Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans
N-carboxymethyl-lysine (CML) and other dietary advanced glycation end-products (AGEs) are chemically modified amino acids with potential toxicological effects putatively related to their affinity with the receptor for AGEs (RAGE). The goal of this study was to determine the postprandial kinetics of...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/9/1890 |
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| author | Cynthia Helou Matheus Thomaz Nogueira Silva Lima Céline Niquet-Leridon Philippe Jacolot Eric Boulanger Florian Delguste Axel Guilbaud Michael Genin Pauline M. Anton Carine Delayre-Orthez Tatiana Papazian Michael Howsam Frédéric J. Tessier |
| author_facet | Cynthia Helou Matheus Thomaz Nogueira Silva Lima Céline Niquet-Leridon Philippe Jacolot Eric Boulanger Florian Delguste Axel Guilbaud Michael Genin Pauline M. Anton Carine Delayre-Orthez Tatiana Papazian Michael Howsam Frédéric J. Tessier |
| author_sort | Cynthia Helou |
| collection | DOAJ |
| description | N-carboxymethyl-lysine (CML) and other dietary advanced glycation end-products (AGEs) are chemically modified amino acids with potential toxicological effects putatively related to their affinity with the receptor for AGEs (RAGE). The goal of this study was to determine the postprandial kinetics of CML in both rodents and humans and, in the latter, to evaluate their relationship with the soluble RAGE isoforms (sRAGE). Four gavage solutions containing different forms of CML were given to rats, and blood was collected over 8 h. Three different breakfasts containing dietary CML (dCML) were administered to 20 healthy volunteers, and blood was collected over 2 h. Concentrations of CML, CEL, and lysine were quantified in plasma and human meals by LC-MS/MS, and sRAGE was determined in human plasma by ELISA. The results showed that dCML did not affect the concentrations of circulating protein-bound CML and that only free CML increased in plasma, with a postprandial peak at 90 to 120 min. In humans, the postprandial plasmatic sRAGE concentration decreased independently of the dAGE content of the breakfasts. This study confirms reports of the inverse postprandial relationship between plasmatic free CML and sRAGE, though this requires further investigation for causality to be established. |
| first_indexed | 2024-03-10T03:49:44Z |
| format | Article |
| id | doaj.art-67098f06a57b4afeaec740d7adb58839 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:49:44Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-67098f06a57b4afeaec740d7adb588392023-11-23T08:59:52ZengMDPI AGNutrients2072-66432022-04-01149189010.3390/nu14091890Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in HumansCynthia Helou0Matheus Thomaz Nogueira Silva Lima1Céline Niquet-Leridon2Philippe Jacolot3Eric Boulanger4Florian Delguste5Axel Guilbaud6Michael Genin7Pauline M. Anton8Carine Delayre-Orthez9Tatiana Papazian10Michael Howsam11Frédéric J. Tessier12Department of Nutrition, Faculty of Pharmacy, Saint Joseph University of Beirut, Beirut 1004 2020, LebanonU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceULR 7519, Equipe PETALES, Institut Polytechnique UniLaSalle, Université d’Artois, F-60026 Beauvais, FranceULR 7519, Equipe PETALES, Institut Polytechnique UniLaSalle, Université d’Artois, F-60026 Beauvais, FranceU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceULR 2694-METRICS: Évaluation des Technologies de Santé et des Pratiques Médicales, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceULR 7519, Equipe PETALES, Institut Polytechnique UniLaSalle, Université d’Artois, F-60026 Beauvais, FranceULR 7519, Equipe PETALES, Institut Polytechnique UniLaSalle, Université d’Artois, F-60026 Beauvais, FranceDepartment of Nutrition, Faculty of Pharmacy, Saint Joseph University of Beirut, Beirut 1004 2020, LebanonU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceU1167—RID—AGE—Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, FranceN-carboxymethyl-lysine (CML) and other dietary advanced glycation end-products (AGEs) are chemically modified amino acids with potential toxicological effects putatively related to their affinity with the receptor for AGEs (RAGE). The goal of this study was to determine the postprandial kinetics of CML in both rodents and humans and, in the latter, to evaluate their relationship with the soluble RAGE isoforms (sRAGE). Four gavage solutions containing different forms of CML were given to rats, and blood was collected over 8 h. Three different breakfasts containing dietary CML (dCML) were administered to 20 healthy volunteers, and blood was collected over 2 h. Concentrations of CML, CEL, and lysine were quantified in plasma and human meals by LC-MS/MS, and sRAGE was determined in human plasma by ELISA. The results showed that dCML did not affect the concentrations of circulating protein-bound CML and that only free CML increased in plasma, with a postprandial peak at 90 to 120 min. In humans, the postprandial plasmatic sRAGE concentration decreased independently of the dAGE content of the breakfasts. This study confirms reports of the inverse postprandial relationship between plasmatic free CML and sRAGE, though this requires further investigation for causality to be established.https://www.mdpi.com/2072-6643/14/9/1890glycationMaillard reactioncarboxymethyllysinelysinesRAGE |
| spellingShingle | Cynthia Helou Matheus Thomaz Nogueira Silva Lima Céline Niquet-Leridon Philippe Jacolot Eric Boulanger Florian Delguste Axel Guilbaud Michael Genin Pauline M. Anton Carine Delayre-Orthez Tatiana Papazian Michael Howsam Frédéric J. Tessier Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans Nutrients glycation Maillard reaction carboxymethyllysine lysine sRAGE |
| title | Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans |
| title_full | Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans |
| title_fullStr | Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans |
| title_full_unstemmed | Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans |
| title_short | Plasma Levels of Free <i>N</i><i><sup>Ɛ</sup></i>-Carboxymethyllysine (CML) after Different Oral Doses of CML in Rats and after the Intake of Different Breakfasts in Humans: Postprandial Plasma Level of sRAGE in Humans |
| title_sort | plasma levels of free i n i i sup e sup i carboxymethyllysine cml after different oral doses of cml in rats and after the intake of different breakfasts in humans postprandial plasma level of srage in humans |
| topic | glycation Maillard reaction carboxymethyllysine lysine sRAGE |
| url | https://www.mdpi.com/2072-6643/14/9/1890 |
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