Effect of Daidzein intravitreal injection on optic nerve injury in rats
AIM: To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages. <p>METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control gro...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-08-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://www.ies.net.cn/cn_publish/2014/8/201408003.pdf |
| _version_ | 1819138165141667840 |
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| author | Zhong-Jun Tang Zhen-Ping Huang Wen-Jing Yang Yong-Xiang Zou Ji-Ping Cai |
| author_facet | Zhong-Jun Tang Zhen-Ping Huang Wen-Jing Yang Yong-Xiang Zou Ji-Ping Cai |
| author_sort | Zhong-Jun Tang |
| collection | DOAJ |
| description | AIM: To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages. <p>METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group(10μmol/L, 100μmol/L, 1000μmol/L)and positive control group using rats nerve growth factor(mNGF, 100ng/mL). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups. <p>RESULTS:Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance(<i>P</i><0.05).<p>CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages. |
| first_indexed | 2024-12-22T11:02:26Z |
| format | Article |
| id | doaj.art-66f888d96ac84dbeb67fba8657a76268 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-22T11:02:26Z |
| publishDate | 2014-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-66f888d96ac84dbeb67fba8657a762682022-12-21T18:28:27ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232014-08-011481378138110.3980/j.issn.1672-5123.2014.08.03Effect of Daidzein intravitreal injection on optic nerve injury in ratsZhong-Jun Tang0Zhen-Ping Huang1Wen-Jing Yang2Yong-Xiang Zou3Ji-Ping Cai4Department of Ophthalmology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, ChinaDepartment of Ophthalmology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210000, Jiangsu Province, ChinaTeaching and Research Office of Neurobiology, School of Basic Medical Sciences, the Second Military Medical University, Shanghai 200003, ChinaDepartment of Neurosurgery, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, ChinaDepartment of Ophthalmology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, ChinaAIM: To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages. <p>METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group(10μmol/L, 100μmol/L, 1000μmol/L)and positive control group using rats nerve growth factor(mNGF, 100ng/mL). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups. <p>RESULTS:Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance(<i>P</i><0.05).<p>CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.http://www.ies.net.cn/cn_publish/2014/8/201408003.pdfDaidzeinoptic nerve damageCaspase-3GAP-43 |
| spellingShingle | Zhong-Jun Tang Zhen-Ping Huang Wen-Jing Yang Yong-Xiang Zou Ji-Ping Cai Effect of Daidzein intravitreal injection on optic nerve injury in rats Guoji Yanke Zazhi Daidzein optic nerve damage Caspase-3 GAP-43 |
| title | Effect of Daidzein intravitreal injection on optic nerve injury in rats |
| title_full | Effect of Daidzein intravitreal injection on optic nerve injury in rats |
| title_fullStr | Effect of Daidzein intravitreal injection on optic nerve injury in rats |
| title_full_unstemmed | Effect of Daidzein intravitreal injection on optic nerve injury in rats |
| title_short | Effect of Daidzein intravitreal injection on optic nerve injury in rats |
| title_sort | effect of daidzein intravitreal injection on optic nerve injury in rats |
| topic | Daidzein optic nerve damage Caspase-3 GAP-43 |
| url | http://www.ies.net.cn/cn_publish/2014/8/201408003.pdf |
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