with goat milk and testing, the detection range and sensitivity of the strip method were obtained. The effect of boiling and hydrolysis to strip were evaluated. The detection of α-casein and β-casein from cow and their hydrolysate by strip were also finished. Based on UPLC-Q-Exactive Orbitrap MS and the casein antibody affinity column, the purified peptides was identified by Peaks software. The identified peptides were artificially synthesized and tested by strips. The results showed that the limit of detection for qualitative judgment of goat milk adulteration was, by the naked eye, 1.2 mg/100 g and the cut-off value was 6 mg/100 g. The boiling and hydrolysis process had no effect to detection. At the same time, α-casein and β-casein could be detected, indicating this method could not distinguish and test the enzymatic hydrolysis α-casein and β-casein. Most of peptides obtained by multi-enzymes digestion could not bind to the casein antibody and the strip results indicating the casein antibody could not interact with artificially synthesized peptides too. In total, this strip could determine the intact structure of bovine casein in goat milk matrix, and could be used as an effective tool for authenticity identification.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070084caseincow milkgoat milkcolloidal gold test stripauthentic identification |
| spellingShingle | Changrui XING Chongjing LIU Zhikang KONG Xiaoxu ZHAO Junqin QIAO Jian YUAN Hongzhen LIAN Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay Shipin gongye ke-ji casein cow milk goat milk colloidal gold test strip authentic identification |
| title | Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay |
| title_full | Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay |
| title_fullStr | Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay |
| title_full_unstemmed | Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay |
| title_short | Establishment of Goat Milk Forgery Detection Method Based on Casein Rapid Immunoassay |
| title_sort | establishment of goat milk forgery detection method based on casein rapid immunoassay |
| topic | casein cow milk goat milk colloidal gold test strip authentic identification |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070084 |
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