Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells
Human induced Pluripotent Stem Cells (hiPSCs) represent an invaluable source of primary cells to investigate development, establish cell and disease models, provide material for regenerative medicine and allow more physiological high-content screenings. Here, we generated three healthy hiPSC control...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124000485 |
| _version_ | 1797299686939295744 |
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| author | Mikaël Boullé Alix Boucharlat Ambre Leleu Céline Banal Aurélie Coussement Marcel Hollenstein Frank Yates Nathalie Lefort Fabrice Agou |
| author_facet | Mikaël Boullé Alix Boucharlat Ambre Leleu Céline Banal Aurélie Coussement Marcel Hollenstein Frank Yates Nathalie Lefort Fabrice Agou |
| author_sort | Mikaël Boullé |
| collection | DOAJ |
| description | Human induced Pluripotent Stem Cells (hiPSCs) represent an invaluable source of primary cells to investigate development, establish cell and disease models, provide material for regenerative medicine and allow more physiological high-content screenings. Here, we generated three healthy hiPSC control lines - IPi001-A/B/C - from primary amniotic fluid cells (AFCs), an infrequently used source of cells, which can be readily obtained from amniocentesis for the prenatal diagnosis of numerous genetic disorders. These AFCs were reprogrammed by non-integrative viral transduction. The resulting hiPSCs displayed normal karyotype and expressed classic pluripotency hallmarks. |
| first_indexed | 2024-03-07T22:55:19Z |
| format | Article |
| id | doaj.art-670abe90fddf4373896cf392088b9aec |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-07T22:55:19Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-670abe90fddf4373896cf392088b9aec2024-02-23T04:59:19ZengElsevierStem Cell Research1873-50612024-04-0176103350Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cellsMikaël Boullé0Alix Boucharlat1Ambre Leleu2Céline Banal3Aurélie Coussement4Marcel Hollenstein5Frank Yates6Nathalie Lefort7Fabrice Agou8Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France; Corresponding authors.Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, FranceSup'Biotech-CEA/DRF/IBFJ/SEPIA, 92260 Fontenay-aux-Roses, FranceUniversité Paris-Cité, iPSC Core Facility, Institut Imagine, INSERM UMR U1163, 75015 Paris, FranceService de Cytogénétique, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Université Paris-Cité, 75014 Paris, FranceLaboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, FranceSup'Biotech-CEA/DRF/IBFJ/SEPIA, 92260 Fontenay-aux-Roses, FranceUniversité Paris-Cité, iPSC Core Facility, Institut Imagine, INSERM UMR U1163, 75015 Paris, FranceChemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France; Corresponding authors.Human induced Pluripotent Stem Cells (hiPSCs) represent an invaluable source of primary cells to investigate development, establish cell and disease models, provide material for regenerative medicine and allow more physiological high-content screenings. Here, we generated three healthy hiPSC control lines - IPi001-A/B/C - from primary amniotic fluid cells (AFCs), an infrequently used source of cells, which can be readily obtained from amniocentesis for the prenatal diagnosis of numerous genetic disorders. These AFCs were reprogrammed by non-integrative viral transduction. The resulting hiPSCs displayed normal karyotype and expressed classic pluripotency hallmarks.http://www.sciencedirect.com/science/article/pii/S1873506124000485 |
| spellingShingle | Mikaël Boullé Alix Boucharlat Ambre Leleu Céline Banal Aurélie Coussement Marcel Hollenstein Frank Yates Nathalie Lefort Fabrice Agou Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells Stem Cell Research |
| title | Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| title_full | Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| title_fullStr | Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| title_full_unstemmed | Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| title_short | Generation of IPi001-A/B/C human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| title_sort | generation of ipi001 a b c human induced pluripotent stem cell lines from healthy amniotic fluid cells |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124000485 |
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