Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women
Women with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.01108/full |
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| author | Yaoyao Tu Yaoyao Tu Guangyong Zheng Guolian Ding Guolian Ding Yanting Wu Yanting Wu Ji Xi Yingzhou Ge Yingzhou Ge Hangchao Gu Hangchao Gu Yingyu Wang Yingyu Wang Jianzhong Sheng Jianzhong Sheng Xinmei Liu Xinmei Liu Li Jin Li Jin Hefeng Huang Hefeng Huang |
| author_facet | Yaoyao Tu Yaoyao Tu Guangyong Zheng Guolian Ding Guolian Ding Yanting Wu Yanting Wu Ji Xi Yingzhou Ge Yingzhou Ge Hangchao Gu Hangchao Gu Yingyu Wang Yingyu Wang Jianzhong Sheng Jianzhong Sheng Xinmei Liu Xinmei Liu Li Jin Li Jin Hefeng Huang Hefeng Huang |
| author_sort | Yaoyao Tu |
| collection | DOAJ |
| description | Women with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotterdam Criteria) and 50 healthy reproductive-aged controls in this study. Variable regions 3–4 (V3–4) were sequenced and analyzed. Operational taxonomic unit (OTU) abundance was noted for all samples. Taxa that discriminated between PCOS and healthy women was calculated by linear discriminant analysis effect size (LEFSe). Results from 97 paired vaginal and cervical canal samples collected from 97 women [mean age 30 (±4 years)] were available for analysis. Using the Rotterdam Criteria, 47 women were diagnosed with PCOS (PCOS, n = 47; control, n = 50). There was no significant difference between cervical canal microbiome and vaginal microbiome from the same individual, however, Lactobacillus spp. was less abundant in both vaginal and cervical canal microbiome of PCOS patients. Several non-Lactobacillus taxa including Gardnerella_vaginalis_00703mash, Prevotella_9_other, and Mycoplasma hominis, were more abundant in the LGT microbiota of PCOS patients. There is a difference between the microorganism in the LGT of patients with PCOS and healthy reproductive-aged women. |
| first_indexed | 2024-12-13T09:48:29Z |
| format | Article |
| id | doaj.art-66f7cfdb52f34b35ba5e523f3d789cd3 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-13T09:48:29Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-66f7cfdb52f34b35ba5e523f3d789cd32022-12-21T23:51:59ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.01108563753Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy WomenYaoyao Tu0Yaoyao Tu1Guangyong Zheng2Guolian Ding3Guolian Ding4Yanting Wu5Yanting Wu6Ji Xi7Yingzhou Ge8Yingzhou Ge9Hangchao Gu10Hangchao Gu11Yingyu Wang12Yingyu Wang13Jianzhong Sheng14Jianzhong Sheng15Xinmei Liu16Xinmei Liu17Li Jin18Li Jin19Hefeng Huang20Hefeng Huang21Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaBio-Med Big Data Center, CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaKey Laboratory of Reproductive Genetics, Ministry of Education, Zhejiang University, Hangzhou, ChinaDepartment of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Diseases, Shanghai, ChinaWomen with polycystic ovarian syndrome (PCOS) often have a history of infertility and poor pregnancy outcome. The character of the lower genital tract (LGT) microbiome of these patients is still unknown. We collected both vaginal and cervical canal swabs from 47 PCOS patients (diagnosed by the Rotterdam Criteria) and 50 healthy reproductive-aged controls in this study. Variable regions 3–4 (V3–4) were sequenced and analyzed. Operational taxonomic unit (OTU) abundance was noted for all samples. Taxa that discriminated between PCOS and healthy women was calculated by linear discriminant analysis effect size (LEFSe). Results from 97 paired vaginal and cervical canal samples collected from 97 women [mean age 30 (±4 years)] were available for analysis. Using the Rotterdam Criteria, 47 women were diagnosed with PCOS (PCOS, n = 47; control, n = 50). There was no significant difference between cervical canal microbiome and vaginal microbiome from the same individual, however, Lactobacillus spp. was less abundant in both vaginal and cervical canal microbiome of PCOS patients. Several non-Lactobacillus taxa including Gardnerella_vaginalis_00703mash, Prevotella_9_other, and Mycoplasma hominis, were more abundant in the LGT microbiota of PCOS patients. There is a difference between the microorganism in the LGT of patients with PCOS and healthy reproductive-aged women.https://www.frontiersin.org/article/10.3389/fphys.2020.01108/fullGardnerellaLactobacilluslower genital tract (LGT)microbiomePCOS |
| spellingShingle | Yaoyao Tu Yaoyao Tu Guangyong Zheng Guolian Ding Guolian Ding Yanting Wu Yanting Wu Ji Xi Yingzhou Ge Yingzhou Ge Hangchao Gu Hangchao Gu Yingyu Wang Yingyu Wang Jianzhong Sheng Jianzhong Sheng Xinmei Liu Xinmei Liu Li Jin Li Jin Hefeng Huang Hefeng Huang Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women Frontiers in Physiology Gardnerella Lactobacillus lower genital tract (LGT) microbiome PCOS |
| title | Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women |
| title_full | Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women |
| title_fullStr | Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women |
| title_full_unstemmed | Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women |
| title_short | Comparative Analysis of Lower Genital Tract Microbiome Between PCOS and Healthy Women |
| title_sort | comparative analysis of lower genital tract microbiome between pcos and healthy women |
| topic | Gardnerella Lactobacillus lower genital tract (LGT) microbiome PCOS |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.01108/full |
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