Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy
Abstract Background The endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-05-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-021-00121-y |
| _version_ | 1818641486889091072 |
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| author | Jayarami Reddy Medapati Deepthi Rapaka Veera Raghavulu Bitra Santhosh Kumar Ranajit Girija Sankar Guntuku Annapurna Akula |
| author_facet | Jayarami Reddy Medapati Deepthi Rapaka Veera Raghavulu Bitra Santhosh Kumar Ranajit Girija Sankar Guntuku Annapurna Akula |
| author_sort | Jayarami Reddy Medapati |
| collection | DOAJ |
| description | Abstract Background The endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present work investigates the possible role of CB1 receptors in the pathogenesis of diabetes-induced nephropathy. Streptozotocin (STZ) (55 mg/kg, i.p., once) is administered to uninephrectomised rats for induction of experimental diabetes mellitus. The CB1 agonist (oleamide) and CB1 antagonist (AM6545) treatment were initiated in diabetic rats after 1 week of STZ administration and were given for 24 weeks. Results The progress in diabetic nephropathy is estimated biochemically by measuring serum creatinine (1.28±0.03) (p < 0.005), blood urea nitrogen (67.6± 2.10) (p < 0.001), urinary microprotein (74.62± 3.47) (p < 0.005) and urinary albuminuria (28.31±1.17) (p < 0.0001). Renal inflammation was assessed by estimating serum levels of tumor necrosis factor alpha (75.69±1.51) (p < 0.001) and transforming growth factor beta (8.73±0.31) (p < 0.001). Renal morphological changes were assessed by estimating renal hypertrophy (7.38± 0.26) (p < 0.005) and renal collagen content (10.42± 0.48) (p < 0.001). Conclusions From the above findings, it can be said that diabetes-induced nephropathy may be associated with overexpression of CB1 receptors and blockade of CB1 receptors might be beneficial in ameliorating the diabetes-induced nephropathy. Graphical abstract |
| first_indexed | 2024-12-16T23:27:56Z |
| format | Article |
| id | doaj.art-66f7f9ad6c834785b6bd97ddb1e3ed55 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-16T23:27:56Z |
| publishDate | 2021-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-66f7f9ad6c834785b6bd97ddb1e3ed552022-12-21T22:11:56ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432021-05-0110111210.1186/s43088-021-00121-yRole of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathyJayarami Reddy Medapati0Deepthi Rapaka1Veera Raghavulu Bitra2Santhosh Kumar Ranajit3Girija Sankar Guntuku4Annapurna Akula5A U College of Pharmaceutical Sciences, Andhra UniversityA U College of Pharmaceutical Sciences, Andhra UniversityA U College of Pharmaceutical Sciences, Andhra UniversityPharmacology Division, Vignan Institute of Pharmacuetical TechnologyA U College of Pharmaceutical Sciences, Andhra UniversityA U College of Pharmaceutical Sciences, Andhra UniversityAbstract Background The endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present work investigates the possible role of CB1 receptors in the pathogenesis of diabetes-induced nephropathy. Streptozotocin (STZ) (55 mg/kg, i.p., once) is administered to uninephrectomised rats for induction of experimental diabetes mellitus. The CB1 agonist (oleamide) and CB1 antagonist (AM6545) treatment were initiated in diabetic rats after 1 week of STZ administration and were given for 24 weeks. Results The progress in diabetic nephropathy is estimated biochemically by measuring serum creatinine (1.28±0.03) (p < 0.005), blood urea nitrogen (67.6± 2.10) (p < 0.001), urinary microprotein (74.62± 3.47) (p < 0.005) and urinary albuminuria (28.31±1.17) (p < 0.0001). Renal inflammation was assessed by estimating serum levels of tumor necrosis factor alpha (75.69±1.51) (p < 0.001) and transforming growth factor beta (8.73±0.31) (p < 0.001). Renal morphological changes were assessed by estimating renal hypertrophy (7.38± 0.26) (p < 0.005) and renal collagen content (10.42± 0.48) (p < 0.001). Conclusions From the above findings, it can be said that diabetes-induced nephropathy may be associated with overexpression of CB1 receptors and blockade of CB1 receptors might be beneficial in ameliorating the diabetes-induced nephropathy. Graphical abstracthttps://doi.org/10.1186/s43088-021-00121-yDiabetic nephropathyAlbuminuriaUninephrectomyOleamide |
| spellingShingle | Jayarami Reddy Medapati Deepthi Rapaka Veera Raghavulu Bitra Santhosh Kumar Ranajit Girija Sankar Guntuku Annapurna Akula Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy Beni-Suef University Journal of Basic and Applied Sciences Diabetic nephropathy Albuminuria Uninephrectomy Oleamide |
| title | Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy |
| title_full | Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy |
| title_fullStr | Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy |
| title_full_unstemmed | Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy |
| title_short | Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy |
| title_sort | role of endocannabinoid cb1 receptors in streptozotocin induced uninephrectomised wistar rats in diabetic nephropathy |
| topic | Diabetic nephropathy Albuminuria Uninephrectomy Oleamide |
| url | https://doi.org/10.1186/s43088-021-00121-y |
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