Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia
This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positiv...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2022-08-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/41521 |
| _version_ | 1811186681026445312 |
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| author | Lia Hapsari Ruth Feti Rahayuniati Siti Subandiyah |
| author_facet | Lia Hapsari Ruth Feti Rahayuniati Siti Subandiyah |
| author_sort | Lia Hapsari |
| collection | DOAJ |
| description |
This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positively infected with slight to severe intensity symptoms i.e. Pisang Berlin, Candi, Billa, Morosebo, and Mas Kripik; and five accessions were symptomless i.e. M. acuminata var. rutilifes and M. balbisiana, Pisang Ebung, Madu and Moseng. However, PCR results qualitatively confirms that all accessions were positively infected. Two sizes of amplicons were identified. Pisang Candi isolate was showing ±500 bp amplicons, whereas the others were ±1000-1100 bp. The total aligned and selected BBTV CP sequences of 36 accessions (bananas and other host species) was 450 nt. It was considered highly conserved (85.55%), low G+C content (41%), and high genetic similarities (92.47 to 100%). Phylogenetic analysis delineates isolates into two large groups, i.e. the Asian group (East Asia and South-east Asia) and the South Pacific group (South Asia, Africa and the Pacific). The findings of this study are usefull for broader applicability of further banana breeding program particularly for mitigation, selection and evaluation of banana with BBTV resistance.
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| first_indexed | 2024-04-11T13:49:17Z |
| format | Article |
| id | doaj.art-66fe6c7436f945e6bfac7004d8460eea |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-11T13:49:17Z |
| publishDate | 2022-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-66fe6c7436f945e6bfac7004d8460eea2022-12-22T04:20:43ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942022-08-0130110.4308/hjb.30.1.53-64Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from IndonesiaLia Hapsari0Ruth Feti Rahayuniati1Siti Subandiyah2Research Center for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency (BRIN), Bogor, IndonesiaFaculty of Agriculture, University of Jenderal Soedirman, Karangwangkal, Purwokerto, IndonesiaResearch Center for Biotechnology, University of Gadjah Mada, Yogyakarta, Indonesia. Faculty of Agriculture, University of Gadjah Mada, Yogyakarta, Indonesia This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positively infected with slight to severe intensity symptoms i.e. Pisang Berlin, Candi, Billa, Morosebo, and Mas Kripik; and five accessions were symptomless i.e. M. acuminata var. rutilifes and M. balbisiana, Pisang Ebung, Madu and Moseng. However, PCR results qualitatively confirms that all accessions were positively infected. Two sizes of amplicons were identified. Pisang Candi isolate was showing ±500 bp amplicons, whereas the others were ±1000-1100 bp. The total aligned and selected BBTV CP sequences of 36 accessions (bananas and other host species) was 450 nt. It was considered highly conserved (85.55%), low G+C content (41%), and high genetic similarities (92.47 to 100%). Phylogenetic analysis delineates isolates into two large groups, i.e. the Asian group (East Asia and South-east Asia) and the South Pacific group (South Asia, Africa and the Pacific). The findings of this study are usefull for broader applicability of further banana breeding program particularly for mitigation, selection and evaluation of banana with BBTV resistance. https://journal.ipb.ac.id/index.php/hayati/article/view/41521 |
| spellingShingle | Lia Hapsari Ruth Feti Rahayuniati Siti Subandiyah Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia Hayati Journal of Biosciences |
| title | Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia |
| title_full | Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia |
| title_fullStr | Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia |
| title_full_unstemmed | Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia |
| title_short | Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia |
| title_sort | banana bunchy top virus molecular confirmation and dna s phylogenetic of some banana isolates from indonesia |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/41521 |
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