A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas
The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illum...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1385369 |
| _version_ | 1818908933273681920 |
|---|---|
| author | Manosha Perera Nezar Noor Al-hebshi Irosha Perera Deepak Ipe Glen C. Ulett David J. Speicher Tsute Chen Newell W. Johnson |
| author_facet | Manosha Perera Nezar Noor Al-hebshi Irosha Perera Deepak Ipe Glen C. Ulett David J. Speicher Tsute Chen Newell W. Johnson |
| author_sort | Manosha Perera |
| collection | DOAJ |
| description | The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE’s named species sequences as reference. Downstream analyses were performed using QIIME™ and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with Candida and Malassezia making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera Candida, Hannaella, and Gibberella were overrepresented in OSCC; Alternaria and Trametes were more abundant in FEP. Species-wise, Candida albicans, Candida etchellsii, and a Hannaella luteola–like species were enriched in OSCC, while a Hanseniaspora uvarum–like species, Malassezia restricta, and Aspergillus tamarii were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by C. albicans was found in association with OSCC, a finding worth further investigation. |
| first_indexed | 2024-12-19T22:18:53Z |
| format | Article |
| id | doaj.art-670b40e5f7884393b0fdfd6b8cfac303 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-19T22:18:53Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-670b40e5f7884393b0fdfd6b8cfac3032022-12-21T20:03:41ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-01-019110.1080/20002297.2017.13853691385369A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomasManosha Perera0Nezar Noor Al-hebshi1Irosha Perera2Deepak Ipe3Glen C. Ulett4David J. Speicher5Tsute Chen6Newell W. Johnson7Griffith UniversityTemple UniversityThe National Dental Hospital (Teaching)Griffith UniversityGriffith UniversityGriffith UniversityForsyth InstituteGriffith UniversityThe aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE’s named species sequences as reference. Downstream analyses were performed using QIIME™ and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with Candida and Malassezia making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera Candida, Hannaella, and Gibberella were overrepresented in OSCC; Alternaria and Trametes were more abundant in FEP. Species-wise, Candida albicans, Candida etchellsii, and a Hannaella luteola–like species were enriched in OSCC, while a Hanseniaspora uvarum–like species, Malassezia restricta, and Aspergillus tamarii were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by C. albicans was found in association with OSCC, a finding worth further investigation.http://dx.doi.org/10.1080/20002297.2017.1385369Fungimycobiomecarcinomahigh-throughput nucleotide sequencingDNA ribosomal spacermicrobiomemouthsquamous cell |
| spellingShingle | Manosha Perera Nezar Noor Al-hebshi Irosha Perera Deepak Ipe Glen C. Ulett David J. Speicher Tsute Chen Newell W. Johnson A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas Journal of Oral Microbiology Fungi mycobiome carcinoma high-throughput nucleotide sequencing DNA ribosomal spacer microbiome mouth squamous cell |
| title | A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas |
| title_full | A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas |
| title_fullStr | A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas |
| title_full_unstemmed | A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas |
| title_short | A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas |
| title_sort | dysbiotic mycobiome dominated by candida albicans is identified within oral squamous cell carcinomas |
| topic | Fungi mycobiome carcinoma high-throughput nucleotide sequencing DNA ribosomal spacer microbiome mouth squamous cell |
| url | http://dx.doi.org/10.1080/20002297.2017.1385369 |
| work_keys_str_mv | AT manoshaperera adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT nezarnooralhebshi adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT iroshaperera adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT deepakipe adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT glenculett adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT davidjspeicher adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT tsutechen adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT newellwjohnson adysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT manoshaperera dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT nezarnooralhebshi dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT iroshaperera dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT deepakipe dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT glenculett dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT davidjspeicher dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT tsutechen dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas AT newellwjohnson dysbioticmycobiomedominatedbycandidaalbicansisidentifiedwithinoralsquamouscellcarcinomas |