A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice
Bacterial blight (BB) induced by Xanthomonas oryzae pv. oryzae (Xoo) is a devastating bacterial disease in rice. The use of disease resistance (R) genes is the most efficient method to control BB. Members of the nucleotide-binding domain and leucine-rich repeat containing protein (NLR) family have s...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1037901/full |
| _version_ | 1811179978932355072 |
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| author | Yuanda Lu Yuanda Lu Qiaofang Zhong Suqin Xiao Bo Wang Xue Ke Yun Zhang Fuyou Yin Dunyu Zhang Cong Jiang Li Liu Jinlu Li Tengqiong Yu Lingxian Wang Zaiquan Cheng Ling Chen |
| author_facet | Yuanda Lu Yuanda Lu Qiaofang Zhong Suqin Xiao Bo Wang Xue Ke Yun Zhang Fuyou Yin Dunyu Zhang Cong Jiang Li Liu Jinlu Li Tengqiong Yu Lingxian Wang Zaiquan Cheng Ling Chen |
| author_sort | Yuanda Lu |
| collection | DOAJ |
| description | Bacterial blight (BB) induced by Xanthomonas oryzae pv. oryzae (Xoo) is a devastating bacterial disease in rice. The use of disease resistance (R) genes is the most efficient method to control BB. Members of the nucleotide-binding domain and leucine-rich repeat containing protein (NLR) family have significant roles in plant defense. In this study, Xa47, a new bacterial blight R gene encoding a typical NLR, was isolated from G252 rice material, and XA47 was localized in the nucleus and cytoplasm. Among 180 rice materials tested, Xa47 was discovered in certain BB-resistant materials. Compared with the wild-type G252, the knockout mutants of Xa47 was more susceptible to Xoo. By contrast, overexpression of Xa47 in the susceptible rice material JG30 increased BB resistance. The findings indicate that Xa47 positively regulates the Xoo stress response. Consequently, Xa47 may have application potential in the genetic improvement of plant disease resistance. The molecular mechanism of Xa47 regulation merits additional examination. |
| first_indexed | 2024-04-11T06:42:29Z |
| format | Article |
| id | doaj.art-6703585eefdf43569f3a47d2c415d17f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T06:42:29Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6703585eefdf43569f3a47d2c415d17f2022-12-22T04:39:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10379011037901A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in riceYuanda Lu0Yuanda Lu1Qiaofang Zhong2Suqin Xiao3Bo Wang4Xue Ke5Yun Zhang6Fuyou Yin7Dunyu Zhang8Cong Jiang9Li Liu10Jinlu Li11Tengqiong Yu12Lingxian Wang13Zaiquan Cheng14Ling Chen15Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, ChinaBacterial blight (BB) induced by Xanthomonas oryzae pv. oryzae (Xoo) is a devastating bacterial disease in rice. The use of disease resistance (R) genes is the most efficient method to control BB. Members of the nucleotide-binding domain and leucine-rich repeat containing protein (NLR) family have significant roles in plant defense. In this study, Xa47, a new bacterial blight R gene encoding a typical NLR, was isolated from G252 rice material, and XA47 was localized in the nucleus and cytoplasm. Among 180 rice materials tested, Xa47 was discovered in certain BB-resistant materials. Compared with the wild-type G252, the knockout mutants of Xa47 was more susceptible to Xoo. By contrast, overexpression of Xa47 in the susceptible rice material JG30 increased BB resistance. The findings indicate that Xa47 positively regulates the Xoo stress response. Consequently, Xa47 may have application potential in the genetic improvement of plant disease resistance. The molecular mechanism of Xa47 regulation merits additional examination.https://www.frontiersin.org/articles/10.3389/fpls.2022.1037901/fullXa47NLRXanthomonas oryzae pv. oryzaebroad-spectrum resistancebacterial blight |
| spellingShingle | Yuanda Lu Yuanda Lu Qiaofang Zhong Suqin Xiao Bo Wang Xue Ke Yun Zhang Fuyou Yin Dunyu Zhang Cong Jiang Li Liu Jinlu Li Tengqiong Yu Lingxian Wang Zaiquan Cheng Ling Chen A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice Frontiers in Plant Science Xa47 NLR Xanthomonas oryzae pv. oryzae broad-spectrum resistance bacterial blight |
| title | A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice |
| title_full | A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice |
| title_fullStr | A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice |
| title_full_unstemmed | A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice |
| title_short | A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice |
| title_sort | new nlr disease resistance gene xa47 confers durable and broad spectrum resistance to bacterial blight in rice |
| topic | Xa47 NLR Xanthomonas oryzae pv. oryzae broad-spectrum resistance bacterial blight |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1037901/full |
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