Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication
Summary: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019. Few studies have compared replication dynamics and host responses to SARS-CoV-2 in cell lines from different tissues and species. Therefore, we investigated the role of tissue ty...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221015236 |
| _version_ | 1798024742530187264 |
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| author | Elizabeth Geerling Amanda N. Pinski Taylor E. Stone Richard J. DiPaolo Michael Z. Zulu Kevin J. Maroney James D. Brien Ilhem Messaoudi Amelia K. Pinto |
| author_facet | Elizabeth Geerling Amanda N. Pinski Taylor E. Stone Richard J. DiPaolo Michael Z. Zulu Kevin J. Maroney James D. Brien Ilhem Messaoudi Amelia K. Pinto |
| author_sort | Elizabeth Geerling |
| collection | DOAJ |
| description | Summary: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019. Few studies have compared replication dynamics and host responses to SARS-CoV-2 in cell lines from different tissues and species. Therefore, we investigated the role of tissue type and antiviral genes during SARS-CoV-2 infection in nonhuman primate (kidney) and human (liver, respiratory epithelial, gastric) cell lines. We report different viral growth kinetics and release among the cell lines despite comparable ACE2 expression. Transcriptomics revealed that absence of STAT1 in nonhuman primate cells appeared to enhance inflammatory responses without effecting infectious viral titer. Deletion of RL-6 in respiratory epithelial cells increased viral replication. Impaired infectious virus release was detected in Huh7 but not Huh7.5 cells, suggesting a role for RIG1. Gastric cells MKN45 exhibited robust antiviral gene expression and supported viral replication. Data here provide insight into molecular pathogenesis of and alternative cell lines for studying SARS-CoV-2 infection. |
| first_indexed | 2024-04-11T18:07:33Z |
| format | Article |
| id | doaj.art-6706e14fa6744fd3962e3f683aaae492 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T18:07:33Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6706e14fa6744fd3962e3f683aaae4922022-12-22T04:10:17ZengElsevieriScience2589-00422022-01-01251103553Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replicationElizabeth Geerling0Amanda N. Pinski1Taylor E. Stone2Richard J. DiPaolo3Michael Z. Zulu4Kevin J. Maroney5James D. Brien6Ilhem Messaoudi7Amelia K. Pinto8Department of Molecular Microbiology and Immunology, Saint Louis University, St Louis, MO 63103, USADepartment of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA 92697, USADepartment of Molecular Microbiology and Immunology, Saint Louis University, St Louis, MO 63103, USADepartment of Molecular Microbiology and Immunology, Saint Louis University, St Louis, MO 63103, USADepartment of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA 92697, USADepartment of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA 92697, USADepartment of Molecular Microbiology and Immunology, Saint Louis University, St Louis, MO 63103, USADepartment of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA 92697, USA; Corresponding authorDepartment of Molecular Microbiology and Immunology, Saint Louis University, St Louis, MO 63103, USA; Corresponding authorSummary: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019. Few studies have compared replication dynamics and host responses to SARS-CoV-2 in cell lines from different tissues and species. Therefore, we investigated the role of tissue type and antiviral genes during SARS-CoV-2 infection in nonhuman primate (kidney) and human (liver, respiratory epithelial, gastric) cell lines. We report different viral growth kinetics and release among the cell lines despite comparable ACE2 expression. Transcriptomics revealed that absence of STAT1 in nonhuman primate cells appeared to enhance inflammatory responses without effecting infectious viral titer. Deletion of RL-6 in respiratory epithelial cells increased viral replication. Impaired infectious virus release was detected in Huh7 but not Huh7.5 cells, suggesting a role for RIG1. Gastric cells MKN45 exhibited robust antiviral gene expression and supported viral replication. Data here provide insight into molecular pathogenesis of and alternative cell lines for studying SARS-CoV-2 infection.http://www.sciencedirect.com/science/article/pii/S2589004221015236ImmunologyMicrobiologyVirology |
| spellingShingle | Elizabeth Geerling Amanda N. Pinski Taylor E. Stone Richard J. DiPaolo Michael Z. Zulu Kevin J. Maroney James D. Brien Ilhem Messaoudi Amelia K. Pinto Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication iScience Immunology Microbiology Virology |
| title | Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication |
| title_full | Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication |
| title_fullStr | Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication |
| title_full_unstemmed | Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication |
| title_short | Roles of antiviral sensing and type I interferon signaling in the restriction of SARS-CoV-2 replication |
| title_sort | roles of antiviral sensing and type i interferon signaling in the restriction of sars cov 2 replication |
| topic | Immunology Microbiology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221015236 |
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