Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus
Rice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02322/full |
| _version_ | 1819163852591333376 |
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| author | Zhiyang Liu Zhiyang Liu Xuejuan Li Xuejuan Li Feng Sun Feng Sun Tong Zhou Tong Zhou Yijun Zhou Yijun Zhou |
| author_facet | Zhiyang Liu Zhiyang Liu Xuejuan Li Xuejuan Li Feng Sun Feng Sun Tong Zhou Tong Zhou Yijun Zhou Yijun Zhou |
| author_sort | Zhiyang Liu |
| collection | DOAJ |
| description | Rice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in rice. Our investigation showed that overexpression of OsCIPK30 in rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease. |
| first_indexed | 2024-12-22T17:50:43Z |
| format | Article |
| id | doaj.art-6700ddbccd5747fe9e71d0dd91cf3dbd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T17:50:43Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6700ddbccd5747fe9e71d0dd91cf3dbd2022-12-21T18:18:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-11-01810.3389/fmicb.2017.02322304228Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virusZhiyang Liu0Zhiyang Liu1Xuejuan Li2Xuejuan Li3Feng Sun4Feng Sun5Tong Zhou6Tong Zhou7Yijun Zhou8Yijun Zhou9Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, ChinaScientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, ChinaScientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, ChinaScientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, ChinaScientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, ChinaScientific Observation and Experimental Station of Crop Pests in Nanjing, Ministry of Agriculture, Nanjing, ChinaRice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in rice. Our investigation showed that overexpression of OsCIPK30 in rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02322/fullRice stripe virusNS3interactionOsCIPK30pathogenesis-related genes |
| spellingShingle | Zhiyang Liu Zhiyang Liu Xuejuan Li Xuejuan Li Feng Sun Feng Sun Tong Zhou Tong Zhou Yijun Zhou Yijun Zhou Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus Frontiers in Microbiology Rice stripe virus NS3 interaction OsCIPK30 pathogenesis-related genes |
| title | Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus |
| title_full | Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus |
| title_fullStr | Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus |
| title_full_unstemmed | Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus |
| title_short | Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus |
| title_sort | overexpression of oscipk30 enhances plant tolerance to rice stripe virus |
| topic | Rice stripe virus NS3 interaction OsCIPK30 pathogenesis-related genes |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02322/full |
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