Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds
Objective: To investigate the antioxidant, anti-α-glucosidase and pancreatic β-cell protective potential of Ensete superbum (E. superbum) seeds. Methods: A variety of in vitro assays including radical scavenging, reducing power potential, phenolic content determination, α-glucosidase assay and pancr...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-02-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116310905 |
| _version_ | 1819080309319139328 |
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| author | Solomon Habtemariam George Kabakasseril Varghese |
| author_facet | Solomon Habtemariam George Kabakasseril Varghese |
| author_sort | Solomon Habtemariam |
| collection | DOAJ |
| description | Objective: To investigate the antioxidant, anti-α-glucosidase and pancreatic β-cell protective potential of Ensete superbum (E. superbum) seeds.
Methods: A variety of in vitro assays including radical scavenging, reducing power potential, phenolic content determination, α-glucosidase assay and pancreatic β-cell (1.4E7 cells) viability were employed for assessing the effect of methanolic extract of E. superbum seeds.
Results: The radical scavenging and reducing power effects comparable with the standard rutin were obtained while the enzyme inhibitory activity of the extract was 68-fold better than the standard antidiabetic drug, acarbose. The seed extract of E. superbum was packed-full of polyphenols with mean percentage gallic acid equivalent value of (38.2 ± 1.8) (n = 3). The protection of pancreatic cells from massive onslaught of hydrogen peroxide was far superior to that obtained for rutin.
Conclusions: The reputed antidiabetic therapeutic uses of the seeds extract of E. superbum may be justified on the basis of inhibition of carbohydrate enzymes, antioxidant effects and pancreatic β-cell protection. |
| first_indexed | 2024-12-21T19:42:50Z |
| format | Article |
| id | doaj.art-67038759443441a7b72d8735e6122642 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-21T19:42:50Z |
| publishDate | 2017-02-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-67038759443441a7b72d8735e61226422022-12-21T18:52:24ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-02-017212112510.1016/j.apjtb.2016.10.012Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seedsSolomon Habtemariam0George Kabakasseril Varghese1Pharmacognosy Research Laboratories & Herbal Analysis Services, Medway School of Science, University of Greenwich, Chatham-Maritime, Kent ME4 4TB, UKDepartment of Botany, St. Berchmans' College, Changanassery, Kottayam 686101, Kerala, IndiaObjective: To investigate the antioxidant, anti-α-glucosidase and pancreatic β-cell protective potential of Ensete superbum (E. superbum) seeds. Methods: A variety of in vitro assays including radical scavenging, reducing power potential, phenolic content determination, α-glucosidase assay and pancreatic β-cell (1.4E7 cells) viability were employed for assessing the effect of methanolic extract of E. superbum seeds. Results: The radical scavenging and reducing power effects comparable with the standard rutin were obtained while the enzyme inhibitory activity of the extract was 68-fold better than the standard antidiabetic drug, acarbose. The seed extract of E. superbum was packed-full of polyphenols with mean percentage gallic acid equivalent value of (38.2 ± 1.8) (n = 3). The protection of pancreatic cells from massive onslaught of hydrogen peroxide was far superior to that obtained for rutin. Conclusions: The reputed antidiabetic therapeutic uses of the seeds extract of E. superbum may be justified on the basis of inhibition of carbohydrate enzymes, antioxidant effects and pancreatic β-cell protection.http://www.sciencedirect.com/science/article/pii/S2221169116310905Ensete superbumMusaceaeSeedsAntioxidantPancreatic beta-cell protectionAlpha-glucosidase inhibition |
| spellingShingle | Solomon Habtemariam George Kabakasseril Varghese Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds Asian Pacific Journal of Tropical Biomedicine Ensete superbum Musaceae Seeds Antioxidant Pancreatic beta-cell protection Alpha-glucosidase inhibition |
| title | Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds |
| title_full | Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds |
| title_fullStr | Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds |
| title_full_unstemmed | Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds |
| title_short | Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds |
| title_sort | antioxidant anti alpha glucosidase and pancreatic beta cell protective effects of methanolic extract of ensete superbum cheesm seeds |
| topic | Ensete superbum Musaceae Seeds Antioxidant Pancreatic beta-cell protection Alpha-glucosidase inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116310905 |
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