Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources
Abstract Polysaccharide is widely distributed in natural resources, and currently attracts scientists' attention for their various bio‐functions including immunomodulatory activity, hypoglycemic activity, hypolipidemic activity, antitumor activity, promoting effects on gastrointestinal function...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-12-01
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| Series: | eFood |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/efd2.37 |
| _version_ | 1828071149126287360 |
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| author | Xiaojun Huang Chao Ai Haoyingye Yao Chengang Zhao Chunhong Xiang Tao Hong Jianbo Xiao |
| author_facet | Xiaojun Huang Chao Ai Haoyingye Yao Chengang Zhao Chunhong Xiang Tao Hong Jianbo Xiao |
| author_sort | Xiaojun Huang |
| collection | DOAJ |
| description | Abstract Polysaccharide is widely distributed in natural resources, and currently attracts scientists' attention for their various bio‐functions including immunomodulatory activity, hypoglycemic activity, hypolipidemic activity, antitumor activity, promoting effects on gastrointestinal function, and so on. The purity and structure characteristics of different polysaccharides greatly restrict the in‐depth study of their bioactivity mechanism. Therefore, this guideline will display the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources. The extraction methods of acid‐soluble polysaccharides, water‐soluble polysaccharides, and alkaline soluble polysaccharides are included. Common purification methods like membrane filtration, alcohol precipitation, and column chromatographic separation are explained in detail. A comprehensive procedure for polysaccharide structural characterization is given from molecular weight and monosaccharide composition study to Fourier transform infrared and nuclear magnetic resonance analysis. Examples are added where necessary to help you better understand this guideline. |
| first_indexed | 2024-04-11T00:50:05Z |
| format | Article |
| id | doaj.art-66f78269583341189ca9c7308b1a028e |
| institution | Directory Open Access Journal |
| issn | 2666-3066 |
| language | English |
| last_indexed | 2024-04-11T00:50:05Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | eFood |
| spelling | doaj.art-66f78269583341189ca9c7308b1a028e2023-01-05T09:28:04ZengWileyeFood2666-30662022-12-0136n/an/a10.1002/efd2.37Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resourcesXiaojun Huang0Chao Ai1Haoyingye Yao2Chengang Zhao3Chunhong Xiang4Tao Hong5Jianbo Xiao6State Key Laboratory of Food Science and Technology, China‐Canada Joint Lab of Food Science and Technology (Nanchang) Nanchang University Nanchang ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology Guangdong Ocean University Zhanjiang ChinaState Key Laboratory of Food Science and Technology, China‐Canada Joint Lab of Food Science and Technology (Nanchang) Nanchang University Nanchang ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology Guangdong Ocean University Zhanjiang ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology Guangdong Ocean University Zhanjiang ChinaState Key Laboratory of Food Science and Technology, China‐Canada Joint Lab of Food Science and Technology (Nanchang) Nanchang University Nanchang ChinaDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology University of Vigo—Ourense Campus Ourense SpainAbstract Polysaccharide is widely distributed in natural resources, and currently attracts scientists' attention for their various bio‐functions including immunomodulatory activity, hypoglycemic activity, hypolipidemic activity, antitumor activity, promoting effects on gastrointestinal function, and so on. The purity and structure characteristics of different polysaccharides greatly restrict the in‐depth study of their bioactivity mechanism. Therefore, this guideline will display the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources. The extraction methods of acid‐soluble polysaccharides, water‐soluble polysaccharides, and alkaline soluble polysaccharides are included. Common purification methods like membrane filtration, alcohol precipitation, and column chromatographic separation are explained in detail. A comprehensive procedure for polysaccharide structural characterization is given from molecular weight and monosaccharide composition study to Fourier transform infrared and nuclear magnetic resonance analysis. Examples are added where necessary to help you better understand this guideline.https://doi.org/10.1002/efd2.37extractionpolysaccharidepurificationstructural characterization |
| spellingShingle | Xiaojun Huang Chao Ai Haoyingye Yao Chengang Zhao Chunhong Xiang Tao Hong Jianbo Xiao Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources eFood extraction polysaccharide purification structural characterization |
| title | Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources |
| title_full | Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources |
| title_fullStr | Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources |
| title_full_unstemmed | Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources |
| title_short | Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources |
| title_sort | guideline for the extraction isolation purification and structural characterization of polysaccharides from natural resources |
| topic | extraction polysaccharide purification structural characterization |
| url | https://doi.org/10.1002/efd2.37 |
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