Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta
The infusion of the desertic plant <i>Nolana ramosissima</i> I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated...
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/3/520 |
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| author | Fredi Cifuentes Javier Palacios Jorge Bórquez Adrián Paredes Claudio Parra Alejandra Bravo Mario J. Simirgiotis |
| author_facet | Fredi Cifuentes Javier Palacios Jorge Bórquez Adrián Paredes Claudio Parra Alejandra Bravo Mario J. Simirgiotis |
| author_sort | Fredi Cifuentes |
| collection | DOAJ |
| description | The infusion of the desertic plant <i>Nolana ramosissima</i> I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The <i>N. ramosissima</i> extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound <b>5</b>,3′-dihydroxy-4′7-dimethoxyflavone presented higher relaxation effect than 100 μg/mL of <i>N. ramosissima</i> extract. The extract and the isolated metabolites from <i>N. ramosissima</i> can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium. |
| first_indexed | 2024-12-18T19:11:02Z |
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| id | doaj.art-66fb3ee4860546a6bc28597f3baacfc1 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T19:11:02Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-66fb3ee4860546a6bc28597f3baacfc12022-12-21T20:56:15ZengMDPI AGMolecules1420-30492020-01-0125352010.3390/molecules25030520molecules25030520Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat AortaFredi Cifuentes0Javier Palacios1Jorge Bórquez2Adrián Paredes3Claudio Parra4Alejandra Bravo5Mario J. Simirgiotis6Laboratorio de Fisiología Experimental (EPhyL), Instituto Antofagasta (IA), Universidad de Antofagasta, Casilla 170, Antofagasta 1271155, ChileLaboratorio de Bioquímica Aplicada, Instituto de EtnoFarmacología, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique 1110939, ChileDepartamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Casilla 170, Antofagasta 1271155, ChileDepartamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Casilla 170, Antofagasta 1271155, ChileLaboratorio de Productos Naturales y Química Medica, Facultad de Ciencias Agronómicas, Universidad de Tarapacá, Arica 1000000, ChileLaboratorio de Fisiología Experimental (EPhyL), Instituto Antofagasta (IA), Universidad de Antofagasta, Casilla 170, Antofagasta 1271155, ChileInstituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5110566, ChileThe infusion of the desertic plant <i>Nolana ramosissima</i> I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The <i>N. ramosissima</i> extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound <b>5</b>,3′-dihydroxy-4′7-dimethoxyflavone presented higher relaxation effect than 100 μg/mL of <i>N. ramosissima</i> extract. The extract and the isolated metabolites from <i>N. ramosissima</i> can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium.https://www.mdpi.com/1420-3049/25/3/520mass spectrometryhigh-performance countercurrent chromatographyflavonoidsvasodilationchilean plants |
| spellingShingle | Fredi Cifuentes Javier Palacios Jorge Bórquez Adrián Paredes Claudio Parra Alejandra Bravo Mario J. Simirgiotis Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta Molecules mass spectrometry high-performance countercurrent chromatography flavonoids vasodilation chilean plants |
| title | Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta |
| title_full | Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta |
| title_fullStr | Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta |
| title_full_unstemmed | Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta |
| title_short | Fast Isolation of Flavonoids from the Endemic Species <i>Nolana ramosissima</i> I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta |
| title_sort | fast isolation of flavonoids from the endemic species i nolana ramosissima i i m johnst and its endothelium independent relaxation effect in rat aorta |
| topic | mass spectrometry high-performance countercurrent chromatography flavonoids vasodilation chilean plants |
| url | https://www.mdpi.com/1420-3049/25/3/520 |
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