INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS
Abstract. Involvement of dendritic cells (DC) into switching of immune response to T-independent type 2 antigens (TI2 antigens) is poorly studied. The present study addressed some interactions between DCs and TI2-type antigens, i.e., with Streptococcus pneumoniae polysaccharide type 3 (S3), and poly...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/295 |
| _version_ | 1826534324089913344 |
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| author | D. A. Khochenkov M. V. Gavrilova |
| author_facet | D. A. Khochenkov M. V. Gavrilova |
| author_sort | D. A. Khochenkov |
| collection | DOAJ |
| description | Abstract. Involvement of dendritic cells (DC) into switching of immune response to T-independent type 2 antigens (TI2 antigens) is poorly studied. The present study addressed some interactions between DCs and TI2-type antigens, i.e., with Streptococcus pneumoniae polysaccharide type 3 (S3), and polyvinylpyrrolidone (PVP, mw. of 360 kDa), as well as a role of antigen-loaded DCs for triggering the immune response. Shortterm treatment of DCs with TI-2 antigens induced their activation manifesting as an increase in numbers of CD80- and CD86-positive cells under the cell culture conditions. DCs loaded with TI-2 antigens were then mixed with normal murine splenocytes and cultured in complete RPMI 1640 medium for 4 days. The numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) were determined by ELISPOT assay. Supplementation with TI2-treated DCs induced a 2.0 to 2.7-fold increase in specific immune response. Antigen-induced polyclonal response was enhanced by 40%. These data suggest a direct interaction between DCs and TI-2 antigens, and their presentation to murine splenocytes, thus leading to both specific and polyclonal B cell activation. B-1 cells are shown to play a main role in such response. Separation of loaded DC and splenocytes by semi-permeable membranes caused inhibition of this immune response. |
| first_indexed | 2024-03-08T05:51:36Z |
| format | Article |
| id | doaj.art-670b55b7c96041419cc505d2bf6fd334 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:21:12Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-670b55b7c96041419cc505d2bf6fd3342025-03-11T17:59:02ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01141-2515810.15789/1563-0625-2012-1-2-51-58292INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENSD. A. Khochenkov0M. V. Gavrilova1НИИ вакцин и сывороток имени И.И. Мечникова РАМН, МоскваНИИ вакцин и сывороток имени И.И. Мечникова РАМН, МоскваAbstract. Involvement of dendritic cells (DC) into switching of immune response to T-independent type 2 antigens (TI2 antigens) is poorly studied. The present study addressed some interactions between DCs and TI2-type antigens, i.e., with Streptococcus pneumoniae polysaccharide type 3 (S3), and polyvinylpyrrolidone (PVP, mw. of 360 kDa), as well as a role of antigen-loaded DCs for triggering the immune response. Shortterm treatment of DCs with TI-2 antigens induced their activation manifesting as an increase in numbers of CD80- and CD86-positive cells under the cell culture conditions. DCs loaded with TI-2 antigens were then mixed with normal murine splenocytes and cultured in complete RPMI 1640 medium for 4 days. The numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) were determined by ELISPOT assay. Supplementation with TI2-treated DCs induced a 2.0 to 2.7-fold increase in specific immune response. Antigen-induced polyclonal response was enhanced by 40%. These data suggest a direct interaction between DCs and TI-2 antigens, and their presentation to murine splenocytes, thus leading to both specific and polyclonal B cell activation. B-1 cells are shown to play a main role in such response. Separation of loaded DC and splenocytes by semi-permeable membranes caused inhibition of this immune response.https://www.mimmun.ru/mimmun/article/view/295дендритные клеткиполисахарид s3 streptococcus pneumoniaeполивинипирролидонт-независимые антигены типа 2индукция иммунного ответа in vitro |
| spellingShingle | D. A. Khochenkov M. V. Gavrilova INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS Медицинская иммунология дендритные клетки полисахарид s3 streptococcus pneumoniae поливинипирролидон т-независимые антигены типа 2 индукция иммунного ответа in vitro |
| title | INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS |
| title_full | INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS |
| title_fullStr | INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS |
| title_full_unstemmed | INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS |
| title_short | INTERACTION BETWEEN DENDRITIC CELLS AND B CELLS DURING IMMUNE RESPONSE TO T CELL-INDEPENDENT ANTIGENS |
| title_sort | interaction between dendritic cells and b cells during immune response to t cell independent antigens |
| topic | дендритные клетки полисахарид s3 streptococcus pneumoniae поливинипирролидон т-независимые антигены типа 2 индукция иммунного ответа in vitro |
| url | https://www.mimmun.ru/mimmun/article/view/295 |
| work_keys_str_mv | AT dakhochenkov interactionbetweendendriticcellsandbcellsduringimmuneresponsetotcellindependentantigens AT mvgavrilova interactionbetweendendriticcellsandbcellsduringimmuneresponsetotcellindependentantigens |