Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India
Fluoroquinolones (FQ) are integral part of multidrug resistant/rifampicin resistant tuberculosis (MDR-TB/RR TB) treatment regimens. FQ resistance in MDR-TB lead to narrower treatment options and poor treatment outcomes. Hence proportion of FQ resistance among MDR-TB and cross-resistance among FQ in...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2021-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/additional-resistance-to-any-fluoroquinolones-among-multidrug-resistant-mycobacterium-tuberculosis-isolates-from-north-coastal-andhra-pradesh-india/ |
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| author | Kancharla Suresh Yapadinna Vimala Nitin Mohan Indugula Jyothi Padmaja |
| author_facet | Kancharla Suresh Yapadinna Vimala Nitin Mohan Indugula Jyothi Padmaja |
| author_sort | Kancharla Suresh |
| collection | DOAJ |
| description | Fluoroquinolones (FQ) are integral part of multidrug resistant/rifampicin resistant tuberculosis (MDR-TB/RR TB) treatment regimens. FQ resistance in MDR-TB lead to narrower treatment options and poor treatment outcomes. Hence proportion of FQ resistance among MDR-TB and cross-resistance among FQ in MDR-TB isolates was studied. This is the first study from North Coastal Andhra Pradesh, India. A cross-sectional study was conducted on 21,474 sputum samples from January-December 2018 & 833 culture isolates (rifampicin resistant detected by GeneXpert MTB / RIF assay & MDR-TB detected by direct and indirect GenoType MTBDRplus Assay) were analysed for FQ resistance by Mycobacterium Growth Indicator Tube (MGIT) for ofloxacin (Ofx) 2.0 µg/mL, levofloxacin (Lfx) 1.5 µg/mL & moxifloxacin (Mfx) 2.0 μg/mL. Among 833 culture isolates, 119 (14.2%) showed resistance to FQ which included 90 (10.8%) Ofx and Lfx resistance isolates but still sensitive to Mfx, 16 (1.92%) with resistance to all FQ tested, 12 Ofx monoresistance & 1 Lfx monoresistance. Mfx monoresistance was absent. Mfx sensitive isolates are 714 (85.7%) and were always sensitive to Ofx and Lfx. Cross-resistance was not complete. MDR-TB/RR TB is 4.1% among all TB patients. Additional resistance to FQ, among MDR-TB patients was seen in 14.2% patients. |
| first_indexed | 2024-12-19T19:45:25Z |
| format | Article |
| id | doaj.art-66f871ed801343aab6c6c2128db70976 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-19T19:45:25Z |
| publishDate | 2021-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-66f871ed801343aab6c6c2128db709762022-12-21T20:08:08ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-03-01151687410.22207/JPAM.15.1.01Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, IndiaKancharla Sureshhttps://orcid.org/0000-0003-2590-6653Yapadinna Vimalahttps://orcid.org/0000-0002-5019-0987Nitin Mohanhttps://orcid.org/0000-0001-8813-3147Indugula Jyothi Padmajahttps://orcid.org/0000-0003-2265-5836Fluoroquinolones (FQ) are integral part of multidrug resistant/rifampicin resistant tuberculosis (MDR-TB/RR TB) treatment regimens. FQ resistance in MDR-TB lead to narrower treatment options and poor treatment outcomes. Hence proportion of FQ resistance among MDR-TB and cross-resistance among FQ in MDR-TB isolates was studied. This is the first study from North Coastal Andhra Pradesh, India. A cross-sectional study was conducted on 21,474 sputum samples from January-December 2018 & 833 culture isolates (rifampicin resistant detected by GeneXpert MTB / RIF assay & MDR-TB detected by direct and indirect GenoType MTBDRplus Assay) were analysed for FQ resistance by Mycobacterium Growth Indicator Tube (MGIT) for ofloxacin (Ofx) 2.0 µg/mL, levofloxacin (Lfx) 1.5 µg/mL & moxifloxacin (Mfx) 2.0 μg/mL. Among 833 culture isolates, 119 (14.2%) showed resistance to FQ which included 90 (10.8%) Ofx and Lfx resistance isolates but still sensitive to Mfx, 16 (1.92%) with resistance to all FQ tested, 12 Ofx monoresistance & 1 Lfx monoresistance. Mfx monoresistance was absent. Mfx sensitive isolates are 714 (85.7%) and were always sensitive to Ofx and Lfx. Cross-resistance was not complete. MDR-TB/RR TB is 4.1% among all TB patients. Additional resistance to FQ, among MDR-TB patients was seen in 14.2% patients.https://microbiologyjournal.org/additional-resistance-to-any-fluoroquinolones-among-multidrug-resistant-mycobacterium-tuberculosis-isolates-from-north-coastal-andhra-pradesh-india/multidrug-resistant tuberculosisfluoroquinolonesmgit 960cross-resistance |
| spellingShingle | Kancharla Suresh Yapadinna Vimala Nitin Mohan Indugula Jyothi Padmaja Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India Journal of Pure and Applied Microbiology multidrug-resistant tuberculosis fluoroquinolones mgit 960 cross-resistance |
| title | Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India |
| title_full | Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India |
| title_fullStr | Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India |
| title_full_unstemmed | Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India |
| title_short | Additional Resistance to any Fluoroquinolones among Multidrug-resistant Mycobacterium tuberculosis Isolates from North Coastal Andhra Pradesh, India |
| title_sort | additional resistance to any fluoroquinolones among multidrug resistant mycobacterium tuberculosis isolates from north coastal andhra pradesh india |
| topic | multidrug-resistant tuberculosis fluoroquinolones mgit 960 cross-resistance |
| url | https://microbiologyjournal.org/additional-resistance-to-any-fluoroquinolones-among-multidrug-resistant-mycobacterium-tuberculosis-isolates-from-north-coastal-andhra-pradesh-india/ |
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