| Summary: | Metabolomics strategies are important tools to get holistic chemical information from a system, but they are scarcely applied to endophytic fungi to understand their chemical profiles of biosynthesized metabolites. Here <i>Penicillium</i> sp. was cultured using One Strain Many Compounds (OSMAC) conditions as a model system to demonstrate how this strategy can help in understanding metabolic profiles and determining bioactive metabolites with the application of metabolomics and statistical analyses, as well as molecular networking. <i>Penicillium</i> sp. was fermented in different culture media and the crude extracts from mycelial biomass (CEm) and broth (CEb) were obtained, evaluated against bacterial strains (<i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>), and the metabolomic profiles by LC-DAD-MS were obtained and chemometrics statistical analyses were applied. The CEm and CEb extracts presented different chemical profiles and antibacterial activities; the highest activities observed were against <i>S. aureus</i> from CEm (MIC = 16, 64, and 128 µg/mL). The antibacterial properties from the extracts were impacted for culture media from which the strain was fermented. From the Volcano plot analysis, it was possible to determine statistically the most relevant features for the antibacterial activity, which were also confirmed from biplots of PCA as strong features for the bioactive extracts. These compounds included <b>75</b> (13-oxoverruculogen isomer), <b>78</b> (austalide P acid), <b>87</b> (austalide L or W), <b>88</b> (helvamide), <b>92</b> (viridicatumtoxin A), <b>96</b> (austalide P), <b>101</b> (dihydroaustalide K), <b>106</b> (austalide k), <b>110</b> (spirohexaline), and <b>112</b> (pre-viridicatumtoxin). Thus, these features included diketopiperazines, meroterpenoids, and polyketides, such as indole alkaloids, austalides, and viridicatumtoxin A, a rare tetracycline.
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