Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices
To reduce the polymerization shrinkage of dental composite resin, we used two different ratios of toluene 2,4-diisocyanate (TDI) or 1,6-hexamethylene diisocyanate (HDI) as functional side chains of bisphenol A-glycidyl methacrylate (bis-GMA) to synthesize two series of new dental resin matrices. Thi...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | Journal of the Formosan Medical Association |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664612003634 |
| _version_ | 1818944449219133440 |
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| author | Yih-Dean Jan Bor-Shiunn Lee Chun-Pin Lin Wan-Yu Tseng |
| author_facet | Yih-Dean Jan Bor-Shiunn Lee Chun-Pin Lin Wan-Yu Tseng |
| author_sort | Yih-Dean Jan |
| collection | DOAJ |
| description | To reduce the polymerization shrinkage of dental composite resin, we used two different ratios of toluene 2,4-diisocyanate (TDI) or 1,6-hexamethylene diisocyanate (HDI) as functional side chains of bisphenol A-glycidyl methacrylate (bis-GMA) to synthesize two series of new dental resin matrices. This study evaluated the biocompatibility and cytotoxicity of these two series of new resin matrices.
Methods: Two series of new dental resin matrices with the ratios of TDI or HDI functional side chain to bis-GMA (defined as B group) being 1:4, 1:2, 1:1 and 3:2 (defined as T1/4, T1/2, T1, T3/2, and H1/4, H1/2, H1, H3/2 groups, respectively) were synthesized. Each resin sample was light cured and immersed in the culture medium for 24 hours to make the extract solution. Then, human gingival fibroblasts were cultured in different extract solutions for 72 hours. The cytotoxicities of different resins were evaluated by microtitertetrazolium (MTT) assay, the levels of cell-produced reactive oxygen species (ROS) induced by different extract solutions was measured.
Results: Resins of the T1/4 and B groups revealed significantly higher cytotoxicity than resins of other groups. However, resins of the T1 and T3/2 groups exhibited less cytotoxicity. In general, resins of the TDI-modified groups showed equal or less cytotoxicity and induced equal or lower levels of ROS than the corresponding resins of the HDI-modified and B groups.
Conclusion: Our results showed that the TDI-modified resin matrices containing more functional side chains were less cytotoxic than the corresponding HDI-modified resin matrices. When the ratio of functional side chain to bis-GMA is increased, the stereo hindrance of resin structure is increased, more toxic resin monomers are trapped in the complicated resin structure, and thus the resin matrix reveals less cytotoxicity. The TDI-modified resin matrices exhibit higher stereo hindrance of resin structure and thus show less cytotoxicity than the corresponding HDI-modified resin matrices. |
| first_indexed | 2024-12-20T07:43:24Z |
| format | Article |
| id | doaj.art-66f63a75bb2d4856a7c9ae55443526aa |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-20T07:43:24Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-66f63a75bb2d4856a7c9ae55443526aa2022-12-21T19:48:04ZengElsevierJournal of the Formosan Medical Association0929-66462014-06-01113634935510.1016/j.jfma.2012.07.008Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matricesYih-Dean Jan0Bor-Shiunn Lee1Chun-Pin Lin2Wan-Yu Tseng3Graduate Institute of Clinical Dentistry, National Taiwan University, Taipei, TaiwanGraduate Institute of Oral Biology, National Taiwan University, Taipei, TaiwanGraduate Institute of Clinical Dentistry, National Taiwan University, Taipei, TaiwanSchool of Dentistry, National Taiwan University, Taipei, TaiwanTo reduce the polymerization shrinkage of dental composite resin, we used two different ratios of toluene 2,4-diisocyanate (TDI) or 1,6-hexamethylene diisocyanate (HDI) as functional side chains of bisphenol A-glycidyl methacrylate (bis-GMA) to synthesize two series of new dental resin matrices. This study evaluated the biocompatibility and cytotoxicity of these two series of new resin matrices. Methods: Two series of new dental resin matrices with the ratios of TDI or HDI functional side chain to bis-GMA (defined as B group) being 1:4, 1:2, 1:1 and 3:2 (defined as T1/4, T1/2, T1, T3/2, and H1/4, H1/2, H1, H3/2 groups, respectively) were synthesized. Each resin sample was light cured and immersed in the culture medium for 24 hours to make the extract solution. Then, human gingival fibroblasts were cultured in different extract solutions for 72 hours. The cytotoxicities of different resins were evaluated by microtitertetrazolium (MTT) assay, the levels of cell-produced reactive oxygen species (ROS) induced by different extract solutions was measured. Results: Resins of the T1/4 and B groups revealed significantly higher cytotoxicity than resins of other groups. However, resins of the T1 and T3/2 groups exhibited less cytotoxicity. In general, resins of the TDI-modified groups showed equal or less cytotoxicity and induced equal or lower levels of ROS than the corresponding resins of the HDI-modified and B groups. Conclusion: Our results showed that the TDI-modified resin matrices containing more functional side chains were less cytotoxic than the corresponding HDI-modified resin matrices. When the ratio of functional side chain to bis-GMA is increased, the stereo hindrance of resin structure is increased, more toxic resin monomers are trapped in the complicated resin structure, and thus the resin matrix reveals less cytotoxicity. The TDI-modified resin matrices exhibit higher stereo hindrance of resin structure and thus show less cytotoxicity than the corresponding HDI-modified resin matrices.http://www.sciencedirect.com/science/article/pii/S0929664612003634biocompatibilitycytotoxicityreactive oxygen speciesresin matrix |
| spellingShingle | Yih-Dean Jan Bor-Shiunn Lee Chun-Pin Lin Wan-Yu Tseng Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices Journal of the Formosan Medical Association biocompatibility cytotoxicity reactive oxygen species resin matrix |
| title | Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices |
| title_full | Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices |
| title_fullStr | Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices |
| title_full_unstemmed | Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices |
| title_short | Biocompatibility and cytotoxicity of two novel low-shrinkage dental resin matrices |
| title_sort | biocompatibility and cytotoxicity of two novel low shrinkage dental resin matrices |
| topic | biocompatibility cytotoxicity reactive oxygen species resin matrix |
| url | http://www.sciencedirect.com/science/article/pii/S0929664612003634 |
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